2019 Fiscal Year Annual Research Report
Anisotropic magnetic field effect imaging microspectroscopy: a technique to search cells for magnetic compass ability
| Project/Area Number |
17H03005
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| Research Institution | The University of Tokyo |
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Principal Investigator |
ウッドワード ジョナサン 東京大学, 大学院総合文化研究科, 教授 (80526054)
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| Project Period (FY) |
2017-04-01 – 2020-03-31
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| Keywords | 磁気受容 / ラジカル反応 / 磁場効果 / 蛍光顕微鏡 / スピン化学 |
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| Outline of Annual Research Achievements |
The anisotropic magnetic field microscope was fully tested and calibrated and can measure fluorescence images of single molecules. A static or oscillating (up to audio frequency) magnetic field can be delivered to the sample along any direction in 3D space and rotated in any plane. Careful testing has demonstrated that no artefactual responses due to the application of the field are observable in captured images or signals.
Various radical pair immobilization strategies have been tested and clear magnetic field effects are observed by tethering fluorophores to the surface of lysozymes. These proteins can be attached to the surface of glass slides allowing careful control of radical pair dynamics while keeping the pairs immobilized. This will finally allow the measurement of anisotropic magnetic field effects in the microscope.
A number of new radical pair generating photo reaction systems with very bright fluorophores have been developed. These systems should finally allow the detection of magnetic field effects on single radical pairs.
Simulations on single radical pairs exploiting a Monte Carlo approach with spin-state feedback were completed.
Studies of the magnetic field dependence of the photochemical reaction between flavins and hen egg white lysozyme have discovered the observation of a photopolymer product which traps fluorophores and results in a huge growth in fluorescence intensity. Changes in the magnetic field dependence provide track changes in the radical pair environment during the polymer formation.
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| Research Progress Status |
令和元年度が最終年度であるため、記入しない。
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| Strategy for Future Research Activity |
令和元年度が最終年度であるため、記入しない。
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| Remarks |
Research resulting from this work entitled "Cellular autofluorescence is magnetic field sensitive" is currently under review by the journal, Proceedings of the National Academy of Sciences (PNAS)
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