Production of Subtype Specific Cardiomyocytes from Human iPSCs Based on Metabolism

2019 Fiscal Year Final Research Report

• Project/Area Number: 17H05067
• Research Category: Grant-in-Aid for Young Scientists (A)
• Allocation Type: Single-year Grants
• Research Field: General medical chemistry
• Research Institution: Keio University
• Principal Investigator: TOHYAMA Shugo 慶應義塾大学, 医学部(信濃町), 特任講師 (90528192)
• Project Period (FY): 2017-04-01 – 2020-03-31
• Keywords: ヒトiPS細胞 / 代謝 / 心筋細胞 / 移植 / 創薬

Outline of Final Research Achievements

The purpose of this study is to develop an efficient method to produce subtype specific cardiomyocytes from human iPSCs via metabolic regulation for regenerative medicine and drug discovery. In this project, we identified optimal culture conditions for human iPSCs expansion (1). In addition, we also developed an efficient method to produce subtype specific cardiomyocytes from human iPSCs (2). Moreover, we evaluated metabolic profiles in subtype specific cardiomyocytes via multi-omics (3). These findings will eventually promote the clinical applications of regenerative medicine and drug discovery.

Free Research Field

再生医学

Academic Significance and Societal Importance of the Research Achievements

本研究成果の学術的意義は、代謝プロファイルを基盤にヒトiPS細胞の増殖能をコントロールするだけでなく、高純度心室筋細胞と非心室筋細胞を作り分ける技術を開発することで、表現型のばらつきを解消する点である。表現型のばらつきを解消することで、安全かつ有効な再生医療の実現化および創薬への応用が期待できるため、社会的意義も極めて高いと考えられる。