Study of endogenous factors of nicotine detected in tea

2019 Fiscal Year Final Research Report

• Project/Area Number: 17H03756
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Crop production science
• Research Institution: Shizuoka University
• Principal Investigator: Morita Akio 静岡大学, 農学部, 教授 (20324337)
• Co-Investigator(Kenkyū-buntansha): 荻野 暁子 国立研究開発法人農業・食品産業技術総合研究機構, 果樹茶業研究部門, 主任研究員 (70370567) ; 古川 一実 沼津工業高等専門学校, 物質工学科, 准教授 (90353151) ; 一家 崇志 静岡大学, 農学部, 准教授 (90580647)
• Project Period (FY): 2017-04-01 – 2020-03-31
• Keywords: 茶 / ニコチン / 遺伝子組換え

Outline of Final Research Achievements

Tea nicotine biosynthesis may be regulated by a few genes. In addition, the activation of the polyamine synthesis pathway was associated with nicotine content, suggesting that there is another biosynthetic pathway for nicotine biosynthesis in tea plant that is not mediated by CsPMT.
It is important to control the catechin content in plants and to select strains that are resistant to the antimicrobial action of catechins, as well as to control the catechin content in plants. Furthermore, it was suggested that the transformation by bombardment method was more suitable for the transformation of tea plant.

Free Research Field

植物栄養学

Academic Significance and Societal Importance of the Research Achievements

茶で微量に検出されたニコチンについては，内因性由来であることをある程度証明できた．それらを直接的に証明するためには，まだ数ステップの研究が必要であるが，その実験系に必要な情報基盤について整備できた．特に，チャの遺伝子組換え植物体作出については，本研究だけでなく，茶業関連の機能性解析や将来の育種など，様々な研究への応用が期待できる．