2007 Fiscal Year Final Research Report Summary
Microarray and proteomics analysis on odontogenesis-related-genes in prenatal mouse dental papillae
Project/Area Number |
17591926
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Research Category |
Grant-in-Aid for Scientific Research (C)
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Allocation Type | Single-year Grants |
Section | 一般 |
Research Field |
Morphological basic dentistry
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Research Institution | Tokyo Dental College |
Principal Investigator |
MURAMATSU Takashi Tokyo Dental College, Department of Pathology, Assistant Professor (00276982)
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Co-Investigator(Kenkyū-buntansha) |
YAMAMOTO Hitoshi Nihon University, School of Dentistry at Matsudo, Department of Histology, Assistant Professor (80265165)
KOHNO Yohko Showa University, School of Dentistry, Department of Oral Pathology, Associate Professor (40195681)
SHIMONO Masaki Tokyo Dental College, Department of Pathology, Professor (00085771)
MURAMATSU Takashi Tokyo Dental College, Department of Pathology, Assistant Professor (00276982)
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Project Period (FY) |
2005 – 2007
|
Keywords | odontogenesis / tooth germ / dental ID anilla / microarrav / proteomics / Admts4 / Aldh1a2 / PlexinC1 |
Research Abstract |
Important factors for involved in odontogenesis in mouse dental papillae have been reported to disappear between the pre- and post-natal stages of development. Therefore, we hypothesized that the genes involved in odontogenesis in dental papillae might be down-regulated after birth. Our goal was to identify those which genes were down-regulated genes by microarray analysis. Dental papillae were dissected isolated from embryonic of 16-day and 18-day (E16, E18), and post-natal 3- and 10-day-old (P3, P10) murine mice first mandibular molar germs. The highest number of down-regulated genes (2924) was found at E18 and P3 Down-regulation of Adamts4 and Aldhala2 was found at both E16 and E18, and expression of Plxncl showed a reduction at E18 and P3. Investigation of expression of Adamts4, Aldhla2 and Plxncl was investigated by quantitative RT-PCR which showed revealed a reduction of in their expression after birth (Adamts4; 1/3, Aldhla2; 1/13, Plxncl; 1/3). These results suggest that three of the genes selected identified by the microarray analysis - Adamts4, Aldhla2, and Plxncl - are involved in odontogenesisin dental papillae.
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Research Products
(110 results)
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[Journal Article] Claudin rather than occludin is essential for differentiation in rat incisor odontoblasts2008
Author(s)
Hoshino, M, Hashimoto, S, Muramatsu, T, Matsuki, M, Ogiuchi, H, Shimono, M
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Journal Title
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] Temporospatial tissue interactions regulating the regeneration of the enamel knot in the developing mouse tooth2007
Author(s)
Sung-Won, Cho, Jae-Young, Kim, Jong-Min, Lee, Eun-Jung, Kim, Hyun-A, Lee, Hitoshi, Yamamoto, Han-Sung, Jung
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Journal Title
Differentiation 75(2)
Pages: 158-165
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] Hypermethylation of CpG islands in p16 as a prognostic factor for diffuse large B-cell lymphoma in a high-risk group2006
Author(s)
Shiozawa E, Takimoto M, Makino R, Adachi D, Saito B, Yamochi-Onizuka T, Shimozuma J, Maeda T, Kohno Y, Kawakami K, Nakamaki T, Tomoyasu S, Shiokawa A, Ota H
