The effects of anesthetics on aquaporin-5

2006 Fiscal Year Final Research Report Summary

• Project/Area Number: 17592087
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Surgical dentistry
• Research Institution: The University of Tokushima
• Principal Investigator: NAKAJO Nobuyoshi The University of Tokushima, Institute of Health Bioscience, Professor, 大学院ヘルスバイオサイエンス研究部, 教授 (80069046)
• Co-Investigator(Kenkyū-buntansha): TOMIOKA Shigemasa The University of Tokushima, Institute of Health Bioscience, Associate professor, 大学院ヘルスバイオサイエンス研究部, 助教授 (70188770) ; ISHIKAWA Yasuko The University of Tokushima, Institute of Health Bioscience, Associate professor, 大学院ヘルスバイオサイエンス研究部, 助教授 (40144985)
• Project Period (FY): 2005 – 2006
• Keywords: Aquaporin / Anesthetics / Salivation

Research Abstract

We examined the effects of anesthetic agent for the function of aquaporin-5 (AQP5) by Xenopus expression system. Oocytes from Xenopus Laevis microinjected with in vitro-transcripted AQP5 mRNA exhibited increased these volume; this was reversibly inhibited by mercuric chloride, a known inhibitor of water channels. The rate of volume change due to osmotic effects was imaged on the camera connected to an area analyzer. Serial images were stored at 1-sec intervals in a computer. We compared actions of different anesthetic drugs, such as pentobarbital (50-1000 uM), propofol(1-200 uM), ketamine (1-100 uM), midazolam (1-200 uM), sevoflurane (1 mM) and isoflurane (1 mM), lidocaine (1 uM), benzocaine (1 uM) and QX-314 (1 uM). The ratio of volume change was inhibited by pentobarbital and propofol while ketamine and midazolam had no effect. On the other hand, sevoflurane and isoflurane increased the ratio of volume change by AQP5. Lidocaine, benzocaine (permanently uncharged) and QX-314 (permanently charged) had no effects for the ratio of volume change. These results suggest that some anesthetics may indeed affect the function of AQP5.