2018 Fiscal Year Annual Research Report
Field-assisted assembly of the DNA networks for electronics and biologydcal applications
| Project/Area Number |
17F17065
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| Research Institution | Osaka University |
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Principal Investigator |
松本 卓也 大阪大学, 理学研究科, 教授 (50229556)
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| Co-Investigator(Kenkyū-buntansha) |
LIVSHITS GIDEON 大阪大学, 理学(系)研究科(研究院), 外国人特別研究員
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| Project Period (FY) |
2017-04-26 – 2019-03-31
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| Keywords | nanopipette / DNA / patterning / surface modification |
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| Outline of Annual Research Achievements |
In the final year of the JSPS fellowship (2018-2019), we established new tools and methodologies to address technical difficulties encountered during our research. Most significantly, we succeeded in optimizing buffer conditions for the deposition of ligated λ-DNA (~100 µm in length) on APTES-coated glass slides using the nanopipette with a counter electrode. Imaging of the patterned lines was done in a fluorescence microscope.
We successfully implemented new strategies to overcome significant resistant to flow of water and liquid metals as channel cross section decreases in diameter, from ~500 um to ~100 nm:
1. A new pipette holder was constructed, enabling simultaneous application of high pressure (up to ~200 Atm), heating (up to ~500 C) and high voltage (up to 10 kV) to pipette. Using the new holder, we were able to extrude GaIn alloy through the nanopipette channel.
2. Protocol for coating of the inside of glass capillaries with gold using electroless plating was established, with successful reproducible results. Gold coated nanopipettes will be used for various applications.
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| Research Progress Status |
平成30年度が最終年度であるため、記入しない。
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| Strategy for Future Research Activity |
平成30年度が最終年度であるため、記入しない。
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