2006 Fiscal Year Final Research Report Summary
Anti-angiogenetic therapy for malignant gliomas by the magnetofection of siRNA.
| Project/Area Number |
17591508
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Cerebral neurosurgery
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| Research Institution | University of Fukui |
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Principal Investigator |
KUBOTA Toshihiko University of Fukui, Faculty of Medical Sciences, Professor, 医学部, 教授 (70092781)
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| Co-Investigator(Kenkyū-buntansha) |
NAKAGAWA Takao University of Fukui Hospital, Lecturer, 医学部附属病院, 講師 (40217675)
ARISHIMA Hidetaka University of Fukui Hospital, Assistant Professor, 医学部附属病院, 助手 (70293420)
IDO Kazunori University of Fukui Hospital, Assistant Professor, 医学部附属病院, 助手 (00322123)
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| Project Period (FY) |
2005 – 2006
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| Keywords | glioma / VEGF / RNA interference / Magnetofection / HuR / angiogenesis |
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| Research Abstract |
1) Transfection of siRNA for VEGF-A to U87 and U251 glioma cells decreased their VEGF-A expression.
2) Tube formation of HUVECs on fibrin gel was observed in culture media(CM) from U87 and U251 glioma cells, but not in CM from glioma cells transfected by siRNA for VEGF-A.
3) siRNA for VEGF-A by magentofection could be transfected into cultured glioma cells faster than by conventional methods.
4) Expression of VEGF-A was higher in malignant gliomas compared to low-grade gliomas.
5) Expression of HuR was higher in malignant gliomas compared to low-grade gliomas.
6) Transfection of siRNA for HuR to U87 and U251 glioma cells decreased their proliferation as well as VEGF-A expression.
7) Leptomycin B, which is one of HuR inhibitor, decreased the proliferation and VEGF-A expression in U87 and U251 glioma cells.
8) Magnetofection of siRNA for VEGF-A impregnated in atherocollagen did not inhibit the growth of the implanted U87 glioma under the skin of nude mice.
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Research Products
(22 results)
