2021 Fiscal Year Final Research Report
Novel membrane proteins by combining computational design and in vitro directed eovlution
| Project/Area Number |
17H00888
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| Research Category |
Grant-in-Aid for Scientific Research (A)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Chemical biology
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| Research Institution | Tokyo Institute of Technology (2020-2021)
Osaka University (2017-2019) |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
大崎 寿久 地方独立行政法人神奈川県立産業技術総合研究所, 人工細胞膜システムグループ, サブリーダー (50533650)
藤井 聡志 地方独立行政法人神奈川県立産業技術総合研究所, 人工細胞膜システムグループ, 研究員 (60619005)
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| Project Period (FY) |
2017-04-01 – 2021-03-31
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| Keywords | 膜タンパク質 / リポソーム / 進化分子工学 |
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| Outline of Final Research Achievements |
Only a small fraction of the sequence space has been explored by nature through evolution. By rational design, it is now clear that for soluble proteins, there are many artificial proteins that possess structures and functions in the unexplored sequence space. In this study, we show that there are molecules with artificial membrane proteins that also have functions and structures in membrane proteins. Specifically, we designed an artificial membrane protein with a 2 nm pore and clarified its function using an artificial cell reactor.
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| Free Research Field |
合成生物学
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| Academic Significance and Societal Importance of the Research Achievements |
人工細胞を構築する研究は、非生命から生命をどのように創り出せるかを明らかにする方法の一つである。現在までに、細胞の機能を模倣した様々な人工細胞が構築されてきた。本研究課題では、人工細胞が人工膜タンパク質の機能解析に用いること、更には、膜タンパク質進化分子工学に使えることを示すものである。
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