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Journal Title
Leukemia Research 30
Pages: 859-867
Description
「研究成果報告書概要(和文)」より
Peer Reviewed
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[Journal Article] Ota H Hypermethylation of CpG islands in p16 as a prognostic factor for diffuse large B-cell lymphoma in a high-risk group2006
Author(s)
Shiozawa, E, Takimoto, M, Makino, R, Adachi, D, Saito, B, Yamochi-Onizuka, T, Shimozuma, J, Maeda, T, Kohno, Y, Kawakami, K, Nakamaki, T, Tomoyasu, S, Shiokawa, A
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Journal Title
Leuk Res 30(7)
Pages: 859-67
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] The size and shape of dentine crystallites2005
Author(s)
Hideki Chisaka, Atsushi Ohkubo, Tomohisa Honda, Kazumasa Hoshino, Rumi Yokota, Kunihiro Suzuki, Hitoshi Yamamoto, Toshiaki Sakae, Yukishige Kozawa
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Journal Title
Scientific Journal of Japan Institute for Advanced Dentistry 11
Pages: 63-66
Description
「研究成果報告書概要(和文)」より
Peer Reviewed
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[Journal Article] Current Trends2005
Author(s)
Yukishige Kozawa, Rumi Yokota, Yoshinori Hanaizumi, Hideki Chisaka, Hitoshi Yamamoto, Kunihiro Suzuki
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Journal Title
Dental Morphology, University of Lodz Press
Pages: 391-394
Description
「研究成果報告書概要(和文)」より
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[Journal Article] Current Trends2005
Author(s)
Hitoshi Yamamoto, Jinglei Cai, Rumi Yokota, Kunihiro Suzuki, Hideki Chisaka, Han-Sung Jung, Yukishige Kozawa
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Journal Title
Dental Morphology, University of Lodz Press
Pages: 549-553
Description
「研究成果報告書概要(和文)」より
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[Journal Article] Reduction of osteocalcin expression in aged human dental pulp2005
Author(s)
Muramatsu, T, Hamano, H, Ogami, K, Ohta, K, Inoue, T, Shimono, M
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Journal Title
Int Endod 38(11)
Pages: 817-821
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] Odontoblast as a sensory receptor cell? : expression of TRPV1(VR-1) channels2005
Author(s)
Okumura, R, Shima, K, Muramatsu, T, Nakagawa, K, Shimono, M, Magloire, H, Suzuki, T, Shibukawa, Y
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Journal Title
Arch Histol Cytol 68(4)
Pages: 251-257
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] Microarray analysis on odontogenesis-related-genes in mouse dental papillae2005
Author(s)
Muramatsu, T, Sasaki, H, Yamamoto, H, Kohno, Y, Cho, SW, Jung, HS, Shimono, M
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Journal Title
J Hard Tissue Biology 14(2)
Pages: 180-182
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] Uni-axial stretching regulates intracellular localization of Hic-5 expressed in smooth-muscle cells in vivo2005
Author(s)
Kim-Kanayama, J, Suzuki, W, Ichikawa, K, Ohki, T, Kohno, Y, Sata, M, Nose, K, Shibanuma, M
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Journal Title
J Cell Sci 118
Pages: 937-949
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] Studies on the enamel structure of transplanted tooth germ2005
Author(s)
Hitoshi, Yamamoto, Jinglei, Cai, Kunihiro, Suzuki, Rumi, Yokota, Hideki, Chisaka, Toshiro, Sakae, Han-Sung, Jung, Yukishige, Kozawa
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Journal Title
Journal of Hard Tissue Biology 14(2)
Pages: 218-220
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] Immunohistochemical localization for manganese and copper, zinc superoxide dismutase in secretory ameloblasts of rat incisor2005
Author(s)
