Epigenetic modification of sperm and its trans-generational changes.

2019 Fiscal Year Final Research Report

• Project/Area Number: 17H03680
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Developmental biology
• Research Institution: University of Yamanashi
• Principal Investigator: KOHDA Takashi 山梨大学, 大学院総合研究部, 教授 (60211893)
• Co-Investigator(Kenkyū-buntansha): 川崎 佑季 東京医科歯科大学, 難治疾患研究所, 助教 (60746890)
• Project Period (FY): 2017-04-01 – 2020-03-31
• Keywords: ヒドロキシメチルシトシン / シトシン修飾 / 精子

Outline of Final Research Achievements

We have been developing a new technique, Enzyme-assisted Identification of Genome Modification Assay (EnIGMA), which allows us to distinguish between mC and hmC at single-base resolution. In the present study, we applied this method to the analysis of cytosine modification in the mouse sperm genome and aim to identify the conditions that would alter the cytosine modification of the sperm genome.
To this end, we have successfully developed a new method, genome-wide EnIGMA-seq, that is capable of identification of cytosine modification status in the genome. In fact, we have shown that this new method is effective for comprehensive cytosine modification of the entire genome.

Free Research Field

エピジェネティクス

Academic Significance and Societal Importance of the Research Achievements

この研究で新たに開発したゲノムワイドEnIGMA-seq技術は、１塩基解像度でゲノムのシトシン修飾をメチルシトシン、ヒドロキシメチルシトシン、非修飾のシトシンの３つの状態のいずれであるかを同時に1分子上で開発することのできる手法であり、エピゲノム研究の新しい基盤技術として重要な意義を持つ。