2018 Fiscal Year Annual Research Report
ＮＩＲＳとアクアフォトミクス法を用いた唾液と乳房組織による乳牛の乳房炎の診断法
| Project/Area Number |
17F17406
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| Research Institution | Kobe University |
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Principal Investigator |
ツエンコヴア ルミアナ 神戸大学, 農学研究科, 教授 (30294200)
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| Co-Investigator(Kenkyū-buntansha) |
MUNCAN JELENA 神戸大学, 農学研究科, 外国人特別研究員
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| Project Period (FY) |
2017-10-13 – 2020-03-31
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| Keywords | aquaphotomics / NIR spectroscopy / tissue / body fluids / disease diagnosis / monitoring |
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| Outline of Annual Research Achievements |
The 4 main activities were planned and carried out with the following results:
1) Experimental work: in collaboration with Awajishima Agricultural Center collection of milk and tissue spectra performed as planned. Resulting in a database of more than 40,000 spectra.
2) Preparation for analysis: Developed databases for each animal in order to establish individual biomonitoring. In addition to spectral information, data on the physiological state, living conditions and composition of milk, rumen juice and blood (standard components, fatty acids, glucose etc.) for the period of two weeks were also obtained.
3) Exploratory data analysis: performed as planned and resulted in identification of strong influence of individuality of each animal. Correction tools were applied and general models for detection of certain components in milk were developed which provided information about the general state of the animals in the followed period and provided feedback which can be used for correction in animal diet.
4) Training and dissemination of results: Continued aquaphotomics training and analysis of existing laboratory data performed as planned. Analysis of other biological systems such as plants, as well as less complex systems such as aqueous solutions resulted in improvements in understanding of water roles in biological systems and development of chemometrics tools for better extraction of relevant data from the spectra. Dissemination performed by publishing two papers, one protocol, two oral presentations and two poster presentations at international conferences.
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| Current Status of Research Progress |
Current Status of Research Progress
2: Research has progressed on the whole more than it was originally planned.
Reason
All the experiments and preparation of data for analysis went as planned. The collected data comprise really huge source of information. Analysis is progressing as planned, with exploratory analysis finished and progressing to discrimination analysis and regression analysis with goals of detection of disease and physiological states. In addition, analysis was widen to include regression analysis on milk components using aquaphotomics, to include measurements of fatty acids content, which was not previously performed and was in the previous period detected as possible and valuable source of feedback for management of dairy animals nutrition and breeding. In parallel, review of aquaphotomics literature as well as training in data analysis based on already existing laboratory data was performed and resulted in two publications. The Biomeasurement Technology Laboratory provided extensive database of spectral data coming from different aqueous and biological systems, which provided deeper understanding of the water functionality in different conditions of the systems, as well as development of novel strategies for improved data analysis.
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| Strategy for Future Research Activity |
The future plans include cross-analysis of near infrared spectral databases for each animal and cross-comparison with all the other animals in order to detect daily events characteristic for each animal. Relating “events” detected as characteristic for the animal with the existing information on animal status allows detection of the patterns in spectral data which signal an occurrence of disease or changes in physiological status. This approach in addition to exploratory, discriminating and regression analysis would allow faster tracking of events such as disease development. Plan also includes continued aquaphotomics training and analysis of existing laboratory data on other biological systems and working on publications and dissemination of achieved results via scientific journals and presentations at the international conferences. The previous period resulted in writing 4 new papers which are expected to be published in the next period. Two poster presentations, one oral presentation and a workshop are in plan for the international conference ICNIRS, which will be held in September 15-20th, in Australia. The book on application of aquaphotomics in dairy cows biomonitoring is in the process of writing and expected to be submitted for publishing in June 2019.
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[Journal Article] Protocol for aquaphotomics monitoring of water molecular structure in leaves of resurrection plants during desiccation and recovery2019
Author(s)
Muncan, J., Kuroki, S., Moyankova, D., Morita, H., Atanassova, S., Djilianov, D., Tsenkova, R.
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Journal Title
Protocol Exchange
Volume: -
Pages: -
DOI
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[Presentation] The structure of water in soft contact lenses: near infrared spectroscopy and Aquaphotomics2019
Author(s)
Muncan, J., Sakota-Rosic, J., Mileusnic, I., Matovic, V., Matija, L., Tsenkova, R.
Organizer
in Proceedings of the 18th International Conference on Near Infrared Spectroscopy
Int'l Joint Research
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