Analysis of the bone marrow microenvironment regulating megakaryo/thrombopoiesis

2020 Fiscal Year Final Research Report

• Project/Area Number: 17H05073
• Research Category: Grant-in-Aid for Young Scientists (A)
• Allocation Type: Single-year Grants
• Research Field: Laboratory medicine
• Research Institution: Nagoya University
• Principal Investigator: Tamura Shogo 名古屋大学, 医学系研究科(保健), 講師 (60722626)
• Project Period (FY): 2017-04-01 – 2021-03-31
• Keywords: 骨髄微小環境

Outline of Final Research Achievements

This study suggested that a cellular origin of the bone marrow PDPN-expressing stromal cells discovered by the principal investigator is skeletal stem cells (SSCs). We analyzed the distribution of bone marrow PDPN-expressing stromal cells during bone and bone marrow development, especially in the secondary ossification center marrow formed at the epiphysis. We found that, during the formation of the secondary ossification center at the ends of bones, PDPN-positive cells invaded into the secondary ossification center along with periosteal vasculatures and populated the primary marrow. Furthermore, using an in vitro model, we found that PDPN-positive stromal cells maintained vascular homeostasis as a physiological function.

Free Research Field

血液学

Academic Significance and Societal Importance of the Research Achievements

本研究課題によって骨髄PDPN陽性間質細胞の細胞特性の一端を明らかにした。今後、本研究で得た知見をもとに骨髄PDPN陽性間質細胞特異的なin vivo遺伝子組み換えモデルを樹立することで、骨髄発生に対する骨髄PDPN陽性間質細胞の関与、そして骨髄PDPN陽性間質細胞が制御する骨髄環境構築メカニズムの解明が期待される 。