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2007 Fiscal Year Final Research Report Summary

Analysis for the regulation mechanisms of immuneresponse mediators in sepsis

Research Project

Project/Area Number 17591893
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Emergency medicine
Research InstitutionIwate Medical University

Principal Investigator

SATO Nobihiro  Iwate Medical University, School of Med., Dept of Critical Care Medicine, Lecturer (10244914)

Co-Investigator(Kenkyū-buntansha) MAESAWA Chihaya  Iwate Med. Univ., Dept of Pathol., Associate Prof. (10326647)
SUZUKI Yasushi  Iwate Med. Univ., Dept of Critical Care Med., Lecturer (90306019)
KOJIKA Masahiro  Iwate Med. Univ., Dept of Critical Care Med., Assistant (40347878)
Project Period (FY) 2005 – 2007
Keywordsmediators / proteome / cytokine / SIRS / TNF-α / IL / beads array
Research Abstract

It has been very difficult to indentify the unique secretory molecules out of numerous ones. However, recent advance of mass spectrometry technique makes it possible global analysis to resolve the problems. To investigate mediators controlling immune responses in sepsis, we performed two dimensional electrophoresis combined with Time-of-flight mass spectrometry (2D-TOF-MS) analysis. Serum samples of one hundred patients with sepsis were examined by TO-MS after informed consents were obtained. The results of 2D electrophoresis showed commonly 21 up-regulated and 16 down regulated spots, but there was no signals related with immune response. We additionally examined multiplex cytokines related with immune reposes using beads array and 2D-TOF-MS. These represented that ADAM17 (a disintegrin and metalloproteinase domain 17) related with TNFa secretion plays an important role in immune responses in hepatitis and sepsis. These results suggested that the analysis using the 2D-TOF-MS is not suit for detecting candidate molecules controlling immune responses because little amount of theses molecules.

  • Research Products

    (17 results)

All 2008 2006 2005

All Journal Article (14 results) (of which Peer Reviewed: 7 results) Presentation (3 results)

  • [Journal Article] Angiofensin II and epidermal growth factor receptor cross-talk mediated by a disintegrin and metalloprotease accelerates tumor cell proliferation of hepat ocellular carcinoma cell lines2008

    • Author(s)
      Itabashi H, Maesawa C, et. al.
    • Journal Title

      Hepatol Res 38

      Pages: 601-613

    • Description
      「研究成果報告書概要(和文)」より
    • Peer Reviewed
  • [Journal Article] Angiotensin II and epidermal growth factor receptor cross-talk mediated by a disintegrin and metalloprotease accelerates tumor cell proliferation of hepatocellular carcinoma cell lines2008

    • Author(s)
      Itabashi H, Maesawa C, Oikawa H, Kotani K, Sakurai E, Kato K, Komatsu H, Nitta H, Kawamura H, Wakabayashi G, Masuda, T
    • Journal Title

      Hepatol Res 38

      Pages: 601-613

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Interferon-gamma down-regulates expression of tumor necrosis factor-alpha c onverfing enzyme/a disintegrin and metailoproteinase 17 in activated hepatic stellate cells of rats2006

    • Author(s)
      Fujita T, Maesawa C, et. al.
    • Journal Title

      Int J Mol Med 17

      Pages: 605-616

    • Description
      「研究成果報告書概要(和文)」より
    • Peer Reviewed
  • [Journal Article] Endotoxin adsorption therapy for septic shock using polymyxin B-immobilized fi bers(PMX):evaluation by high-sensitivity endotoxin assay and measurement of the cvtokine oroduction capacity2006

    • Author(s)
      Kojika M, Sato N, et. al.
    • Journal Title

      Ther Apher Dial 10

      Pages: 12-18

    • Description
      「研究成果報告書概要(和文)」より
    • Peer Reviewed
  • [Journal Article] Interferon-gamma down-regulates expression of tumor necrosis factor-alpha converting enzyme/a disintegrin and metalloproteinase 17 in activated hepatic stellate cells of rats2006

    • Author(s)
      Fujita T, Maesawa C, Oikawa K, Nitta H, Wakabayashi G, Masuda, T
    • Journal Title

