Chemical Biology Studies of Ubiquitin Transfer with Synthetic Proteins

2021 Fiscal Year Annual Research Report

• Project/Area Number: 17H01211
• Research Institution: Nagoya University
• Principal Investigator: Bode Jeffrey 名古屋大学, トランスフォーマティブ生命分子研究所, 客員教授 (90727900)
• Project Period (FY): 2017-04-01 – 2022-03-31
• Keywords: タンパク質化学 / 有機合成化学 / プロテオミクス解析 / ユビキチン化 / ケミカルバイオロジー

Outline of Annual Research Achievements

We attempted to trap the unknown E3 ligases for CRY1 protein, which is involved in regulating the circadian rhythms. We identified several candidate E3 ligases responsible for modifications of CRY1 by using our synthetic E2-SUMO probes in cell lysates for photo crosslinking. We are working on the validation of our hits with our collaborator, Dr. Tsuyoshi Hirota using siRNA knock downs of the candidate genes.
We continued to synthesize new E2 conjugating enzymes. Also, we worked on the preparation of Ubiquitin and Ubiquitin-like proteins that can trap E2 conjugating enzymes for pulled down assays.

Research Progress Status

令和3年度が最終年度であるため、記入しない。

Strategy for Future Research Activity

令和3年度が最終年度であるため、記入しない。