2007 Fiscal Year Final Research Report Summary
Study of glycosylation mechanism and virulence of glycoproteins produced by streptococci
| Project/Area Number |
17591928
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Morphological basic dentistry
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| Research Institution | The Nippon Dental University |
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Principal Investigator |
KONISHI Kiyoshi The Nippon Dental University, Life Dentistry at Tokyo, professor (20178289)
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| Co-Investigator(Kenkyū-buntansha) |
TAKAHASHI Yukihiro The Nippon Dental University, Life Dentistry at Tokyo, associate professor (00281436)
YAJIMA Ayako The Nippon Dental University, Life Dentistry at Tokyo, assistant professor (00287773)
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| Project Period (FY) |
2005 – 2007
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| Keywords | infectious disease / bacteria / glycoprotein / streptococcus |
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| Research Abstract |
Streptococcus gordonii on the human tooth surface is associated with the formation of dental plaque and infectious endocarditis. We have previously reported that sialic acid-binding adhesin (Hsa) of this bacterium is a N-acetyl-glycosamine containing-glycoprotein, in which glycosylated region is Ser-rich repeat domain of Hsa, and is bound to glycol with O-glycosylation manner. An insertional mutation in hsa, the gene encoding Hsa, resulted in a significant reduction of the infection rate of the organism and an inflammatory reaction in the rat aortic valve with experimental endocarditis, suggesting that the Hsa contributes to the infectivity of the organism for heart valves. The contribution of the agglutination of platelet and erythrocyte on infectious endocarditis is estimated. We have 〓viously indicated that the receptor of platelet to Hsa of this bacterium is two kind of intrinsic membrane asialo-glycoprotein. We found that the interaction between Hsa and α2-3-linked sialic acid con
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tained by erythrocyte membrane proteins. We also confirmed that erythrocyte surface sialoglycoproteins, glycophorin A and band 3, are the receptor for Hsa. For finding the gene related to the glycosylation of Hsa, we have screened the transposon-mutation library of S. gordonii, and we identified the glmM gene encoding the phosphoglucosamine mutase which is concerned of the synthesis of cell wall in other bacteria. Insertional glmM mutant of this bacterium did not produce GlmM, and had a growth rate of about half of the wild type. Morphological analysis indicated that the glmM mutation causes elongation of the streptococcal chains, enlargement of bacterial cells, and increased roughness of the cell surface. Furthermore, glmM mutation reduces biofilm formation and increases sensitivity to penicillin relative to wild type. These results suggested that mutation in glmM appears to influence the phenotype relating to the peptidoglycan synthesis. We are now studying the relationship the function of GlmM and glycosylation of Hsa. Less
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[Journal Article] Hsa, an adhesin of Streptococcus gordonii DL1, binds to α2, 3-linked sialic acid on glycophorin A of the erythrocyte membrane2008
Author(s)
Ayako, Yajima, Yumiko, Urano-Tashiro, Kisaki, Shimazu, Eizo, Takashima, Yukihiro, Takahashi, Kiyoshi, Konishi
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Journal Title
Microbiol. Immunol 52
Pages: 69-77
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] Identification of the Streptococcus gordonii glmM gene encoding phosphoglucosamine mutase and its role in bacterial cell morphology, biofilm formation and sensitivity to antibiotics2008
Author(s)
Kisaki, Shimazu, Yukihiro, Takahashi, Yoshimori, Uchikawa, Yoshihiro, Shimazu, Ayako, Yajima, Eizo, Takashima, Takaaki, Aoba, Kiyoshi, Konishi
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Journal Title
FEMS Immunol. Med. Microbiol (in press)
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] Contribution of sialic acid-binding adhesin to pathogenesis of experimental endocarditis caused by Streptococcus gordonii DL12006
Author(s)
Yukihiro, Takahashi, Eizo, Takashima, Kisaki, Shimazu, Hisao, Yagishita, Takaaki, Aoba, Kiyoshi, Konishi
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Journal Title
Infect. Immun 74
Pages: 740-743
Description
「研究成果報告書概要(欧文)」より
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[Presentation] Identification of the erythrocyte receptor for sialic acid-binding adhesin of Streptococcus gordonii DL12007
Author(s)
Ayako, Yajima, Yumiko, Urano, Kisaki, Shimazu, Eizo, Takashima, Yukihiro, Takahashi, Kiyoshi, Konishi
Organizer
The 49th Annual Meeting of Japanese Association for Oral Biology
Place of Presentation
Sapporo
Year and Date
2007-08-31
Description
「研究成果報告書概要(欧文)」より
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[Presentation] Pathogenesis of sialic acid-binding adhesin to the experimental endocarditis caused by Streptococcus gordonii DL12006
Author(s)
Kisaki, Shimazu, Yukihiro, Takahashi, Eizo, Takashima, Ayako, Yajima, Kiyomori, Uchikawa, Takaaki, Aoba, Kiyoshi, Konishi
Organizer
The 44th Annual Meeting of The Japanese Society of Pediatric Dentistry
Place of Presentation
Matsumoto
Year and Date
2006-05-26
Description
「研究成果報告書概要(欧文)」より
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[Presentation] adhesins of oral streptococci2006
Author(s)
Yukihiro, Takahashi, Kiyoshi, Konishi
Organizer
The 79th Annual Meeting of Japanese Society for Bacteriology
Place of Presentation
Kanazawa
Year and Date
2006-03-30
Description
「研究成果報告書概要(欧文)」より
