2007 Fiscal Year Final Research Report Summary
Investigation of roles of nitric oxide in age-related cen aninemal hearing loss n Ring mock-out mice and its clinical application
| Project/Area Number |
17591784
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Otorhinolaryngology
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| Research Institution | Nagoya University |
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Principal Investigator |
TERANISHI Masaaki Nagoya University, Granduate School of Medicine, Associate Professor (20335037)
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| Co-Investigator(Kenkyū-buntansha) |
NAKASHIMA Tsutomu Nagoya University, Granduate School of Medicine, Professor (30180277)
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| Project Period (FY) |
2005 – 2007
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| Keywords | inner ear / aging / nitric oxide(NO) / oxidative stress |
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| Research Abstract |
Age-related hearing loss decreases quality of life of elderly people. Prevention and treatment of age-related hearing loss are important and urgent tasks. We investigated auditory function by auditory brain stem (ABR) responses, using C57BIJ8 mice as an experimental model of age-related hearing loss. We observed changes of nitric oxide and free-radical related substances in the inner ear using an immunohistcehemical method under a light microscope. ABE demonstrated that hearing levels of 7-8 month-old mice and 12 month-old mice were worse than those of 7 week-old mice. Hearing levels of 12 month-old mice were worse than those of 7-8 month-old mice. Expressions of inducible type NO synthase (NOS) and nitrotyrosine, a marker of peroxynitrite which is a strong radical, were enhanced in Corti organ, spiral ganglion cells and lateral wall of cochleae in 7-8 month-old mice, compared with 12 month-old mice. These results suggest that NO and oxidative stress is associated with cell damage and cell death of cochleae of aged mice. Hearing levels of mice with NOS gene deletion tend to be worse, as they grow old, compared with wild type, suggesting that NOS functions in a protective way for age-related heaving Ices. Active firm of Alit (phospho-Akt) influences NO production by activation of endothelial NOS (eNOS). Expression of Akt was observed in cochlea and vestibule in our experiments. This suggested that phospho-Akt is associated with survival signal in the inner eat.
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[Journal Article] Otoacoustic emissions, ear fullness and tinnitus in the recovery course of sudden deafness2008
Author(s)
Ishida, IM, Sugiura, M, Teranishi, M., Katayama, N, Nakashima, T
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Journal Title
Auris Nasus Larynx 35
Pages: 41-46
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] Visualization of endolymphatic hydrops in patients with Meniere's disease2007
Author(s)
Nakashima, T., Naganawam, S., Sugiura, M., Teranishi, M., Sone, M., Hayashi, H., Nakata, S., Katayama, N., Ishida, IM
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Journal Title
Laryngoscope 117
Pages: 415-420
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] Vestibular aqueduct in sudden sensorineural hearing loss2007
Author(s)
Sugiura, M., Naganawa, S., Ishida, IM., Teranishi, M., Nakata, S., Yoshida, T., Nakashima, T
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Journal Title
J Laryngol Otol (in press)
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] Blood flow to the promontory in cochlear otosclerosis2006
Author(s)
Nakashima, T., Sone, M., Fujii, H., Teranishi, M., Yamamoto, H., Otake, H., Sugiura, M., Naganawa, S
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Journal Title
Clin Otolaryngol 3
Pages: 110-115
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] Inner ear hemorrhage in systemic lupus erythematosus2006
Author(s)
Sugiura, M., Naganawa, S., Teranishi, M., Sato, E., Kojima, S., Nakashima, T
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Journal Title
Laryngoscope 116
Pages: 826-828
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] Distribution of pendrin in the organ of Corti of mice observed by electron immunomicroscopy2006
Author(s)
Yoshino, T., Sato, E., Nakashima, T., Teranishi, M., Yamamoto, H., Otake, H., Mizuno, T
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Journal Title
Eur Arch Otorhinolaryngol 263
Pages: 699-704
Description
「研究成果報告書概要(欧文)」より
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[Presentation] Visualization of endolymphatic hydrops in patients with inner ear diseases2007
Author(s)
Teranishi, M., Hayashi, H, Sugiura, M., Katayama, N., Nakata, S., Sone, M., Naganawa, S., Nakashima, T
Organizer
9th Japan-Taiwan Conference in Oto-Rhino-Laryngolgy, Head and Neck Surgery
Place of Presentation
Sendai (Japan)
Year and Date
2007-11-10
Description
「研究成果報告書概要(欧文)」より
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[Presentation] Intratympanic steroid application for idiopathic sudden sensorineural hearing loss as a salvage treatment2007
Author(s)
Teranishi, M., Yoshida, T., Hayashi, H., Iwata, T., Katayama, N., Nakata, S., Nakashima, T
Organizer
26th Politzer Society Meeting
Place of Presentation
Cleveland (USA)
Year and Date
2007-10-16
Description
「研究成果報告書概要(欧文)」より
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[Presentation] Endolymphatic and perilymphatic space imaging in patients with inner ear diseases2007
Author(s)
Nakashima, T., Teranishi, M., Sugiura, M., Sone, M., Hayashi, H., Naganawa, S
Organizer
26th Politzer Society Meeting
Place of Presentation
Cleveland (USA)
Year and Date
2007-10-14
Description
「研究成果報告書概要(欧文)」より
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[Presentation] Comparative study on sudden deafness by four nationwide epidemiological surveys in Japan2005
Author(s)
Teranishi, M., Katayama, N., Ishida, I.M., Uchida, Y., Tominaga, M., Toda, J., Nakashima, T
Organizer
25thPolitzer Society Meeting
Place of Presentation
Seoul (Korea)
Year and Date
2005-10-07
Description
「研究成果報告書概要(欧文)」より
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[Presentation] Cochlear blood flow in patients with sensorineural hearing loss2005
Author(s)
Nakashima, T., Sone, M., Teranishi, M., Yamamoto, H., Sugiura, M., Otake, H., Fujii, H
Organizer
25th Politzer Society Meeting
Place of Presentation
Seoul (Korea)
Year and Date
2005-10-07
Description
「研究成果報告書概要(欧文)」より