Hitoshi, Yamamoto, Rumi, Yokota, Kunihiro, Suzuki, Yuka, Iwasa, Hideki, Chisaka, Yukishige, Kozawa
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Journal Title
Journal of Comparative Development of Tooth Enamel 9
Pages: 39-42
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] Can the information from epithelium to mesenchvme reset using a reaeeregation system?2005
Author(s)
Hitoshi, Yamamoto, Eun-Jung, Kim, Sung-Won, Cho, Rumi, Yokota, Kunihiro, Suzuki, Yuka, Iwasa, Hideki, Chisaka, Han-Sung, Jung, Yukishige, Kozawa
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Journal Title
Journal of Comparative Development of Tooth Enamel 9
Pages: 28-30
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] On the dentine structure evolution with the correlation of enamel2005
Author(s)
Yukishige, Kozawa, Tetsuo, Terajima, Kunihiro, Suzuki, Hitoshi, Yamamoto, Rumi, Yokota, Hideki, Chisaka, Kazumasa, Hoshino, Tomohisa, Honda
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Journal Title
Journal of Comparative Development of Tooth Enamel 9
Pages: 19-27
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] Immunohistological study on the ameloblasts `grouping' and 'dancing' forming of enamel structure2005
Author(s)
Rumi, Yokota, Yoshinori, Hanaizumi, Kunihiro, Suzuki, Hideki, Chisaka, Hitoshi, Yamamoto, Toshiro, Sakae, Tsutomu, Hiraga, Yukishige, Kozawa
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Journal Title
Nihon Univ. J. Oral Sci 31(2)(in Japanese)
Pages: 77-94
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] The size and shape of dentine crystallites2005
Author(s)
Hideki, Chisaka, Atsushi, Ohkubo, Tomohisa, Honda, Kazumasa, Hoshino, Rumi, Yokota, Kunihiro, Suzuki, Hitoshi, Yamamoto, Toshiaki, Sakae, Yukishige, Kozawa
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Journal Title
Scientific Journal of Japan Institute for Advanced Dentistry 11(2)
Pages: 63-66
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] Origin and evolution of cementum as tooth attachment complex2005
Author(s)
Yukishige, Kozawa, Hideki, Chisaka, Yuka, Iwasa, Rumi, Yokota, Kunihiro, Suzuki, Hitoshi, Yamamoto
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Journal Title
Journal of Oral Biosciences 47(1)
Pages: 25-32
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] Characteristic tissue interaction of the diastema region in mice2005
Author(s)
Hitoshi, Yamamoto, Sung-Won, Cho, Soo-Jin, Song, Heui-Jung, Hwang, Min-Jung, Lee, Jae-Young, Kim, Han-Sung, Jung
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Journal Title
Archives of Oral Biology 50(2)
Pages: 189-198
Description
「研究成果報告書概要(欧文)」より
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[Presentation] 歯根の形態と分化2008
Author(s)
小澤 幸重, 鈴木 久仁博, 山本 仁, 横田 ルミ, 新美 寿英, 阿部 達彦, 山下 靖雄, 大島 勇人
Organizer
第113回日本解剖学会
Place of Presentation
大分市
Year and Date
2008-03-27
Description
「研究成果報告書概要(和文)」より
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[Presentation] ヒトの歯の形態形成要因の検討2007
Author(s)
小澤 幸重, 鄭 翰聖, 大島 勇人, 横田 ルミ, 山本 仁, 鈴木 久仁博, 寒河江 登志朗
Organizer
第49回歯科基礎医学会
Place of Presentation
札幌市
Year and Date
20070829-31
Description
「研究成果報告書概要(和文)」より
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[Presentation] Directional Ca^<2+> transport via sodium/calcium exchangers,NCX3,in rat odontoblasts during dentinogenesis2007
Author(s)
Shibukawa Y, Okumura R, Yamamoto T, Muramatsu T, Matsuda T, Baba A, Nakagawa K, Shimono M, Suzuki K, Schnetkamp PPM.
Organizer
第84回日本生理学学会
Place of Presentation
大阪市
Year and Date
20070320-22
Description
「研究成果報告書概要(和文)」より
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[Presentation] Ca^<2+> transporting/signaling mechanisms mediated by sodium/calcium exchangers in rat ameloblasts2007
