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2007 Fiscal Year Final Research Report Summary

Axonal outgrowth after peripheral nervous system injury by cyclin-dependent kinase inhibitor ; INK4 family protein

Research Project

Project/Area Number 17591567
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Orthopaedic surgery
Research InstitutionOsaka University

Principal Investigator

MURASE Tsuyoshi  Osaka University, Graduate School of Medicine, Assistant Professor (50335361)

Co-Investigator(Kenkyū-buntansha) YOSHIKAWA Hideki  Osaka University, Graduate School of Medicine, Professor (60191558)
MORITOMO Hisao  Osaka University, Graduate School of Medicine, Assistant Professor (00332742)
Project Period (FY) 2005 – 2007
Keywordscyclin-dependent kinase inhibitor / peripheral nervous sytem / axonal outgrowth
Research Abstract

Cip/Kip family proteins (p21, p27, p57), cyclin-dependent kinase inhibitor, are known to inhibit Rho/Rho-kinase pathway, which inhibits neurite outgrowth. On the other hand, there is no report that INK4 family proteins (p15, p16, p18, p19), which is also cyclin-dependent kinase inhibitor, have an important roles to the peripheral nervous system except cell cycle. Therefore we started this project to investigate whether INK4 family proteins have good effects to the peripheral nervous system and contribute to the functional recovery after peripheral nervous system injury. Initially we performed cloning of INK4 family gene. Then we constructed the vectors with myc-tag at the N- or C- terminal. We investigated the intracellular localization of INK4 family proteins with HEK293 cells. All INK4 family proteins were expressed in the nucleus of HEK293 cells. INK4 family proteins were expressed in dorsal root ganglion neurons by Adenovirus with INK4 family gene. All INK4 family proteins were expressed in the nucleus of dorsal root ganglion neurons and did not influence axonal outgrowth, the number of neurite, and the number of neurite branching. p21 and p27 are known to be translocated from nucleus to cytoplasm by phosphorylation of threonine or serine and therefore we constructed the INK4 family vectors modified by phosphorylation of threonine and serin and investigated whether INK4 family protein was translocated from nucleus to cytoplasm by phosphorylation of threonine and serine. We did not observe the phenomenon of translocation from nucleus to cytoplasm and all INK4 family proteins were expressed in the nucleus.

  • Research Products

    (2 results)

All 2008

All Journal Article (2 results) (of which Peer Reviewed: 1 results)

  • [Journal Article] IL-1b promotes neurite outgrowth by deactivating RhoA via p38 MAPK pathway.2008

    • Author(s)
      Ko Temporin
    • Journal Title

      Biochem Biophys Res Commun. 365

      Pages: 375-380

    • Description
      「研究成果報告書概要(和文)」より
    • Peer Reviewed
  • [Journal Article] IL-1b promotes neurite outgrowth by deactivating RhoA via p38 MAPK pathway2008

    • Author(s)
      Ko, Temporin
    • Journal Title

      Biochem Biophys Res Commun 365

      Pages: 375-380

    • Description
      「研究成果報告書概要(欧文)」より

URL: 

Published: 2010-02-04  

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