2007 Fiscal Year Final Research Report Summary
Biofilm eliminative strategy for Periodontal therapy
Project/Area Number |
17592165
|
Research Category |
Grant-in-Aid for Scientific Research (C)
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Allocation Type | Single-year Grants |
Section | 一般 |
Research Field |
Periodontal dentistry
|
Research Institution | Tokyo Dental College |
Principal Investigator |
ITO Akiyo Tokyo Dental College, Departments of Periodontology, Assistant Professor (00307381)
|
Co-Investigator(Kenkyū-buntansha) |
ITO Tachi Tokyo Dental College, Departments of Oral and Maxillofacial Implantology, Assistant Professor (80312015)
KATO Tetsuo Tokyo Dental College, Departments of Microbiology, Associate Professor (00159253)
ISHIHARA Kazuyuki Tokyo Dental College, Departments of Microbiology, Associate Professor (00212910)
YAMADA Satoru Tokyo Dental College, Departments of Periodontology, Professor (20103351)
OKUDA Katsuji Tokyo Dental College, Departments of Microbiology, Professor (40085741)
|
Project Period (FY) |
2005 – 2007
|
Keywords | Biofilm / Glycocalyx / Periodontal associated bacteria / Quorum sensing / acyl-homoserine-lactones(AHL) / Actinomyces |
Research Abstract |
Recently, dental plaque has characterized as biofilm, and its formation are associated with intercellular communication ; quorum sensing (QS). Therefore qs, the density dependent regulation of gene expression for facilitating environmental adaptation, is widespread among gram negative and gram positive bacteria. QS bacteria synthesize and secrete extracellular signaling molecules called autoinducer., which accumulate in the enviroment as the population increases.Although previous researchers were unable to obtain any evidence to suggest that the Gram-negative anaerobe Porphyromonas gingivalis employs AHL-mediated QS(AI-1), they have demonstrated that this periodontal associated bacteria produces a diffusible signal molecule capable of activating the Vibrio harveyi AI-2 biosensor. In the present study, we investigated the influence of synthetic N-acyl homoserine lactones (N-acyl HSLs), signaling compounds for quorum sensing, on the growth and production of proteins in periodontopathic bacteria Porphyromonas gingivalis. N-butyryl HSL, N-hexanoyl HSL, N-heptanoyl HSL, N-octanoyl HSL, and N-tetradecanoyl (myristoyl) HSL were used as synthetic N-acyl HSLs (Sigma-Aldrich). N-tetradecanoyl HSL inhibited the growth of all P. gingivalis strains used in this study in a dose-dependent manner. When we compared the SDS-PAGE profiles of sonicated samples of P gingivalis bacterial cells cultured with/without N-tetradecanoyl HSL, we found that protein production was changing. The growth of P gingivalis was inhibited by myristoyl coenzyme A (CoA) but did not by lauroyl CoA. These results indicated that P gingivalis responded to auto-produced N-tetradecanoyl HSL-like molecule and slowed their growth. Actinomyces viscousus , which is early colonizer in oral biofilm was tested in this study. Actinomyces strains were cultured by using flowcell system. Microscopic observation exhibited that some Actinomycesstrains formed microcolonies.
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[Journal Article] Relationship Between Antimicrobial Protein Levels in Whole Saliva and Periodontitis2008
Author(s)
Ito, T., Komiya-Ito, A., Arataki, T., Furuya, Y., Yajima, Y, Yamada, S., Okuda, K., Kato, T.
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Journal Title
J.Periodontol. 79(2)
Pages: 316-322
Description
「研究成果報告書概要(和文)」より
Peer Reviewed
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[Journal Article] Relationship Between Antimicrobial Protein Levels in Whole Saliva and Periodontitis2008
Author(s)
Ito, T., Komiya-Ito, A., Arataki, T., Furuya, Y., Yajima, Y., Yamada, S., Okuda, K., Kato, T
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Journal Title
J. Periodontol 79(2)
Pages: 316-322
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] N-Tetradecanoyl H A.omoserine Lactone,Signaling Compound for Quorum sensing,Inhibits Porphyromonas gingivalis Growth2006
Author(s)
Komiya-Ito, A., Ito, T., Yamanaka, A., Okuda, K., Yamada, S., and Kato, T.
