2007 Fiscal Year Final Research Report Summary
Research on immunoenhancing effect of hypertonic saline resuscitation of hemorrhagic shock model in mice
| Project/Area Number |
17591892
|
|---|
| Research Category |
Grant-in-Aid for Scientific Research (C)
|
| Allocation Type | Single-year Grants |
|---|
| Section | 一般 |
|---|
| Research Field |
Emergency medicine
|
|---|
| Research Institution | Kansai Medical University |
|---|
Principal Investigator |
MURAO Yoshinori Kansai Medical University, Department of Emergency and Critical Care Medicine, Associate professor (50211602)
|
|---|
| Project Period (FY) |
2005 – 2007
|
| Keywords | hemorrhagic shock / mouse / hypertonic saline / immunomodulatory effect / CD4^+CD25^+ regulatory T cell / γδ T cell |
|---|
| Research Abstract |
We examined effects of hypertonic saline resuscitation on CD4^+CD25^+ regulatory T cells and γδT cells after hemorrhagic shock and resuscitation in relation to apoptosis in mice. Methods: Male C57BL/6J mice 8 to 12 weeks old were used. Mice were anesthetized and blood was withdrawn until a mean arterial pressure of40±5mmHg was reached, and maintained for 60 min. Resuscitation was performed as follows: HS+SB; with hypertonic saline (4ml/Kg of 7.5% NaC1:HS) and shed blood (SB), 2LR+SB; with lactated Ringer' s solution (2 times the volume of the shed blood) and SB. Samples of thymus and spleen were harvested at 2,6,24 and 48h after resuscitation. CD4^+CD25^+ regulatory T cells and γδT cells were estimated using flow cytometry. Apoptosis was assessed with AnnexinV. Results: 1) Hypertonic saline resuscitation did not affect on CD4^+CD25^+ regulatory T cells and γ8T cells. 2) Regulatory T cells at 6h,24h and48h in spleen increased compared with controls. (P<0.05). 3) Apoptotic γδT cells and γδT cells both in spleen and thymus was not increased compared with controls. Discussion: Immunoenhancing effect of hypertonic saline resuscitation did not correlated with CD4^+CD25^+ regulatory T cells and γδT cells. Immunosuppressive condition after hemorrhagic shock and resuscitation may be related with CD4^+CD25^+ regulatory T cells.
|
