2007 Fiscal Year Final Research Report Summary

Intrinsic stem cell promote neuronal regeneration by oligomerization of A β 1-42monomer

Research Project

Project/Area Number	17591513
Research Category	Grant-in-Aid for Scientific Research (C)
Allocation Type	Single-year Grants
Section	一般
Research Field	Cerebral neurosurgery
Research Institution	Kobe University
Principal Investigator	KONDOH Takeshi Kobe University, School of Medicine, Associate Professor (50273769)
Co-Investigator(Kenkyū-buntansha)	MATSUMOTOQ Akira Foundation for Biomedical Research and Innovation, 脳疾患病態解析部, Director (80181759) KOHMURA Eiji Kobe University, Graduate School of Medicire, Professor (30225388)
Project Period (FY)	2005 – 2007
Keywords	Bone marrow stromal cells / organotopic hipocampal slice / 海馬切片培養
Research Abstract	Bone marrow stromal cells taken from EGFP transgenic mice were sorted by magnetic beads with surface markers of Sca-1 and Thy-1. The cells were then co-cultured on organotopic hippocampal slice or with neuronal cell feeder in dish. On hippocampus, both Sca-1 and Thy-1 positive cells showed 4- 8 folds higher potential to show neuron-like morphology than negative cells. In dish, negative cells fewly survived but each positive cells survived and showed neuron-like differentiation. In both culture condition, retinoic acid supplement accelerate differentiation. Differentiated Sca-1 and Thy-1 positive cells were immunohistochemically GFAP- and NeuN-negative but nestin-, neurofilament and NSE-positive. Neuron-like differentiation of bone marrow cells can be enhanced by selection using cell surface proteins.