• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to project page

2006 Fiscal Year Final Research Report Summary

The Stiffness Change of Conditioning Effect in Inner Hair Cells

Research Project

Project/Area Number 17591774
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Otorhinolaryngology
Research InstitutionTohoku University

Principal Investigator

YOSHIDA Naohiro  Tohoku University, GRADUATE SCHOOL OF MEDICINE, LECTURER, 大学院医学系研究科, 非常勤講師 (90291260)

Co-Investigator(Kenkyū-buntansha) WADA Hiroshi  Tohoku University, GRADUATE SCHOOL OF ENGINEERING, PROFESSOR, 大学院工学研究科, 教授 (30111264)
Project Period (FY) 2005 – 2006
Keywordsacoustic trauma / heat stress / hair cell
Research Abstract

Overexposure to intense sound damages the inner ear cells can lead to permanent threshold shift (PTS). Conditioning effect can protect the ear from acoustic injury. The mechanism of this conditioning effect has been still unknown.
In this research project, the mechanism of conditioning effect has been clarified by using 1) stiffness measurement by atomic force microscopy, 2) histological findings of f-actin and prestin by confocal microscope, 3) electrophysiological assay by DPOAE measurement. CBA strain mice were used in this project. The stiffness of outer hair cells has been elevated at 3, 6 hours after heat stress (41.5 degree, 15min). Furthermore, f-actin also has been elevated for 12 hours after heat stress. On the other hand, prestin which is the specific motor protein in outer hair cells, has not been elevated after heat stress. The enhancement of DPOAEs input-output function were observed in the same time, 3 to 6 hours after heat stress. PTS was protected dramatically at 6hours heat stress-trauma interval. These results would show the mechanism of conditioning effect by changing the stiffness with f-actin in inner hair cells.

  • Research Products

    (5 results)

All 2007 2006

All Journal Article (5 results)

  • [Journal Article] 音響外傷に関する最近の知見2007

    • Author(s)
      吉田 尚弘 他
    • Journal Title

      耳鼻咽喉科臨床 100(5)

      Pages: 323-331

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Recent findings in acoustic trauma2007

    • Author(s)
      Yoshida, N. et al.
    • Journal Title

      Jibiinnkoukarinsyou 100(5)

      Pages: 332-331

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Local mechanical properties of mouse outer hair cells : atomic force microscopic study.2006

    • Author(s)
      Murakoshi, M. et al.
    • Journal Title

      Auris Nasus Larynx 33(2)

      Pages: 149-157

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Effects of heat stress on Young' s modulus of outer hair cells in mice.2006

    • Author(s)
      Murakoshi, M. et al.
    • Journal Title

      Brain Research 1107(1)

      Pages: 121-130

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Effects of heat stress on Young's modulus of outer hair cells in mice.2006

    • Author(s)
      Murakoshi, M. et al.
    • Journal Title

      Brain Research 1107(1)

      Pages: 121-30

    • Description
      「研究成果報告書概要(欧文)」より

URL: 

Published: 2008-05-27  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi