2006 Fiscal Year Final Research Report Summary
Expression of Thymidine Phospholylase in Choroidal Neovascularization and inhibitors of TP suppress the fotmartion of Choroidal Neovascularization
| Project/Area Number |
17591847
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Ophthalmology
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| Research Institution | Fukushima Medical University |
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Principal Investigator |
IIDA Tomohiro Fukushima Medical University, Department of Ophthalmology, Professor, 医学部, 教授 (50241881)
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| Co-Investigator(Kenkyū-buntansha) |
TAKEBAYASHI Yuji Fukushima Medical University, Department of Ophthalmology, Assistant Professor, 医学部, 講師 (70321982)
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| Project Period (FY) |
2005 – 2006
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| Keywords | Thymidine Phosphorylase / Choroidal Neovascularization / immunohistochemical staining / age-related macular degeneration / idiopathic choroidal neovascularization / angioid streaks |
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| Research Abstract |
Thymidine phosphorylase (TP), an enzyme involved in pyrimidine nucleoside metabolism, is identical to platelet-derived endothelial cell growth factor, and has angiogenic activity in various tumor tissues. The purpose of this study was to determine whether TP is involved in the choroidal neovascularization, and thymidine phosphorylase inhibitor (TPI) suppresses choroidal neovascularization formation. Surgically removed choroidal neovascular membranes (CNVMs) were obtained at vitrectomy from three eyes with age-related macular degeneration (AMD), three eyes with angioid streaks (AS), three eyes with idiopathic choroidal neovascularization (ICNV), and two eyes with myopic choroidal neovascularization (mCNV). Light microscopic immunohistochemical staining and hematoxylin-eosin staining were performed to determine the cell type expressing TP. The intensity of staining was scored as follows : 0 is no staining ; 1 is slight staining ; 2 is moderate staining ; 3 is strong staining. All specimens had immunoreactivity of TP. The immunoreactivity was observed at following cell types in the CNVMs: macrophages, vascular cells, and retinal pigment epithelium (RPE) cells. Mean intensity score of all specimens were macrophages of 1.91±0.793, vascular cells of 1. 73± 0.962, and RPE of 1.36± 1.150. Mean intensity score of AMD is 2.33± 0.471, 1.33± 0.942, 2.0± 0.816, ICNV is 2.33±0.942, 2.0±0.816, 2.0±1.247, AS is 1.0±0, 2.0±0, 0.66±0.471, and mCNV is 2.0±0, 1.0±1.50, 0.50±0.50. TP produces strong immunoreactivity from Macrophages and vascular cells. Both AMD and ICNV show strong immunoreactivity for TP. We continuously investigate TPI suppresses the formation of CNVM in animal model.
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Research Products
(13 results)
