2007 Fiscal Year Final Research Report Summary
Camprehensive and development ct pharmacological treatment of retinopathy of prematurity
| Project/Area Number |
17591832
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Ophthalmology
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| Research Institution | Osaka University |
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Principal Investigator |
KUSAKA Shunji Osaka University, Graduate School of Medicine, Associate Professor (60260387)
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| Co-Investigator(Kenkyū-buntansha) |
OSHIMA Yusuke Osaka University, Graduate School of Medicine, Assistant Professor (20362717)
SAISHIN Yoshitsugu Osaka University, Graduate School of Medicine, Assistant Professor (30379193)
FUJIKADO Takashi Osaka University, Graduate School of Medicine, Professor (50243233)
TANO Yasuo Osaka University, Graduate School of Medicine, Professor (80093433)
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| Project Period (FY) |
2005 – 2007
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| Keywords | retinopathy of prematurity / VEGF / eDNA microarray / neovasucuralization / model mouse |
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| Research Abstract |
Background/aims : To investigate the correlation between the clinical course and gene expression pattern in murine oxygen-induced retinopathy (OIR), a common model of retinopathy of prematurity (ROP).
Methods: OIR was induced in C57BL/6N mice by placing postnatal day 7 (P7) pups in 75% oxygen for 5 days. The clinical course of OIR was evaluated on retinal flat-mounts with FITC-conjugated dextran perfusion from P12 to P21. The expression values of 94 genes, selected by microarray analysis, were determined daily from P12 through P21 by RT-PCR with TagMan (R) low-density array (TLDA) and analyzed by hierarchical clustering.
Results: TLDA cluster analysis showed the homology of gene expression pattern between P12 and P13, and between P16 and P17. Many genes associated with inflammation were up-regulated from P12 and P13, when the degrees of both central avascular area and central vasoconstriction were maximal, and the up-regulation of the genes continued to P21. Several representative genes associated with angiogenesis, e.g., vascular endothelial growth factor-A and angiopoietin-2, were most up-regulated at P16 and P17, when extraretinal neovascularization became most noticeable.
Conclusion: The gene expression pattern was well-correlated with the clinical presentation in murine OIR. These findings will contribute to understand the pathological condition involved in ROP.
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Research Products
(24 results)
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[Presentation] Efficacy of Intraocular Administration of Bevacizumab on Severe Retinopathy of Prematurity2007
Author(s)
Kusaka, S., Shima, C., Wakabayashi, T., Sato, T., Shimojyo, H
Organizer
ARVO meeting Fort Lauderdale
Place of Presentation
FL, USA
Year and Date
20070506-10
Description
「研究成果報告書概要(欧文)」より
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[Presentation] Effect of Electrical Stimulation on Cultured Retinal Muller Cells2007
Author(s)
Sato, T., Fujikado, T., Matsushita, K., Morimoto, T., Kanda, H., Harada, T., Kusaka, S., Tano, Y
Organizer
ARVO meeting Fort Lauderdale
Place of Presentation
FL, USA
Year and Date
20070506-10
Description
「研究成果報告書概要(欧文)」より
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[Presentation] Systemic and Ocular Adverse Events Following Intravitreal Injection of Bevacizumab2007
Author(s)
Shima, C., Sakaguchi, H., Kusaka, S., Kamei, M., Ikuno, Y., Sawa, M., Tsujikawa, M., Oshima, Y., Gomi, F., Tano, Y
Organizer
ARVO meeting Fort Lauderdale
Place of Presentation
FL, USA
Year and Date
20070506-10
Description
「研究成果報告書概要(欧文)」より
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[Presentation] Suprachoroidial-Transretinal Stimulation(STS) Artlflcal Vision System for Patients with Retinitis Pigmentosa2006
Author(s)
M., Kamei, T., Fujikado, H., Kanda, T., Morimoto, K., Nakauchi, H., Sakaguchi, Y., Ikuno, M., Ozawa, S., Kusaka, Y., Tano
Organizer
ARVO meeting Fort Lauderdale
Place of Presentation
FL, USA
Year and Date
20060430-0504
Description
「研究成果報告書概要(欧文)」より
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[Presentation] Clinical Studies of Intravitreal Injection of Bevacizumab(Avastin) for Rubeosis Associated With Proliferative Diabetic Retinopathy2006
Author(s)
H., Sakaguchi, Y., Oshima, M., Sawa, M., Tsujikawa, F., Gomi, Y., Ikuno, M., Kamei, S., Kusaka, Y., Tano
Organizer
ARVO meeting Fort Lauderdale
Place of Presentation
FL, USA
Year and Date
20060430-0504
Description
「研究成果報告書概要(欧文)」より
