2006 Fiscal Year Final Research Report Summary
Clarification of the roles of SDF-1 on mesenchymal stem cells in bone graft healing-toward a clinical application for the improvement of allograft healing-
| Project/Area Number |
17591564
|
|---|
| Research Category |
Grant-in-Aid for Scientific Research (C)
|
| Allocation Type | Single-year Grants |
|---|
| Section | 一般 |
|---|
| Research Field |
Orthopaedic surgery
|
|---|
| Research Institution | Kyoto University |
|---|
Principal Investigator |
ITO Hiromu Kyoto University, Graduate School of Medicine, Assistant Professor, 医学研究科, 助手 (70397537)
|
|---|
| Co-Investigator(Kenkyū-buntansha) |
NAKAMURA Takashi Kyoto University, Graduate School of Medicine, Professor, 医学研究科, 教授 (10201675)
TASHIRO Kei Kyoto Prefectural University of Medicine, Graduate School of Medicine, Professor, 医学研究科, 教授 (10263097)
|
|---|
| Project Period (FY) |
2005 – 2006
|
| Keywords | mesenchymal stem cell / bone transplantation / bone formation / SDF-1 |
|---|
| Research Abstract |
sDF-1 and CXCR4 were constitutively expressed by primary rBMSCs during osteoblastic and chondrocytic differentiation. The expression of SDF-1 mRNA was increased by 3.1 folds in the autograft healing at day 2 after surgery compared with that in the allograft model. Immunohistochemical analysis revealed the high expression of SDF-1 protein in the periosteum of the autografts. While the gain of function studies showed no remarkable increase in the bone formation, both the new bone formation around the autograft and the recruitment of MSCs were significantly inhibited by the administration of anti-SDF-1 antibody. SDF-1 upregulated the in vitro migration of rBMSCs in a dose dependent manner, and the effect was inhibited by TN 14003, an antagonist for CXCR4. These results indicate that SDF-1 has a chemotactic effect on BMSCs both in vitro and in vivo and plays a crucial role in the acute phase of normal bone repair. However, these results also suggest that SDF-1 may need to collaborate with other molecules to achieve a successful bone repair.
|
Research Products
(2 results)
