Frontier of research on antiviral immunity in phytopathogenic filamentous fugi

2020 Fiscal Year Annual Research Report

• Project/Area Number: 17H01463
• Research Institution: Okayama University
• Principal Investigator: 鈴木 信弘 岡山大学, 資源植物科学研究所, 教授 (70206514)
• Co-Investigator(Kenkyū-buntansha): 近藤 秀樹 岡山大学, 資源植物科学研究所, 准教授 (40263628) ; 兵頭 究 岡山大学, 資源植物科学研究所, 准教授 (80757881)
• Project Period (FY): 2017-04-01 – 2021-03-31
• Keywords: RNAi / マイコイミュニティ / dsRNA / 菌類ウイルス / マイコウイルス / RNAサイレンシング / サイレンシングサプレッサー / Dicer

Outline of Annual Research Achievements

菌糸の融合不和合は固体集団レべルでのウイルス免疫(ウイルス水平伝搬の難易)に関与する。そこでこの融合不和合を規定する遺伝子とRNAi関連遺伝子の破壊株、ノックダウン株をクリ胴枯病菌で作製する。「この菌(免疫不全菌)系統は、免疫不全マウスと同じように各種ウイルスの宿主となり、しかも不和合の組み合せの受容菌側の細胞死抑制」が期待される。まず、本菌の非相同組換え欠損系統DK80を遺伝的背景として，Dicer様タンパク質遺伝子(dcl2)ならびにArgonaute様タンパク質遺伝子(agl1, agl2, agl3, agl4)の5重変異体の作成に成功した。これら菌株間で数科のRNAウイルスの蓄積量を比較した。その結果，いくつかのRNAサイレンシング感受性ウイルスに対しては，Argonauteを必要とせずDicerがだけで複製を阻害できること（防御機構）が示された。しかし、別のウイルスの複製阻害にArgonauteを必要とする。この成果はRNAiのパラダイムシフトをもたらす大きな成果である。
申請者らは、既に菌類で機能するRNAi抑制蛋白質の評価法を開発し、CHV1p29を同定した。岡山大で保有する50を超える菌類ウイルスの中から、さらに抑制蛋白質CHV4-p24を同定した(Aulia et al., Biology 2021)。p29に加えp24RNAi抑制蛋白質の作用点を細胞学的・分子生物学的アフプローチで解析する。CHV4-p24発現クリ胴枯病菌ではRNAサイレンシング感受性ウイルスMyRV2が安定的に維持され、蓄積量も増加した。一方、CHV1-p29と相互作用するする宿主タンパク質を免疫沈降／MS解析により多数同定した。現在、それらが果たすであろうCHV1複製、RNAサイレンシング抑制での役割を遺伝子破壊により解析中である。