      Int J Mol Med 17

      Pages: 605-616

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Endotoxin adsorption therapy for septic shock using polymyxin B-immobilized fibers(PMX) : evaluation by high-sensitivity endotoxin assay and measurement of the cytokine production capacity2006

    • Author(s)
      Kojika M, Sato N, Yaegashi Y, Suzuki Y, Suzuki K, Nakae H, Endo, S
    • Journal Title

      Ther Apher Dial 10

      Pages: 12-18

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] A clinical study of liver abscesses at the Critical Care and Emergency Center of Iwate Medical University2005

    • Author(s)
      Fujino Y, Sato N, et. al.
    • Journal Title

      Nippon Shokakibyo Gakkai Zasshi 102

      Pages: 1153-1160

    • Description
      「研究成果報告書概要(和文)」より
    • Peer Reviewed
  • [Journal Article] Group IIA-soluble phospholipase A2 levels in patients with infections after eso phageal cancer surgery2005

    • Author(s)
      Ogawa M, Sato N, et. al.
    • Journal Title

      Surg Today 35

      Pages: 912-918

    • Description
      「研究成果報告書概要(和文)」より
    • Peer Reviewed
  • [Journal Article] Evaluation of a newly identified soluble CDt4 subtype as a marker for sepsis2005

    • Author(s)
      Yaegashi Y, Sato N, et. al.
    • Journal Title

      J Infect Chemother 11

      Pages: 234-238

    • Description
      「研究成果報告書概要(和文)」より
    • Peer Reviewed
  • [Journal Article] A preliminary study of the administration of carbapdnem antibiotics in sepsis patients on the basis of the administration time2005

    • Author(s)
      Kojika M, Sato N, et. al.
    • Journal Title

      Jpn J Antibiot 58

      Pages: 452-457

    • Description
      「研究成果報告書概要(和文)」より
    • Peer Reviewed
  • [Journal Article] A clinical study of liver abscesses at the Critical Care and Emergency Center of Iwate Medical University2005

    • Author(s)
      Fujino Y, Inoue Y, Onodera M, Yaegashi Y, Sato N, Endo S, Omori H, Suzuki, K
    • Journal Title

      Nippon Shokakibyo Gakkai Zasshi 102

      Pages: 1153-1160

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Group IIA-soluble phospholipase A2 levels in patients with infections after esophageal cancer surgery2005

    • Author(s)
      Ogawa M, Sato N, Endo S, Kojika M, Yaegashi Y, Kimura Y, Ikeda K, Iwaya, T
    • Journal Title

      Surg Today 35

      Pages: 912-918

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Evaluation of a newly identified soluble CD 14 subtype as a marker for sepsis2005

    • Author(s)
      Yaegashi Y, Shirakawa K, Sato N, Suzuki Y, Kojika M, Imai S, Takahashi G, Miyata M, Furusako S, Endo, S
    • Journal Title

      J Infect Chemother 11

      Pages: 234-238

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] [A preliminary study of the administration of carbapenem antibiotics in sepsis patients on the basis of the administration time]2005

    • Author(s)
      Kojika M, Sato N, Hakozaki M, Suzuki Y, Takahasi G, Endo S, Suzuki K, Wakabayasi, G
    • Journal Title

      Jpn J Antibiot 58

      Pages: 452-457

    • Description
      「研究成果報告書概要(欧文)」より
  • [Presentation] 腹重症敗血症における血液浄化法2006

    • Author(s)
      佐藤 信博
    • Organizer
      第13回外科侵襲とサイトカイン研究会
    • Place of Presentation
      宇部
    • Year and Date
      20061200
    • Description
      「研究成果報告書概要(和文)」より
  • [Presentation] 腹膜炎術後重痒敗血症に対する急性血液浄化法の検討2006

    • Author(s)
      佐藤 信博
    • Organizer
      第61回日本消化器外科学会定期学術集会
    • Place of Presentation
      横浜
    • Year and Date
      20060700
    • Description
      「研究成果報告書概要(和文)」より
  • [Presentation] 腹膜炎術後の経口摂取過程における経腸栄養補助療法の有用性2005

    • Author(s)
      佐藤 信博
    • Organizer
      第9回CCM輸液、栄養研究会
    • Place of Presentation
      東京
    • Year and Date
      20050500
    • Description
      「研究成果報告書概要(和文)」より

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Published: 2010-02-04  

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