2007 Fiscal Year Final Research Report Summary
Identification of Origin and Elucidation of Growth and Differetniation Mechanism of Satellite Cells in Human Urethral Rhabdosphincter
| Project/Area Number |
17591687
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Urology
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| Research Institution | Oita University |
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Principal Investigator |
MIMATA Hiromistu Oita University, Faculty of Medicine, Professor (60219714)
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| Co-Investigator(Kenkyū-buntansha) |
SATO Fuminori Oita University, Faculty of Medicine, Associate Professor (30305049)
HIRATA Yuji Oita University, Faculty of Medicine, Associate Professor (30295183)
MATSUBARA Takanori Oita University, Faculty of Medicine, Instructor (40315338)
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| Project Period (FY) |
2005 – 2007
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| Keywords | urethral rhabdosnhincter / growth factor / HGF / IGF-I / aoontosis / urinary incontinence / TNF-α / stern cell |
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| Research Abstract |
Objective : Urethral rhabdosphincter is the most important tissue for preservation of urinary continence, and its injury and/or decrease due to aging and prostate surgery induced stress urinary incontinence. This study was performed to clarify and culture satellite cells in human urethral rhabdosphincter for the elucidation of growth and differentiation mechanism and development of new therapeutic modality for urethral rhabdosphincter regeneration.
Materials and Method : Human urethral rhabdosphincter was obtained from patients who underwent radical prostatectomy or radical cystoprostatectomy. After the specimens were treated with collagenase and cultured, satellite cells in human urethral rhabdosphincter were isolated by MACS technique using anti-NCAM antibody conjugated with microbeads. Isolated satellite cells were confirmed as striated muscle origin by immunocytochemistry with anti-Myf-5 and anti-MyoD antibodies, and they were transfected with SV40 T antigen to obtain longevity.
Results and Conclusion : Satellite cells of human urethral rhabdosphincter produced HGF and IGF-I at mRNA and protein levels, which stimulated their growth. HGF and IGF-I activated MAPK and PI3-K signal transduction in satellite cells of human urethral rhabdosphincter, respectively.
Human urethral rhabdosphincter is reported to decrease due to apotosis with aging. In this study, TNF-α induced apoptosis by dose-dependent fashion in satellite cells of human urethral rhabdosphincter for the first time. Inhibitors of TNF-α may protect human urethral rhabdospincter from reduction with aging and be useful for the treatment and prevention of stress urinary incontinence in elderly.
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Research Products
(28 results)
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[Presentation] TNF-α induces apoptosis through receptor activation in human urethral rhabdosphincter2008
Author(s)
Mari, Hanada, Yasuhiro, Sumino, Yuji, Hirata, Mutsushi, Yamasaki, Fuminori, Sato, Hiromitsu, Mimata
Organizer
The 17th Japanese Society for Molecular and Cellular Urology
Place of Presentation
Tokyo
Year and Date
2008-02-16
Description
「研究成果報告書概要(欧文)」より
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