網膜剥離後の細胞増殖におけるp27(KIP1)の役割

2005 Fiscal Year Annual Research Report

• Project/Area Number: 17591816
• Research Institution: Hokkaido University
• Principal Investigator: 吉田 和彦 北海道大学, 病院, 講師 (90281807)
• Keywords: p27(KIP1) / Muller細胞 / 網膜色素上皮 / 網膜剥離 / 細胞増殖

Research Abstract

これまで我々は野生型adultマウスにおいて、細胞周期関連蛋白の一つであるp27(KIP1)がMuller細胞および網膜色素上皮(RPE)細胞に発現していることを報告してきた(Yoshida et al.Anat Embryol 2004)。今回はマウス網膜剥離における細胞周期関連蛋白の発現と細胞増殖活性との関連について検討した。[材料と方法]生後12週のC57Bl6マウスを使用した。過去の報告を参照し網膜剥離を作製した(Yoshida et al.Graefes Arch Clin Exp Ophthalmol 2004)。剥離作製2日および4日後に還流固定し、眼球のパラフィン包埋標本を作製した。G1からS期への進行に関与するcyclinD1、細胞周期の進行停止に関与するp27(KIP1)について免疫組織化学的に検討した。細胞増殖活性の評価のためproliferating cell nuclear antigen(PCNA)の発現も検討した。[結果]正常ではp27(KIP1)はMuller細胞およびRPE細胞の核に発現されていたが、cyclinD1の発現はなかった。網膜剥離部のRPEでは剥離2日後からcyclinD1、PCNAの発現があり、一方p27(KIP1)の発現はなかった。Muller細胞ではp27(KIP1)の発現は2日後までみられたが、4日にはなかった。これとは対照的にPCNAは2日後で発現しておらず、4日後に発現していた。CyclinD1は2日から4日後にかけて発現していた。[結語]網膜剥離によりMuller細胞とRPE細胞は増殖活性を有し、これらはp27(KIP1)の分解、cyclinD1の発現を介する。

