2007 Fiscal Year Final Research Report Summary
Axonal outgrowth after peripheral nervous system injury by cyclin-dependent kinase inhibitor ; INK4 family protein
| Project/Area Number |
17591567
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Orthopaedic surgery
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| Research Institution | Osaka University |
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Principal Investigator |
MURASE Tsuyoshi Osaka University, Graduate School of Medicine, Assistant Professor (50335361)
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| Co-Investigator(Kenkyū-buntansha) |
YOSHIKAWA Hideki Osaka University, Graduate School of Medicine, Professor (60191558)
MORITOMO Hisao Osaka University, Graduate School of Medicine, Assistant Professor (00332742)
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| Project Period (FY) |
2005 – 2007
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| Keywords | cyclin-dependent kinase inhibitor / peripheral nervous sytem / axonal outgrowth |
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| Research Abstract |
Cip/Kip family proteins (p21, p27, p57), cyclin-dependent kinase inhibitor, are known to inhibit Rho/Rho-kinase pathway, which inhibits neurite outgrowth. On the other hand, there is no report that INK4 family proteins (p15, p16, p18, p19), which is also cyclin-dependent kinase inhibitor, have an important roles to the peripheral nervous system except cell cycle. Therefore we started this project to investigate whether INK4 family proteins have good effects to the peripheral nervous system and contribute to the functional recovery after peripheral nervous system injury. Initially we performed cloning of INK4 family gene. Then we constructed the vectors with myc-tag at the N- or C- terminal. We investigated the intracellular localization of INK4 family proteins with HEK293 cells. All INK4 family proteins were expressed in the nucleus of HEK293 cells. INK4 family proteins were expressed in dorsal root ganglion neurons by Adenovirus with INK4 family gene. All INK4 family proteins were expressed in the nucleus of dorsal root ganglion neurons and did not influence axonal outgrowth, the number of neurite, and the number of neurite branching. p21 and p27 are known to be translocated from nucleus to cytoplasm by phosphorylation of threonine or serine and therefore we constructed the INK4 family vectors modified by phosphorylation of threonine and serin and investigated whether INK4 family protein was translocated from nucleus to cytoplasm by phosphorylation of threonine and serine. We did not observe the phenomenon of translocation from nucleus to cytoplasm and all INK4 family proteins were expressed in the nucleus.
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