2007 Fiscal Year Final Research Report Summary
Study on the novel bone resorbing factor on alveolar bone destruction.
| Project/Area Number |
17591932
|
|---|
| Research Category |
Grant-in-Aid for Scientific Research (C)
|
| Allocation Type | Single-year Grants |
|---|
| Section | 一般 |
|---|
| Research Field |
Morphological basic dentistry
|
|---|
| Research Institution | National Institute for Longevity Sciences,NCGG |
|---|
Principal Investigator |
NIIDA Shumpei National Institute for Longevity Sciences,NCGG, Research Institution, National Center for Geriatrics and Gerontology, 室長 (10137630)
|
|---|
| Project Period (FY) |
2005 – 2007
|
| Keywords | γ-GTP / osteoclast / bone resorption / alveolar bone / periodontitis |
|---|
| Research Abstract |
γ-Glutamyl transpeptidase(GGT) is a newly identified bone-resoibing factor which stimulates osteoclast formation. In this project, we revealed the involvements of GGT in the osteoclastic bone resorption as following :
1. GGT expression has been detected in CD45- and CD20-positive lymphocytes and plasma cells in the inflammation sites.
2. Application of GGT into rat gingival sulcus caused acute osteoclastic alveolar bone destruction.
3. We generated the monoclonal Ab against GGT, and injected them into the arthritis mice. GGT-mAb significantly diminished the number of osteoclasts and the seventy of joint destruction in arithritis mice.
4. Several bacteria(18 strains) implicated in periodontitis produced GGT.
5. We generated recombinant H. Pylori- and T. denticola GGT. Pylori GGT possessed osteoclastogenic activity in bone marrow cultures.
6. However, T. denticola GGT did not induce osteoclast formation.
These results indicate that endogenous GGT(produced by lymphocytes) acts as an enhancer for pathological osteoclast formation. GGT antagonists might be novel therapeutic agents for attenuating the bone erosion such as arthritis and periodontitis. In addition, the result that bactenal GGT stimulate osteoclastogenesis suggests that GGT generated in bacteria is involved in perodontitis-induced osteolysis. The structure of T. denticola GGT was different from that of human and Pylori's ; small molecule and short A. A. length. Since, however, GGT structures of other bacterial species are unknown, further studies are required to understand the pathology of the periodontitis.
|
-
-
-
[Journal Article] Treatment with anti-γ- glutamyl transpeptidase antibody attenuates the osteolysis in the collagen-induced arthritis mice.2007
Author(s)
Ishizuka Y, Moriwaki S, Kawahara-H M, Uemura Y, Serizawa S, Miyauchi M, Shibata S, Kanaya T, Takata T, Taniguchi N, Niida S
-
Journal Title
J Bone Miner Res 22
Pages: 1933-1942
Description
「研究成果報告書概要(欧文)」より
-
[Journal Article] Overexpression of γ-glutamyl transferase in transgenic mice accelerates bone resorption and causes osteopenia.2007
Author(s)
Hiramatsu K, Asaba Y, Takeshita S, Nimura Y, Tatsumi S, Katagiri N, Niida S, Nakajima T, Tanaka S, Ito M, Karsenty G, Ikeda K
-
Journal Title
Endocrinology 148(6)
Pages: 2708-2715
Description
「研究成果報告書概要(欧文)」より
-
-
-
-
-
-
-
-
-
-
-
-