Research Progress Status

令和2年度が最終年度であるため、記入しない。

Strategy for Future Research Activity

令和2年度が最終年度であるため、記入しない。

Research Products

• [Int'l Joint Research] Bradley I. Hillman/Rutgers University(米国)
  • Country Name: U.S.A.
  • Counterpart Institution: Bradley I. Hillman/Rutgers University
• [Int'l Joint Research] Atif Jamal/National Agricultural Research Centre(パキスタン)
  • Country Name: PAKISTAN
  • Counterpart Institution: Atif Jamal/National Agricultural Research Centre
• [Journal Article] A moderate level of hypovirulence conferred by a hypovirus in the avocado white root rot fungus, Rosellinia necatrix. (2021)
  • Author(s): Arjona-Lopez, J. M., Telengech, P., Suzuki, N., and Lopez-Herrera, C.
  • Journal Title: Fungal Biology Volume: 125 Pages: 69-76
  • DOI: 10.1016/j.funbio.2020.10.007
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Cryphonectria nitschkei chrysovirus 1 with unique molecular features and a very narrow host range. (2021)
  • Author(s): Shahi, S., Chiba, S., Kondo, H., and Suzuki, N.
  • Journal Title: Virology Volume: 554 Pages: 55-65
  • DOI: 10.1016/j.virol.2020.11.011
  • Peer Reviewed / Open Access
• [Journal Article] Biochemical characterization of the dicing activity of Dicer-like 2 in the model filamentous fungus Neurospora crassa. (2021)
  • Author(s): Tabara, M., Koiwa, H., Suzuki, N., and Fukuhara, T.
  • Journal Title: Fungal Genetics and Biology Volume: 146 Pages: 103488
  • DOI: 10.1016/j.fgb.2020.103488
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] In-tree behavior of diverse viruses harbored in the chestnut blight fungus, Cryphonectria parasitica. (2021)
  • Author(s): Suzuki, N., Aulia, A., Shahi, S., Hillman, B. I., Cornerjo, C., Rigling, D.
  • Journal Title: Journal of Virology Volume: 95 Pages: e01962-20
  • DOI: 10.1128/JVI.01962-20
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Virome analysis of aphid populations that infest the barley field: the discovery of two novel groups of nege/kita-like viruses and other novel RNA viruses. (2020)
  • Author(s): Kondo, H., Fujita, M., Hisano, H., Hyodo, K., Andika I. B., and Suzuki, N.
  • Journal Title: Front. Microbiol. Volume: 11 Pages: 509
  • DOI: 10.3389/fmicb.2020.00509
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Diverse partitiviruses from the phytopathogenic fungus, Rosellinia necatrix. (2020)
  • Author(s): Telengech, P., Hisano, S., Micheni, C. M., Hyodo, K., Arjona-Lopez, J.M., Lopez-Herrera, C., Kanematsu, S., Kondo, H., and Suzuki, N.
  • Journal Title: Frontiers in Microbiology Volume: 11 Pages: 1064
  • DOI: 10.3389/fmicb.2020.01064
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Hadaka virus 1: A capsidless 11-segmented (+)RNA virus from a phytopathogenic fungus Fusarium oxysporum. (2020)
  • Author(s): ato, Y., Shamsi, W., Jamal, A., Bhatti, M. F., Kondo, H., and Suzuki, N.
  • Journal Title: mBio Volume: 11 Pages: e0045-20
  • DOI: 10.1128/mBio.00450-20
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Dicer monitoring in a model filamentous fungus host, Cryphonectria parasitica. (2020)
  • Author(s): Aulia, A., Tabara, M., Telengech, P., Fukuhara, T., and Suzuki, N.
  • Journal Title: Current Research in Virological Science Volume: 1 Pages: 100001
  • DOI: 10.1016/j.crviro.2020.100001
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Coinfection of Rosellinia necatrix by a partitivirus and a virga-like virus is associated with hypovirulence. (2020)
  • Author(s): Arjona-Lopez, J. M., Telengech, P., Suzuki, N., and Lopez-Herrera, C.
  • Journal Title: European Journal of Plant Pathology Volume: 158 Pages: 111-119
  • DOI: 10.1007/s10658-020-02058-x
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Molecular characterization of a novel polymycovirus from Penicillium janthinellum with a focus on its genome-associated PASrp. (2020)
  • Author(s): Sato, Y., Jamal, A., Kondo, H., and Suzuki, N.
  • Journal Title: Frontiers in Microbiology Volume: 11 Pages: 592789
  • DOI: 10.3389/fmicb.2020.592789
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Establishment of Neurospora crassa as a model organism for fungal virology. (2020)
  • Author(s): Honda, S., Ana Eusebio-Cope, A., Miyashita, S., Yokoyama, A., Aulia, A., Shahi, S., Kondo, H., and Suzuki, N.
  • Journal Title: Nature Communications Volume: 11 Pages: 5627.
  • DOI: 10.1038/s41467-020-19355-y
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Structure and assembly of double-stranded RNA mycovirus (2020)
  • Author(s): Mata, C. P., Rodriguez, J. M., Suzuki, N., and Caston, J. R.
  • Journal Title: Advances in Virus Research Volume: 108 Pages: 213-247
  • DOI: 10.1016/bs.aivir.2020.08.001
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Presentation] Argonaute不要なクリ胴枯病菌での抗ウイルスRNA干渉機構 (2021)
  • Author(s): 佐藤有希代
  • Organizer: 令和3年度日本植物病理学会大会
• [Presentation] Proof of concept for the neo-viral lifestyle of yado-kari virus 1 being hosted by yado-nushi virus 1 (2020)
  • Author(s): Subha Das
  • Organizer: Virology Meeting for Early Career Researchers 2020
  • Invited
• [Presentation] 2A-mediated co-translational cleavage is prerequisite for the viability of yado-kari virus 1 hosted by yado-nushi virus 1. (2020)
  • Author(s): Subha Das
  • Organizer: The 39th Annual Meeting of the American Society for Virology 2020.
  • Int'l Joint Research
• [Presentation] Maintenance and loss of dispensable genomic segments of a polymyco-like capsidless fungal virus with an eleven-segmented RNA genome during its infection cycle. (2020)
  • Author(s): Yukiyo Sato
  • Organizer: The 39th Annual Meeting of the American Society for Virology 2020.
  • Int'l Joint Research
• [Presentation] In-tree behavior of diverse viruses infecting the chestnut blight fungus. (2020)
  • Author(s): Nobuhiro Suzuki
  • Organizer: The 39th Annual Meeting of the American Society for Virology 2020.
  • Int'l Joint Research
• [Book] ネオウイルス学（河岡義裕編）「ヤドカリ」「ヤドヌシ」と呼ばれるウイルスの共生関係 (2021)
  • Author(s): 鈴木信弘
  • Total Pages: 123-134
  • Publisher: 集英社
  • ISBN: 9784830041389
• [Book] “Fungal, invertebrate, bacterial archaeal viruses.” Encyclopedia of Virology 4th Edition (Ed. D. Bamford & M. Zuckerman). (2021)
  • Author(s): Suzuki, N., Krell, P., Feiss, M., and Prangishvili, M.
  • Total Pages: 1-972
  • Publisher: Elsevier
• [Book] An introduction to fungal viruses. Encyclopedia of Virology 4th Edition (Ed. D. Bamford & M. Zuckerman). (2021)
  • Author(s): Suzuki, N.
  • Total Pages: 431-442
  • Publisher: Elsevier
• [Book] Megabirnaviruses (Megabirnaviridae). Encyclopedia of Virology 4th Edition (Ed. D. Bamford & M. Zuckerman). (2021)
  • Author(s): Sato, Y., and Suzuki, N.
  • Total Pages: 594-600
  • Publisher: Elsevier
• [Book] Structure of dsRNA Mycoviruses. Encyclopedia of Virology 4th Edition (Ed. D. Bamford & M. Zuckerman). (2021)
  • Author(s): Caston, J. R., Suzuki, N., and Ghabrial, S. A.
  • Total Pages: 504-512
  • Publisher: Elsevier
• [Remarks] 植物・微生物相互作用グループ
  • URL: http://www.rib.okayama-u.ac.jp/pmi/index.html