2007 Fiscal Year Final Research Report Summary
N-cadherin expression is associated with bladder cancer progression and poor prognosis
| Project/Area Number |
17591664
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Urology
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| Research Institution | The University of Tokyo |
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Principal Investigator |
TOMITA Kyoichi The University of Tokyo, Hospital, associate professor (20272578)
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| Co-Investigator(Kenkyū-buntansha) |
KUME Haruki The University of Tokyo, Hospital, assistant professor (10272577)
KITAMURA Tadaichi The University of Tokyo, Hospital, Professor (70010551)
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| Project Period (FY) |
2005 – 2007
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| Keywords | bladder cancer / invasion / E-cadherin / N-cadherin / prognosis / confocal scanning laser microscopy / cadherin switching |
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| Research Abstract |
We investigated the expression of N-cadherin in cultured human bladder cancer cells and human bladder cancer specimens. We aim at establishing the pathological role of induced expression of N-cadherin in bladder cancer progression and show a correlation between expression of cadherins and clinical outcome. In 45 bladder cancer specimens of radical cystectomy or transurethral resection, double-label immunofluorescece staining of both E-and N-cadherin was performed, and patterns of these expressions were evaluated. We investigated to clarify the localization of N-cadherin expression using confocal scanning laser microscopy (CSLM). In cultured cancer cells, we did not confirm to change from E-cadherin to n-cadherin according to hepatocyte growth factor. Normal expression of E-cadherin was found in 20 cases (44.4%), and positive expression of N-cadherin (membranous pattern and/or dotted pattern) was found in 24 cases (53.3%). Aberrant expression of E-cadherin and positive expression of N-cadherin have a significant correlation with tumor stage. The ratio of 5 years survival rate was as follows:E-cadherin (normal 100%: aberrant 59.2%), and N-cadherin (positive 62.5%: negative 94.7%), respectively. There was a significant difference in survival between normal and aberrant expression of E-cadherin, or between positive and negative expression of N-cadherin. Bladder cancer cells invading stroma strongly showed positive N-cadherin expression by CSLM. In bladder cancer patients, there is a significant correlation between E-cadherin and N-cadherin, and pT stage. There is a significant correlation between aberrant expression of E-cadherin and positive expression of N-cadherin. Aberrant expression of E-cadherin and positive expression of N-cadherin show poor prognostic factors in survival of bladder cancer patients. Our data revealed N-cadherin expression may play an important role in bladder cancer invasion and metastasis.
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