2006 Fiscal Year Final Research Report Summary
Nano-particles which Contained Drug Induction into Tumor Cell by Electrochemotherapy and Sonoporation
| Project/Area Number |
17591559
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Orthopaedic surgery
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| Research Institution | Shinshu University |
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Principal Investigator |
TAKAHASHI Jun Shinshu University, University Hospital, Senior Assistant Professor, 医学部附属病院, 講師 (60345741)
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| Co-Investigator(Kenkyū-buntansha) |
NIKAIDO Toshio Toyama University, School of medicine, Professor, 医学部, 教授 (50180568)
WAKITANI Shigeyuki Osaka city University, School of medicine, Assistant Professor, 医学部, 講師 (70243243)
FURUTA Masakazu Osaka Prefecture University, School of science, Associate Professor, 医学部, 助教授 (40181458)
ISOBE Kenichi Shinshu University, University Hospital, Assistant Professor, 医学部附属病院, 助手 (80397314)
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| Project Period (FY) |
2005 – 2006
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| Keywords | nano-particle / sonoporation / drug delivery system / anti cancer drug |
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| Research Abstract |
1. Manufacture of nano-particles which contained an anti cancer drug
We made polypeptide [ (GVGVP) 25] besides the polypeptide Poly [ (GVGVP GVGVP GKGVP GVGVP GVGVP GVGVP)_<22>(GVGVP)] which made in last year and impregnated red dyes (Brebrich Scarlet Red) in the [ (GVGVP) 25] particle. Pigments were gradually discharged from them more than two weeks. We let 3. 5mg methotrexate fuse for the 30mg peptide. As a result, MTX was released more than minimum 3 days.
2 Nano-particles conjugated anticancer drug and anticancer drug induction into tumor cell by sonoporation and enhanced antitumor effects
We examined whether enhancement of the antitumor effect of sonoporation was feasible by using nano-particle of methotrexate component in mice Lewis lung carcinoma. The tumor bearing mice were divided into five groups : (1) no treatment (2) methotrexate treatment alone (3) nano-particle of methotrexate component treatment alone (4) methotrexate treatment followed by sonoporation(5)nano-particle of methotrexate component treatment followed by sonoporation. Sonoporation was applied to the tumors after intraperitoneal injection of drugs. Tumors of the mice were measured every 2 days after treatment. Differences in the rate of tumor volume increase between No. (4), (5) groups and other three groups were significant. As for No. (5) group in particularly, antitumor effect was enhanced. The methotrexate content of the tumor tissues from the sonoporation groups (No. (4), (5)) were higher than other groups. To confirm the promotion of methotrexate induction in vivo tumor cells by sonoporation, fluorescence conjugated MTX was administered into tumor cell. In the presence of sonoporation, fluorescence-positive cells were observed, while only a weakly positive area was observed in the absence of sonoporation.
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