Molecular analysis of ANKH toward gene therapy for TMD

2006 Fiscal Year Final Research Report Summary

• Project/Area Number: 17592073
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Surgical dentistry
• Research Institution: Kyoto University
• Principal Investigator: SUGAI Manabu Kyoto University, Graduate School of Medicine, Lecturer (90303891)
• Co-Investigator(Kenkyū-buntansha): SHIMIZU Akira Kyoto University, Graduate School of Medicine, Professor (00162694) ; KOSUGI Shinzi Kyoto University, Graduate School of Medicine, Professor (50252432) ; TAKAHASHI Katsu Kyoto University, Graduate School of Medicine, Lecturer (90314202)
• Project Period (FY): 2005 – 2006
• Keywords: tooth regenaration / USAG-1 / diphyodont / polymorphism

Research Abstract

(A) Analysis of ank(progressive ankylosis) mouse
The TMJ(temporomandibular join)t of ank mouse was observed in one, two, three, four, five and six months. Mild synovitis was shown in TMJ, while severe ankylosis in other joints. Arthritis in TMJ was demonstrated in ank mouse.
(B) Polymorphic analysis of ANKH gene in TMD(temporomandibular disorder) patients
Human gene analysis in inTMD patients was approved by the medical ethics committee of Kyoto University on December 9, 2001 (#G86). As to ANK-OR and ANK-TR, which were recently reported (Florence WL, 2003) case-control study was done in about 80 cases. These involved clicking (16 cases), intermittent lock(13 cases) and closed lock (36 cases).

Research Products

• [Journal Article] Enhanced BMP signaling results in supernumerary tooth formation in USAG-1 dificient mouse (2008)
  • Author(s): Akiko Murashima-Suginami, Katsu Takahashi, Tomoko Sakata, Hiroko Tsukamoto, Manabu Sugai, Motoko Yanagita, Akira Shimizu, Takeshi Sakurai, Harold C.Slavkin and kazuhisa Bessho
  • Journal Title: Biochem Biophys Res Commun (In press)
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] Rudiment incisors survive and erupt as supernumerary teeth as a result of USAG-1 abrogation (2007)
  • Author(s): Akiko Murashima-Suginami, Katsu Takahashi, Tomohiro Kawabata, Tomoko Sakata, Hiroko Tsukamoto, Manabu Sugai, Motoko Yanagita, Akira Shimizu, Takeshi Sakurai, Harold C.Slavkin and Kazuhisa, Bessho
  • Journal Title: Biochem Biophys Res Commun 359 Pages: 549-555
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] Rudiment incisors survive and erupt as supernumerary teeth as a result of USAG-1 abrogation. (2007)
  • Author(s): Akiko Murashima-Suginami, Katsu Takahashi, Tomohiro Kawabata, Tomoko Sakata, Hiroko Tsukamoto, Manabu Sugai, Motoko Yanagita, Akira Shimizu, Takeshi Sakurai, Harold C. Slavkin, Kazuhisa Bessho.
  • Journal Title: Biochem Biophys Res Commun 359 Pages: 549-555
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Urerine sensitization-associated gene-1 (USAG-1),a nevel BMP antagonist expressed in the kidney ,accelerates tubular injury (2006)
  • Author(s): Yanagita M., Okuda, T., Endo, S., Tanaka, M., Takahashi, K., sugiyama, F., Kunita, S., Takahashi, S., Fukatsu, A., yanagisawa M., Kita, T.and Sakurai T.
  • Journal Title: J Clin Invset. 116 Pages: 70-79
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] Uterine sensitization-associated gene-1(USAG-1), a novel BMP antagonist expressed in the kidney, accelerates tubular injury. (2006)
  • Author(s): Yanagita M., Okuda, T., Endo, S., Tanaka, M., Takahashi, K., Sugiyama, F., Kunita, S., Takahashi, S., Fukatsu, A., Yanagisawa M., Kita, T., Sakurai T.
  • Journal Title: J Clin Invset. 116 Pages: 70-79
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Germline mutations of the PTCH gene in Japanese patients with nevoid basal cell carcinoma syndrome (2005)
  • Author(s): Tanioka, M., Takahashi, K., Kosugi, S., Kawabata, T., Nishigori, C., Miyachi, Y., and Iizuka, T.
  • Journal Title: Arch.Dermatol.Res 296 Pages: 303-308
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] Polymorphisms in PTCH1 affect the risk of Amelblastoma (2005)
  • Author(s): Kawabata, T., Takahashi, K., Sugai, M., Murashima-Suginami, A., Ando, S., Shumizu, A., Kosugi, S., Sato, T., Nishida, M., Murakami, K.and Iizuka, T.
  • Journal Title: J.Dent.Res 84 Pages: 812-816
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] Polymorphisms in PTCH1 affect the risk of Ameloblastoma. (2005)
  • Author(s): Kawabata, T., Takahashi, K., Sugai, M., Murashima-Suginami, A., Ando, S., Shumizu, A., Kosugi, S., Sato, T., Nishida, M., Murakami, K., Iizuka, T.
  • Journal Title: J. Dent. Res. 84 Pages: 812-816
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Germline mutations of the PTCH gene in Japanese patients with nevoid basal cell carcinoma syndrome
  • Author(s): Tanioka, M., Takahashi, K, Kosugi, S., Kawabata, T., Nishigori C., Miyachi, Y., Iizuka, T.
  • Journal Title: Arch. Dermatol. Res. 296 Pages: 303-308
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Enhanced BMP signaling results in supernumerary tooth formation in USAG-1 deficient mouse.
  • Author(s): Aldko Murashima-Suginami, Katsu Takahashi, Tomok Sakata, Hiroko Tsukamoto, Manabu Sugai, Motok Yanagita, Akira Shimizu, Takeshi Sakurai, Harold C. Slavkin, Kazuhisa Bessho.
  • Journal Title: Biochem Biophys Res Commun (in press)
  • Description: 「研究成果報告書概要(欧文)」より