2007 Fiscal Year Final Research Report Summary
Cytokines' role, especially G-CSF and IL-6, on human ovarian function and its clinical application
| Project/Area Number |
17591762
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Obstetrics and gynecology
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| Research Institution | Kanazawa Medical University |
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Principal Investigator |
MAKINODA Satoru Kanazawa Medical University, School of Medicine, Professor (80165688)
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| Co-Investigator(Kenkyū-buntansha) |
TOMCO Waseda Kanaawa Medical University, School of Medicine, Assistant Professor (40367488)
TOMIZAWA Hideki Kanaawa Medical University, School of Medicine, Assistant Professor (40319059)
FUJII Ryota Kanaawa Medical University, School of Medicine, Senior Assistant Professor (30298351)
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| Project Period (FY) |
2005 – 2007
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| Keywords | Ovarian function / luteal regression, cytokine / cytokine / G-CSF / IL-6 / Real-time quantitative RT-PCR / ovulation induction / clinical trial |
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| Research Abstract |
We have revealed the effects of cytokines, especially G-CSF and IL-6 on ovarian function as follows.
1) Cyclic changes of cytokines during the normal menstrual cycle
The cyclic changes of the cytokines▲(TNF-α, IL-1β, IL-6, GM-CSF, M-CSF) in serum that were already reported to be involved in the mechanism of ovulation and G-CSF were measured during the normal menstrual cycle. Only G-CSF showed a significant increase a few days before ovulation. Although it was not significant, IL-6 showed an increase during the late luteal phase.
2) Cyclic changes of IL-6 at the human follicular wall and its relation to the luteal regression
The expression of IL-6 mRNA at the human follicular wall increased significantly in the regressing corpus luteum. Moreover, increased IL-6 protein and receptor were observed in the follicular wall of the late luteal phase. These results will contribute to elucidate the mechanism of luteal insufficiency.
3) Clinical trial to use G-CSF as a supportive drug to ovulation induction
The following clinical trials were conducted to induce ovulation in the patients of luteinized unruptured follicle (LUF). The patients who showed LUF at the last Clomiphene-hCG cycle participated in this clinical trial. Ovulation induction using Clomiphene-hCG was performed as the last cycle. In addition, G-CSF 100 μg was administered 24-48 hours before hCG. The ovulation was successfully induced at the rate of 87.1% (54/62). Since the ovulation rate of the patients before this clinical trial was 53.5 %, the rate is significantly high (p<0.01).
Our results established a new method to induce ovulation using G-CSF.
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