Biofilm eliminative strategy for Periodontal therapy

2007 Fiscal Year Final Research Report Summary

• Project/Area Number: 17592165
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Periodontal dentistry
• Research Institution: Tokyo Dental College
• Principal Investigator: ITO Akiyo Tokyo Dental College, Departments of Periodontology, Assistant Professor (00307381)
• Co-Investigator(Kenkyū-buntansha): ITO Tachi Tokyo Dental College, Departments of Oral and Maxillofacial Implantology, Assistant Professor (80312015) ; KATO Tetsuo Tokyo Dental College, Departments of Microbiology, Associate Professor (00159253) ; ISHIHARA Kazuyuki Tokyo Dental College, Departments of Microbiology, Associate Professor (00212910) ; YAMADA Satoru Tokyo Dental College, Departments of Periodontology, Professor (20103351) ; OKUDA Katsuji Tokyo Dental College, Departments of Microbiology, Professor (40085741)
• Project Period (FY): 2005 – 2007
• Keywords: Biofilm / Glycocalyx / Periodontal associated bacteria / Quorum sensing / acyl-homoserine-lactones(AHL) / Actinomyces

Research Abstract

Recently, dental plaque has characterized as biofilm, and its formation are associated with intercellular communication ; quorum sensing (QS). Therefore qs, the density dependent regulation of gene expression for facilitating environmental adaptation, is widespread among gram negative and gram positive bacteria. QS bacteria synthesize and secrete extracellular signaling molecules called autoinducer., which accumulate in the enviroment as the population increases.Although previous researchers were unable to obtain any evidence to suggest that the Gram-negative anaerobe Porphyromonas gingivalis employs AHL-mediated QS(AI-1), they have demonstrated that this periodontal associated bacteria produces a diffusible signal molecule capable of activating the Vibrio harveyi AI-2 biosensor.
In the present study, we investigated the influence of synthetic N-acyl homoserine lactones (N-acyl HSLs), signaling compounds for quorum sensing, on the growth and production of proteins in periodontopathic bacteria Porphyromonas gingivalis. N-butyryl HSL, N-hexanoyl HSL, N-heptanoyl HSL, N-octanoyl HSL, and N-tetradecanoyl (myristoyl) HSL were used as synthetic N-acyl HSLs (Sigma-Aldrich). N-tetradecanoyl HSL inhibited the growth of all P. gingivalis strains used in this study in a dose-dependent manner. When we compared the SDS-PAGE profiles of sonicated samples of P gingivalis bacterial cells cultured with/without N-tetradecanoyl HSL, we found that protein production was changing. The growth of P gingivalis was inhibited by myristoyl coenzyme A (CoA) but did not by lauroyl CoA. These results indicated that P gingivalis responded to auto-produced N-tetradecanoyl HSL-like molecule and slowed their growth.
Actinomyces viscousus , which is early colonizer in oral biofilm was tested in this study. Actinomyces strains were cultured by using flowcell system. Microscopic observation exhibited that some Actinomycesstrains formed microcolonies.

