Long-term preservation of fish genetic resources by cryopreservation and transplantation of germ-line stem cells

2019 Fiscal Year Final Research Report

• Project/Area Number: 17H01488
• Research Category: Grant-in-Aid for Scientific Research (A)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Aquatic life science
• Research Institution: Tokyo University of Marine Science and Technology
• Principal Investigator: Yoshizaki Goro 東京海洋大学, 学術研究院, 教授 (70281003)
• Co-Investigator(Kenkyū-buntansha): 山口 光太郎 埼玉県水産研究所, 水産研究所, 担当部長 (60502721)
• Project Period (FY): 2017-04-01 – 2020-03-31
• Keywords: タナゴ / トミヨ / 精原細胞 / 移植 / 凍結保存 / 緩慢凍結 / ガラス化

Outline of Final Research Achievements

Final goal of this study was to develop a method to preserve genetic resources of bitterlings and sticklebacks by combination of cryopreservation and transplantation of their germ-line stem cells. First, we optimized maturational stage of donor testes and recipient age used for the germ-cell transplantation. Second, we confirmed that production of gametes derived from several endangered Japanese bitterlings were possible by using surrogate Chinese rosy bitterlings. Also, cryopreservation of bitterling germ-line stem cells was successfully established.

Free Research Field

水族発生工学

Academic Significance and Societal Importance of the Research Achievements

本課題で構築した方法を用いることで、タナゴ類の遺伝子資源の半永久的な保存が可能になると期待される。特にRhodeus のみならずTanakia 属に属する種もタイリクバラタナゴを代理親に用いることで次世代生産が可能であったため、我が国に分布する多くの絶滅危惧種の生殖細胞を利用した遺伝子資源の保存が可能になると期待される。また本課題で開発した技術の多くは、他の絶滅危惧魚類へと応用することも可能であろう。