Author(s)
Okumura, R, Suzuki, K, Muramatsu, T, Nakagawa, K, Shimono, M, Schnetkamp, P PM, Shibukawa, Y
Organizer
Journal of Physiological Sciences
Year and Date
20070000
Description
「研究成果報告書概要(欧文)」より
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[Presentation] 移植歯胚の形態形成に関する研究2007
Author(s)
樋口 由佳, 山本 仁, 鈴木 久仁博, 小澤 幸重
Organizer
平成19年度日本大学口腔科学会学生会員ボスター発表会
Place of Presentation
松戸市
Year and Date
2007-11-10
Description
「研究成果報告書概要(和文)」より
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[Presentation] 歯の形態形成制御機構に関する問題点2007
Author(s)
小澤 幸重, 土門 卓文, 齋藤 正寛, 脇坂 聡, Herve Lesot, 山本 仁, 大島 勇人, 原田 英光
Organizer
平成15年度選定学術フロンティア推進事業(継続分)年次報告会「加齢に伴う口腔機能の発達と維持機構の解明」
Place of Presentation
日本大学松戸歯学部 松戸市
Year and Date
2007-02-17
Description
「研究成果報告書概要(和文)」より
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[Presentation] Tooth morphogenesis of transplanted tooth germs and reaggregated tooth germs2007
Author(s)
Hitoshi, YAMAMOTO, Yuka, HIGUCHI, Jinglei, CAI, Han-Sung, JUNG, Kunihiro, SUZUKI, Rum, i YOKOTA-OHTA, Yukishige, KOZAWA
Organizer
Frontier meeting
Place of Presentation
Tokyo
Year and Date
2007-02-08
Description
「研究成果報告書概要(欧文)」より
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[Presentation] 歯の形態形成要因2006
Author(s)
小澤 幸重, 千坂 英輝, 横田 ルミ, 山本 仁, 鈴木 久仁博, 寒河江 登志朗, 大島 勇人, 鄭 翰聖
Organizer
第48回歯科基礎医学会学術大会, 鶴見
Place of Presentation
鶴見大学歯学部 横浜市
Year and Date
20060922-23
Description
「研究成果報告書概要(和文)」より
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[Presentation] Microarray analysis of pre- and postnatal murine dental papilla2006
Author(s)
Sasaki, H, Muramatsu, T, Jung, HS, Hashimoto, S, Shimono, M
Organizer
The 35th annual meeting & exhibition of the AADR
Place of Presentation
Orlando, Florida, USA
Year and Date
20060908-11
Description
「研究成果報告書概要(欧文)」より
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[Presentation] Ca^<2+> transporting/signaling mechanisms mediated by sodium/calcium exchangers inrat ameloblasts2006
Author(s)
Okumura R, Shima K, Muramatsu T, Nakagawa K, Shimono M, Suzuki T, Suzuki K, Magloire H, Schnetkamp PPM, Shibukawa Y
Organizer
84th International Association for Dental Research(IADR)
Place of Presentation
Brisbane,Australia
Year and Date
20060628-0701
Description
「研究成果報告書概要(和文)」より
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[Presentation] 歯の形態形成は顎の成長と深く関連する2006
Author(s)
小澤 幸重, 蔡 景蕾, 鄭 翰聖, 大島 勇人, 千坂 英輝, 横田 ルミ, 山本 仁, 鈴木 久仁博, 寒河江 登志朗
Organizer
第112回日本解剖学会
Place of Presentation
大阪市
Year and Date
20060326-29
Description
「研究成果報告書概要(和文)」より
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-
-
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[Presentation] 歯性間葉組織がもつ歯の潜在的形成能について2006
Author(s)
山本 仁, 脇坂 聡, 土門 卓文, 斎藤 正寛, 今井 元, Herve Lesot, 小澤 幸重
Organizer
平成15年度選定学術フロンティア推進事業(継続分)中間報告会「加齢に伴う口腔機能の発達と維持機構の解明」
Place of Presentation
日本大学松戸歯学部 松戸市
Year and Date
2006-02-25
Description
「研究成果報告書概要(和文)」より
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[Presentation] 歯の起源と形態形成2006
Author(s)
小澤 幸重, 土門 卓文, 斎藤 正寛, 脇坂 聡, Herve Lesot, 山本 仁, 大島 勇人, 原田 英光
Organizer
平成15年度選定学術フロンティア推進事業(継続分)中間報告会「加齢に伴う口腔機能の発達と維持機構の解明」
Place of Presentation
日本大学松戸歯学部松戸市
Year and Date
2006-02-25
Description
「研究成果報告書概要(和文)」より
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[Presentation] 象牙質の結晶形成2005
Author(s)
千坂 英輝, 大久保 厚司, 本田 知久, 星野 和正, 横田 ルミ, 鈴木 久仁博, 山本 仁, 寒河江 登志朗, 小澤 幸重
Organizer
第47回歯科基礎医学会学術大会
Place of Presentation
仙台市
Year and Date
20050929-30
Description
「研究成果報告書概要(和文)」より
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[Presentation] エナメル質の結晶形成と配列に関する試論2005
Author(s)
小澤 幸重, 千坂 英輝, 寒河江 登志朗, 大久保 厚司, 鈴木 久仁博, 山本 仁, 横田 ルミ
Organizer
第47回歯科基礎医学会学術大会
Place of Presentation
仙台市
Year and Date
