2006 Fiscal Year Final Research Report Summary
Development of novel therapy for TMJ arthritis by Gene transfer using with sonoporation combined with microbubble-based ultrasound contrast agent
| Project/Area Number |
17592097
|
|---|
| Research Category |
Grant-in-Aid for Scientific Research (C)
|
| Allocation Type | Single-year Grants |
|---|
| Section | 一般 |
|---|
| Research Field |
Surgical dentistry
|
|---|
| Research Institution | Kyushu Dental College |
|---|
Principal Investigator |
TOMINAGA Kazuhiro Kyushu Dental College, Dept. of Oral and Maxillofacial Surgery, Associate Professor (40188793)
|
|---|
| Co-Investigator(Kenkyū-buntansha) |
NISHIBARA Tatsuji Kyushu Dental College, Dept of Health Promotion, Professor (80192251)
|
|---|
| Project Period (FY) |
2005 – 2006
|
| Keywords | temporomandibular disorder / ultrasonographic gene induction / interleukin-1 / IL-1 receptor antagonist / svnovial cells / microbubble / PGE2 / anti-inflammatory effect |
|---|
| Research Abstract |
Administration of exogenous IL-1 receptor antagonist (IL-1ra) is effective in reducing the inflammatory reactions mediated by IL-1. In the present study, we transfected with a plasmid containing IL-1ra gene into synovial cells by the sonoporation method, and investigated the effect of IL-1ra gene transfection on inflammatory reactions induced by lipopolysaccharide (LPS) in vitro. IL-1ra- transfected synovial cells produced the extracellular IL-lra, and its level increased remarkably when the cells were cultured with LPS for 48 h. Interestingly, treatment of LPS enhanced the release of IL-1β in the culture supernatant of IL-1ra-transfected cells, however, a significant decrease of PGE2 release was observed when the cells were cultured with LPS for 60 h. These results indicate that IL-1ra could play a valuable regulatory role in inflammatory reactions of synovial cells, suggesting that the administration of IL-1ra gene with sonoporation is a useful method for the control of inflammation in arthritis.
|
-
-
-
-
-
[Journal Article] Fukuda, j.and Ohba, T. : Relationship between cortical bone formation on mandibular condyles and alternation of the magnetic resonance signals characteristic of growth.2007
Author(s)
Morimoto, Y., Konoo, T., Tominaga, K., Tanaka, T., Yamaguchi, K., Fukuda, j. Ohba, T.
-
Journal Title
Am J Orthod Dentofacial Orthop 131(4)
Pages: 473-480
Description
「研究成果報告書概要(欧文)」より
-
[Journal Article] Local delivery system of cytotoxic agents by focued sonoporation.2007
Author(s)
Iwanaga, K., Tominaga, K., Yamamoto, K., Habu, M., Maeda, H., Akifusa, S., Tsujusawa, T., Okinaga, T., Fukuda, J., Nishihara, ' T.
-
Journal Title
Cancer Gene Therapy 14
Pages: 351-363
Description
「研究成果報告書概要(欧文)」より
-
[Journal Article] Intraoperative surgical clearance confirmation of tongue carcinomas using ultrasonography.2007
Author(s)
Tominaga, K., Yamamoto, K., Khanal, A., Morimoto, Y., Tanaka, T., Kodama, M., Fukuda, J
-
Journal Title
Dentomaxillofac Radiol 36
Pages: 409-411
Description
「研究成果報告書概要(欧文)」より
-
[Journal Article] Changes in temporomandibular disc position during growth in young Japanese.2007
Author(s)
Tominaga, K., Konoo, T., Morimoto, Y., Tanaka, T., Habu, M. Fukuda, J.
-
Journal Title
Dentomaxillofac Radiol 36
Pages: 397-401
Description
「研究成果報告書概要(欧文)」より
-
-
-
[Journal Article] A simple method for bone positioning of mandibular segments.2006
Author(s)
Tominaga, K., Yoshioka, I., Khanal, A., Furuta, N., Habu, M. Fukuda, J
-
Journal Title
mt J Oral Maxillofac Surg 35
Pages: 856-860
Description
「研究成果報告書概要(欧文)」より
-
[Journal Article] Biomechanical evaluation of different types of rigid internal fixation techniques for subcondylar fractures.2006
Author(s)
Tominaga, K., Habu, M., Khanal, A., Mimori, Y., Yoshioka, I. Fukuda, J.
-
Journal Title
J Oral Maxillofac Surg 41
Pages: 1510-1516
Description
「研究成果報告書概要(欧文)」より