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Journal Title
Res.J.Microbiol. 1(4)
Pages: 353-359
Description
「研究成果報告書概要(和文)」より
Peer Reviewed
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[Journal Article] N-Tetradecanoyl H A.omoserine Lactone, Signaling Compound for Quorum sensing, Inhibits Porphyromonas gingivalis Growth.2006
Author(s)
Komiya-Ito, A., Ito, T., Yamanaka, A., Okuda, K., Yamada, S., Kato, T
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Journal Title
Res. J. Microbiol 1(4)
Pages: 353-359
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] Murine monoclonal antibody which can distinguish cystatins SA1 and SA22005
Author(s)
Ito, T., Ito, A., Okuda, K., Minaguchi, K., Saitoh, E., Yamada, S., Kato, T.
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Journal Title
Mol.Immuno. 42(10)
Pages: 1259-1263
Description
「研究成果報告書概要(和文)」より
Peer Reviewed
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[Journal Article] Murine monoclonal antibody which can distinguish cystatins SAl and SA22005
Author(s)
Ito, T., Ito, A., Okuda, K., Minaguchi, K., Saitoh, E. Yamada, S., Kato, T
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Journal Title
Mol. Immuno 42(10)
Pages: 1259-1263
Description
「研究成果報告書概要(欧文)」より
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[Presentation] 歯周炎およびインプラント周囲炎患者における唾液中抗菌タンパク質と歯周病関連細菌との関連性2007
Author(s)
伊藤 太一, 伊藤 明代, 北川 博美, 法月 良江, 伊藤 寛史, 吉田 有智, 小田 貴士, 猿田 浩規, 安田 雅章, 鈴木 憲久, 古谷 義隆, 荒瀧 友彦, 奥田 克爾, 加藤 哲男, 武田 孝之, 椎貝 達夫, 飯島 俊一, 添島 義和, 矢島 安朝
Organizer
第37回日本口腔インプラント学会学術大会
Place of Presentation
熊本市
Year and Date
2007-09-15
Description
「研究成果報告書概要(和文)」より
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[Presentation] クランベリーの口腔内バイオフィルム形成阻害効果2007
Author(s)
山中 あゆみ, 君塚 隆太, 河内 智子, 葛西 浩一, 宝田 恭子, 伊藤 明代, 山田 了, 齋藤 百合子, 中川 寛一, 齋藤 貴之, 櫻井 薫, 石原 和幸, 加藤 哲男, 奥田 克爾
Organizer
歯科医学を中心とした総合的な研究を推進する集い
Place of Presentation
東京
Year and Date
2007-01-13
Description
「研究成果報告書概要(和文)」より
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[Presentation] 東京歯科大学千葉病院総合診療科における臨床研修歯科医の診療状況について2006
Author(s)
高橋 俊之, 角田 正健, 山倉 大紀, 杉山 利子, 宮下 有恒, 安達 亮, 森永 一喜, 伊藤 明代, 春山 親弘, 武田 友孝, 中島 一憲
Organizer
東京歯科大学学会総会
Place of Presentation
千葉市,東京歯科大学
Year and Date
2006-11-04
Description
「研究成果報告書概要(和文)」より
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[Presentation] 唾液中抗菌タンパク質と歯周炎,インプラント周囲炎の関連性2005
Author(s)
伊藤 太一, 伊藤 明代, 荒瀧 友彦, 古谷 義隆, 渋川 義宏, 水口 清, 山田 了, 奥田 克爾, 矢島 安朝, 加藤 哲男
Organizer
日本口腔インプラント学会総会、学術大会
Place of Presentation
弘前市
Year and Date
20050916-18
Description
「研究成果報告書概要(和文)」より
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