20050929-30
Description
「研究成果報告書概要(和文)」より
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[Presentation] Expression of tight junction-associated proteins between osontoblasts in rat incisors2005
Author(s)
Hoshimo, M, Shibukawa, Y, Okumura, R, Matsuki, M, Muramatsu, T, Ogiuchi, H, Hashimoto, S, Shimono, M
Organizer
Dentin/Pulp Complex Meeting 2005
Place of Presentation
Duesseldorf. Germany
Year and Date
20050918-20
Description
「研究成果報告書概要(欧文)」より
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[Presentation] Microarray analysis on odontogenesis related genes in mouse dental papilla2005
Author(s)
Muramatsu, T, Sasaki, H, Yamamoto H, Kohno, Y, Cho, SW, Jung, HS, Shimono, M
Organizer
Symposium of Maxillofacial & Oral Regenerative Biology in OKAYAMA 2005
Place of Presentation
Okayama Japan
Year and Date
20050917-20
Description
「研究成果報告書概要(欧文)」より
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[Presentation] FEL ablation effects on human dental enamel and dentin2005
Author(s)
Sakae, T, Sato, Y, Okada, H, Yamamoto, H, Suzuki, K, Yamamoto, H, Kozawa, Y, Sato, I
Organizer
3rd Annual meeting of Japanese Association for Dental Research
Place of Presentation
Okayama Japan
Year and Date
20050917-20
Description
「研究成果報告書概要(欧文)」より
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[Presentation] Studies on the potential ability for tooth formation of the dental mesenchyme2005
Author(s)
Hitoshi, Yamamoto, Sung-Won, Cho, Eun-Jung, Kim, Jinglei, Chai, Jae-Young, Kim, Han-Sung, Jung, Yukishige, Kozawa
Organizer
Symposium of Maxillofacial & Oral Regenerative Biology in OKAYAMA 2005
Place of Presentation
Okayama Japan
Year and Date
20050917-20
Description
「研究成果報告書概要(欧文)」より
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[Presentation] Enamel structure of the transplanted tooth germ2005
Author(s)
Hitoshi, Yamamoto, Jinglei, Chai, Rumi, Yokota, Kunihiro, Suzuki, Hideki, Chisaka, Han-Sung, Jung, Yukishige, Kozawa
Organizer
13th Dental Morphology
Place of Presentation
Poland(Lodz)
Year and Date
20050824-27
Description
「研究成果報告書概要(欧文)」より
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[Presentation] Immunohistological study on the ameloblast `grouping and dancing' forming enamel structures and it evolution2005
Author(s)
Yukishige, Kozawa, Rumi, Yokota, Yoshinori, Hanaizumi, Kunihiro, Suzuki, Hideki, Chisaka, Hitoshi, Yamamoto, Minoru, Wakita
Organizer
13th Dental0 Morphology
Place of Presentation
Poland(Lodz)
Year and Date
20050824-27
Description
「研究成果報告書概要(欧文)」より
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[Presentation] Ca2+ transporting/signaling mechanisms mediated by sodium/calcium exchangers inrat ameloblasts2005
Author(s)
Okumura, R, Shima, K, Muramatsu, T, Nakagawa, K, Shimono, M, Suzuki, T, Suzuki, K, Magloire, H, Schnetkamp, PPM, Shibukawa, Y
Organizer
Japan physiology meeting
Place of Presentation
Osaka
Year and Date
20050320-22
Description
「研究成果報告書概要(欧文)」より
-
-
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[Book] 歯の発生2006
Author(s)
山本 仁(上田 実監修, 本田 雅規編著)
Total Pages
174-182
Publisher
真興交易(株)医書出版部
Description
「研究成果報告書概要(和文)」より
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[Book] Current TrendsDental Morphology2005
Author(s)
Yukishige Kozawa, Rumi Yokota, Yoshinori Hanaizumi, Hideki Chisaka, Hitoshi Yamamoto, Kunihiro Suzuki
Total Pages
391-394
Publisher
University of Lodz Press
Description
「研究成果報告書概要(和文)」より
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[Book] Current TrendsDental Morphology2005
Author(s)
Hitoshi Yamamoto, Jinglei Cai, Rumi Yokota, Kunihiro Suzuki, Hideki Chisaka, Han-Sung Jung, Yukishige Kozawa
Total Pages
549-553
Publisher
University of Lodz Press
Description
「研究成果報告書概要(和文)」より