Research Products

• [Journal Article] Relationship Between Antimicrobial Protein Levels in Whole Saliva and Periodontitis (2008)
  • Author(s): Ito, T., Komiya-Ito, A., Arataki, T., Furuya, Y., Yajima, Y, Yamada, S., Okuda, K., Kato, T.
  • Journal Title: J.Periodontol. 79(2) Pages: 316-322
  • Description: 「研究成果報告書概要(和文)」より
  • Peer Reviewed
• [Journal Article] Relationship Between Antimicrobial Protein Levels in Whole Saliva and Periodontitis (2008)
  • Author(s): Ito, T., Komiya-Ito, A., Arataki, T., Furuya, Y., Yajima, Y., Yamada, S., Okuda, K., Kato, T
  • Journal Title: J. Periodontol 79(2) Pages: 316-322
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] N-Tetradecanoyl H A.omoserine Lactone,Signaling Compound for Quorum sensing,Inhibits Porphyromonas gingivalis Growth (2006)
  • Author(s): Komiya-Ito, A., Ito, T., Yamanaka, A., Okuda, K., Yamada, S., and Kato, T.
  • Journal Title: Res.J.Microbiol. 1(4) Pages: 353-359
  • Description: 「研究成果報告書概要(和文)」より
  • Peer Reviewed
• [Journal Article] N-Tetradecanoyl H A.omoserine Lactone, Signaling Compound for Quorum sensing, Inhibits Porphyromonas gingivalis Growth. (2006)
  • Author(s): Komiya-Ito, A., Ito, T., Yamanaka, A., Okuda, K., Yamada, S., Kato, T
  • Journal Title: Res. J. Microbiol 1(4) Pages: 353-359
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Murine monoclonal antibody which can distinguish cystatins SA1 and SA2 (2005)
  • Author(s): Ito, T., Ito, A., Okuda, K., Minaguchi, K., Saitoh, E., Yamada, S., Kato, T.
  • Journal Title: Mol.Immuno. 42(10) Pages: 1259-1263
  • Description: 「研究成果報告書概要(和文)」より
  • Peer Reviewed
• [Journal Article] Murine monoclonal antibody which can distinguish cystatins SAl and SA2 (2005)
  • Author(s): Ito, T., Ito, A., Okuda, K., Minaguchi, K., Saitoh, E. Yamada, S., Kato, T
  • Journal Title: Mol. Immuno 42(10) Pages: 1259-1263
  • Description: 「研究成果報告書概要(欧文)」より
• [Presentation] 歯周炎およびインプラント周囲炎患者における唾液中抗菌タンパク質と歯周病関連細菌との関連性 (2007)
  • Author(s): 伊藤 太一, 伊藤 明代, 北川 博美, 法月 良江, 伊藤 寛史, 吉田 有智, 小田 貴士, 猿田 浩規, 安田 雅章, 鈴木 憲久, 古谷 義隆, 荒瀧 友彦, 奥田 克爾, 加藤 哲男, 武田 孝之, 椎貝 達夫, 飯島 俊一, 添島 義和, 矢島 安朝
  • Organizer: 第37回日本口腔インプラント学会学術大会
  • Place of Presentation: 熊本市
  • Year and Date: 2007-09-15
  • Description: 「研究成果報告書概要(和文)」より
• [Presentation] クランベリーの口腔内バイオフィルム形成阻害効果 (2007)
  • Author(s): 山中 あゆみ, 君塚 隆太, 河内 智子, 葛西 浩一, 宝田 恭子, 伊藤 明代, 山田 了, 齋藤 百合子, 中川 寛一, 齋藤 貴之, 櫻井 薫, 石原 和幸, 加藤 哲男, 奥田 克爾
  • Organizer: 歯科医学を中心とした総合的な研究を推進する集い
  • Place of Presentation: 東京
  • Year and Date: 2007-01-13
  • Description: 「研究成果報告書概要(和文)」より
• [Presentation] 東京歯科大学千葉病院口腔インプラント科来院患者における歯周病罹患状況について (2006)
  • Author(s): 伊藤 太一, 伊藤 明代, 荒瀧 友彦, 古谷 義隆, 山田 了, 武田 孝之, 飯島 俊一, 椎貝 達夫, 添島 義和, 矢島 安朝
  • Organizer: 第36回日本口腔インプラント学会学術大会
  • Place of Presentation: 新潟市
  • Year and Date: 20060915-17
  • Description: 「研究成果報告書概要(和文)」より
• [Presentation] 東京歯科大学千葉病院総合診療科における臨床研修歯科医の診療状況について (2006)
  • Author(s): 高橋 俊之, 角田 正健, 山倉 大紀, 杉山 利子, 宮下 有恒, 安達 亮, 森永 一喜, 伊藤 明代, 春山 親弘, 武田 友孝, 中島 一憲
  • Organizer: 東京歯科大学学会総会
  • Place of Presentation: 千葉市,東京歯科大学
  • Year and Date: 2006-11-04
  • Description: 「研究成果報告書概要(和文)」より
• [Presentation] 唾液中抗菌タンパク質と歯周炎,インプラント周囲炎の関連性 (2005)
  • Author(s): 伊藤 太一, 伊藤 明代, 荒瀧 友彦, 古谷 義隆, 渋川 義宏, 水口 清, 山田 了, 奥田 克爾, 矢島 安朝, 加藤 哲男
  • Organizer: 日本口腔インプラント学会総会、学術大会
  • Place of Presentation: 弘前市
  • Year and Date: 20050916-18
  • Description: 「研究成果報告書概要(和文)」より
• [Presentation] ニコチンがサイトカインネットワークと歯周病原細菌バイオフィルムにおよぼす影響 (2005)
  • Author(s): 牧野 麻子, 加藤 哲男, 高橋 尚子, 伊藤 明代, 林 智子, 奥田 克爾, 山田 了
  • Organizer: 第280回東京歯科大学学会総会
  • Place of Presentation: 千葉市,東京歯科大学
  • Year and Date: 2005-10-15
  • Description: 「研究成果報告書概要(和文)」より