2006 Fiscal Year Final Research Report Summary
Methylation of DNA as a prognostic marker for bladder cancer
| Project/Area Number |
17591700
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Urology
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| Research Institution | Kyorin University |
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Principal Investigator |
OKEGAWA Takatsugu Kyorin University, School of Medicine, Associate Professor, 医学部, 助教授 (70306679)
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| Co-Investigator(Kenkyū-buntansha) |
NUTAHARA Kikuo Kyorin University, School of Medicine, Professor, 医学部, 教授 (00143470)
HIGASHIHARA Eiji Kyorin University, School of Medicine, Professor, 医学部, 教授 (00092312)
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| Project Period (FY) |
2005 – 2006
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| Keywords | bladder cancer / hypermethylation / FANCF gene |
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| Research Abstract |
PURPOSE : We investigate the extent of promoter hypermethylation in TCC throughout the urinary tract.
PATIENTS AND METHODS : Tissue was obtained from 120 patients (median followup, 38 months) with urothelial cancer. Analysis for methylation at FANCF, using methylation-sensitive polymerase chain reaction and bisulfite sequencing, was performed for each sample and compared with the tumor's clinicopathologic details, microsatellite instability status, and subsequent behavior.
RESULTS : Promoter methylation was present in 86% of urothelial cancer. In grade, methylation at FANCF indicated grade1 : 15%, grade2 : 28%, grade3 : 68%. In stage, methylation at FANCF indicated pTa : 12% pT1: 34%, pT2-4 : 57%. Methylation of FANCF was associated with advanced tumor stage (P =.0002) and higher tumor progression (P = 0.04.
CONCLUSION : Methylation occurs commonly in urinary tract tumors, may affect carcinogenic mechanisms, and is a prognostic marker and a potential therapeutic target.
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