Study about the effect of inhalation anesthetics on leukocyte adhesion and emigration after cerebral ischemia

2006 Fiscal Year Final Research Report Summary

• Project/Area Number: 17591611
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Anesthesiology/Resuscitation studies
• Research Institution: University of Tsukuba
• Principal Investigator: MIYABE Masayuki University of Tsukuba, Graduate School of Comprehensive Human Science, Associate Professor, 大学院人間総合科学研究科, 助教授 (60145589)
• Project Period (FY): 2005 – 2006
• Keywords: cerebral infarction / leukocyte / adhesion / sevoflurane

Research Abstract

Objective : Inflammatory response of leukocyte is one of the causes of cerebral injury after trauma, transplantation, or ischemia. This is speculated from the facts, elimination of leukocytes or administration of ICAM antibody, which prevents adhesion of leukocyte to vessel endothelial cell, decreases cerebral injury after ischemia. On the other hand, inhalation anesthetic sevoflurane accelerates leukocyte adhesion and emigration in mesenteric vein. If sevoflurane stimulate leukocyte adhesion and emigration in cerebral circulation, sevoflurane is not suitable for neurosurgry. On the other hand, sevofluran has cerebral protective effect against ischemia. And it is unknown that sevoflurane protect brain by preventing leukocyte adhesion and emigration during ischemia. Therefore the aim of this study was to investigate the effect of sevoflurane on the leukocyte adhesion and emigration after cerebral ischemia. Methods : Japanese white rabbits were anesthetized by intravenous pentobarbital and cranial window was made on the parietal bone. Pial venule, 50 μm diameter, was observed using microscope with video camera and recorded on personal computer. To visualize leukocytes, rhodamine 6G was administered. The number of rolling, adhesion, and emigration of leukocyts was counted. To make cerebral ischemia, carotid arteries were occluded bilaterally and mean arterial blood pressure (MABP) was maintained at 50 mmHg by bleeding. After 1-hour ischemia, reperfusion was performed by perfusion of carotid arteries and infusion of blood. After surgery the animals were divided into two groups, pentobarbital group or sevoflurane group. Results and Discussion : We could not obtain enough data to draw conclusion. I would like to continue research if sevoflurane would prevent leukocyte adhesion or emigration after cerebral ischemia and reperfusion.