2006 Fiscal Year Final Research Report Summary
Role of scaffold stiffness dependent durotaxis on development of keloid
| Project/Area Number |
17591872
|
|---|
| Research Category |
Grant-in-Aid for Scientific Research (C)
|
| Allocation Type | Single-year Grants |
|---|
| Section | 一般 |
|---|
| Research Field |
Plastic surgery
|
|---|
| Research Institution | Chiba University |
|---|
Principal Investigator |
YOSHIMOTO Shinya Chiba University, Department of plastic and reconstructive surgery, Associate Professor, 大学院医学研究院, 助教授 (90220748)
|
|---|
| Co-Investigator(Kenkyū-buntansha) |
ICHINOSE Masaharu Chiba University, Department of plastic and reconstructive surgery, Professor, 大学院医学研究院, 教授 (90082156)
KITA Kazuko Chiba University, Department of Environmental Biochemistry, Instructor, 大学院医学研究院, 講師 (80302545)
NOMURA Jun Chiba University, Faculty of Education, Associate Professor, 教育学部, 助教授 (30252886)
SUGITA Katsuo Chiba University, Faculty of Education, Professor, 教育学部, 教授 (40211304)
|
|---|
| Project Period (FY) |
2005 – 2006
|
| Keywords | cell stretching / cDNA microarray / panel of antibody / rac1 / keloid / fibroblast / mechanical stress / stiffness of scaffold |
|---|
| Research Abstract |
Keloids are skin fibrotic conditions that can be caused by minor insults to skin, such as acne or ear piercing, or by severe injuries such as burns. Keloids are characterized by an overabundant deposition of extra-cellular matrix such as collagen, and their histologic morphology has a characteristic manner. Keloid occurs more often on chest, shoulder or elbow-joint etc. which is easily affected by mechanical stretch. So we consider that the response to mechanical stress of fibroblast derived from Keloid may be different from that of normal skin fibroblast. We analyzed the sensitivity to mechanical stress using cell stretch apparatus (NC-500) which has silicon chambers on which the fibroblasts are cultured. We investigated differential gene expression in response to cyclic cell stretching by gene chip array. We found that some genes of keloid fibroblasts encoding cellular skeleton, membrane proteins and cytokines were strongly changed compared to normal skin fibroblasts (We reported at The 14^<th> Research Council Meeting of Japan Society of Plastic and Reconstructive Surgery). Furthermore we found that the expression of rac1 protein is higher in keloid fibroblasts than in normal skin fibroblasts by panel of antibodies. In addition, we analyzed expression change of intracellular molecules after cell stretching. We cultured fibroblasts on elastic collagen coated polyacrylamide gel whose hardness is changeable and we found that cell shape and proliferation rate are variable depending on hardness of polyacrylamide gel.
|