Research Products

• [Journal Article] Suppressive effects of astaxanthin against rat endotoxin-induceduveitis by inhibiting the NF-<kappa>B signaling pathway. (2006)
  • Author(s): Suzuki Y, Ohgami K, Shiratori K, Xue-Hai Jin, Ilieva B.I, Koyama Y, Yazawa K, Yoshida K, Kase S, Ohno S
  • Journal Title: Expeimental Eye Research 82 Pages: 275-281
• [Journal Article] Effects of blue honeysuckle(Lonicera caerulea L.) extract on lipopolysaccharide-induced inflammation in vitro and in vivo. (2006)
  • Author(s): Jin XH, Ohgami K, Shiratori K, Suzuki Y, Koyama Y, Yoshida K, Ilieva I, Tanaka T, Onoe K, Ohno S
  • Journal Title: Expeimental Eye Research 82 Pages: 860-867
• [Journal Article] Immunolocalization of cyclin D1 in the develoing lens of c-maf-/-mice. (2006)
  • Author(s): Kase S, Yoshida K, Sakai M, Ohgami K, Shiratori K, Kitaichi N, Suzuki Y, Harada T, Ohno S
  • Journal Title: Acta Histochem 107 Pages: 469-472
• [Journal Article] Expression of cdc2 and p27(KIP1) phosphorylation in mitotic cells of the human retinoblastoma. (2006)
  • Author(s): Kase S, Yoshida K, Ohgami K, Shiratori K, Suzuki Y, Nakayama K, Ohno S
  • Journal Title: Int J Mol Med 17 Pages: 465-468
• [Journal Article] Role of apoptosis signal-regulating kinase 1 in stress-induced neural cell apoptosis in vivo. (2006)
  • Author(s): Harada C, Nakamura K, Namekata K, Okumura A, Mitamura Y, Iizuka Y, Kashiwagi K, Yoshida K, Ohno S, Matsuzawa A, Tanaka K, Ichijo H, Harada T
  • Journal Title: American Journal of Pathology 168 Pages: 261-269
• [Journal Article] Expression of glutamine synthetase and cell proliferation in human idiopathic epiretinal membrane. (2006)
  • Author(s): Kase S, Saito W, Yokoi M, Yoshida K, Furudate N, Muramatsu M, Saito A, Kase M, Ohno S
  • Journal Title: British Journal of Ophthalmology 90 Pages: 96-98
• [Journal Article] Clinical features in tubulointerstitial nephritis and uveitis(TINU) syndrome. (2005)
  • Author(s): Goda C, Kotake S, Ichiishi A, Namba K, Kitaichi N, Ohno S
  • Journal Title: American Journal of Ophthalmology 140 Pages: 637-641
• [Journal Article] Long-term evaluation of visual field alteration in Lebers hereditary optic neuroathy with visual recovery. (2005)
  • Author(s): Naka M, Nitta T, Sato I, Shinmei Y, Chin S, Ohno S
  • Journal Title: Neuro Ophthalmol 29 Pages: 65-67
• [Journal Article] Phosphorylation of extracellular signal-regulated kinase and p27(KIP1) after retinal detachment. (2005)
  • Author(s): Kase S, Yoshida K, Harada T, Harada C, Namekata K, Suzuki Y, Ohgami K, Shiratori K, Nakayama K, Ohno S
  • Journal Title: Graefes Arch Clin Exp Ophthalmol 2 Pages: 1-7
• [Journal Article] Expression of thymidine phosphorylase in choroidal malignant melanoma associated with neovascular glaucoma. (2005)
  • Author(s): Kase S, Saito W, Yoshida K, Namba K, Osaki M, Ohgami K, Shiratori K, Kitaichi N, Adachi H, Ito H, Ohno S
  • Journal Title: Pathol Int 55 Pages: 569-573
• [Journal Article] Bilateral diffuse uveal melanocytic proliferation in a patient with cancer-associated retinopathy. (2005)
  • Author(s): Saito W, Kase S, Yoshida K, Ohguro H, Kinoshita H, Yokoi M, Iwaki H, Ohno S
  • Journal Title: American Journal of Ophthalmology 140 Pages: 942-945
• [Journal Article] Expression of p27(KIP1) and cell proliferation in human retina and retinoblastoma. (2005)
  • Author(s): Kase S, Yoshida K, Ohgami K, Shiratori K, Harada T, Ohno S
  • Journal Title: Anticancer Research 25 Pages: 3843-3846
• [Journal Article] Effects of fucoxanthin on lipopolysaccharide-induced inflammation in vitro and in vivo. (2005)
  • Author(s): Shiratori K, Ohgami K, Ilieva B.I, Xue-Hai Jin, Koyama Y, Miyashita K, Yoshida K, Kase S, Ohno S
  • Journal Title: Expeimental Eye Research 81 Pages: 422-428
• [Journal Article] The effects of naringin and naringenin on endotoxin-induced uveitis in rats. (2005)
  • Author(s): Shiratori K, Ohgami K, Ilieva B.I, Xue-Hai Jin, Yoshida K, Kase S, Ohno S
  • Journal Title: J Ocul Pharmacol Ther 21 Pages: 298-304
• [Journal Article] Phosphorylation of p27(KIP1) in the developing retina and retinoblastoma. (2005)
  • Author(s): Kase S, Yoshida K, Nakayama I K, Nakayama K, Ikeda H2, Harada T, Harada C, Ohgami K, Shiratori K, Ohno S
  • Journal Title: Int J Mol Med 16 Pages: 257-262
• [Journal Article] Comparative study of two sets of criteria for the diagnosis of Vogt-Koyanagi-Harada s disease : Comparative study of two sets of criteria for the diagnosis of Vogt-Koyanagi-Harada s disease. (2005)
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  • Journal Title: American Journal of Ophthalmology 139 Pages: 1080-1085
• [Journal Article] Disappearance of p27(KIP1) and increase in proliferation of the lens cells after extraction of most of the fiber cells of the lens. (2005)
  • Author(s): Kase S, Ikeda H, Harada T, Harada C, Imaki J, Ohgami K, Shiratori K, Nakayama K I., Nakayama K, Ohno S, Yoshida K
  • Journal Title: Current Eye Research 30 Pages: 437-442
• [Journal Article] A patient with sarcoidosis diagnosed by a biopsy of scleral nodules. (2005)
  • Author(s): Saito W, Kotake S, Ohno S
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• [Journal Article] Role of neurotrophin-4/5 in neural cell death during retinal development and ischemic retinal injury in vivo. (2005)
  • Author(s): Harada C, Harada T, Hun-Meng A.Q, Namekata K, Yoshida K, Ohno S, Tanaka K, L.F.Parada:
  • Journal Title: Invest Ophthalmol Vis Sci 46 Pages: 669-673
• [Journal Article] Anti-inflammatory effects of aronia extract on rat endotoxin-induced uveitis. (2005)
  • Author(s): Ohgami K, Ilieva B.I, Shiratori K, Koyama Y, Xue-Hai Jin, Yoshida K, Kase S, Kitaichi N, Suzuki Y, Tanaka T, Ohno S:
  • Journal Title: Invest Ophthalmol Vis Sci 46 Pages: 275-281
• [Journal Article] Usefulness of quantifying serum KL-6 levels in the follow-up of uveitic patients with sarcoidosis. (2005)
  • Author(s): Kitaichi N, Ariga T, Kase S, Yoshida K, Namba K, Ohno S
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