Elucidation of sleep/wakefulness regulation using forward genetic approach

2021 Fiscal Year Annual Research Report

• Project/Area Number: 17H06095
• Research Institution: University of Tsukuba
• Principal Investigator: 柳沢 正史 筑波大学, 国際統合睡眠医科学研究機構, 教授 (20202369)
• Co-Investigator(Kenkyū-buntansha): 船戸 弘正 筑波大学, 国際統合睡眠医科学研究機構, 客員教授 (90363118) ; Liu Qinghua 筑波大学, 国際統合睡眠医科学研究機構, 教授 (90723792)
• Project Period (FY): 2017-04-25 – 2022-03-31
• Keywords: 睡眠覚醒 / 脳神経 / 遺伝子改変マウス / ゲノム編集 / 細胞内シグナル伝達系

Outline of Annual Research Achievements

睡眠は動物に普遍的に認められる行動であり、その不調は心身の疾患リスクを高める。しかし、睡眠覚醒行動を制御する神経科学的メカニズムは不明である。本研究は、睡眠のフォワード・ジェネティクス研究により新規睡眠制御分子を同定するとともに、リン酸化酵素SIK3およびリークチャネルNALCNによる睡眠覚醒制御機構解明を目指している。前年度に引き続き脳波筋電図にもとづくランダム点突然変異マウスの睡眠異常スクリーニングを行い遺伝性に睡眠異常を示す個体の同定、睡眠表現型の遺伝性の確認、連鎖解析及び全エクソームシーケンスによる責任遺伝子変異の同定を進めた。同定された遺伝子についてはAAV-PHP.eBを用いたり、CRISPR法により遺伝子改変マウスを作成して因果性の確認を行っている。SIK３変異を標的としたサプレッサースクリーニングも進めており、SIK3遺伝子変異が存在してもノンレム睡眠量が正常化する家系を樹立している。SIK3パスウェイを構成する遺伝子を操作したマウスを過眠モデルとして用いてマルチオミクス解析を行っている。メタボローム解析によって睡眠を促進する効果を持つメタボライトを同定した。プロテオーム、トランスクリプトーム解析も並行して実施し、異なる過眠モデル脳で共通した変化を示す分子を同定した。陽イオンリークチャネルNALCNのニューロンタイプ特異的なレム断片化DRL変異誘導や機能欠失によって多彩なレム睡眠異常を呈することが明らかとなった。

Research Progress Status

令和3年度が最終年度であるため、記入しない。

Strategy for Future Research Activity

令和3年度が最終年度であるため、記入しない。

Research Products

• [Journal Article] Spectroscopic second and third harmonic generation microscopy using a femtosecond laser source in the third near-infrared (NIR-III) optical window (2022)
  • Author(s): Yusuke Murakami, Minori Masaki, Shinichi Miyazaki, Ryosuke Oketani, Yu Hayashi, Masashi Yanagisawa, Sakiko Honjoh, Hideaki Kano
  • Journal Title: Biomedical Optics Express Volume: 13 Pages: 694-708
  • DOI: 10.1364/BOE.446273
  • Peer Reviewed / Open Access
• [Journal Article] Morning preference is associated with subjective happiness among Japanese female workers: A moderation analysis by sleep characteristics from the SLEPT study (2022)
  • Author(s): Daisuke Hori, Yo Arai, Emi Morita, Yu Ikeda, Kei Muroi, Mami Ishitsuka, Tomohiko Ikeda, Tsukasa Takahashi, Shotaro Doki, Yuichi Oi, Shinichiro Sasahara, Asuka Ishihara, Sumire Matsumoto, Takashi Kanbayashi, Masashi Yanagisawa, Mokoto Satoh, Ichiyo Matsuzaki
  • Journal Title: Chronobiology International Volume: 39 Pages: 690-703
  • DOI: 10.1080/07420528.2022.2028801
  • Peer Reviewed / Open Access
• [Journal Article] Detecting cell assemblies by NMF-based clustering from calcium imaging data (2022)
  • Author(s): Mizuo Nagayama, Toshimitsu Aritake, Hideitsu Hino, Takeshi Kanda, Takehiro Miyazaki, Masashi Yanagisawa, Shotaro Akaho, Noboru Murata
  • Journal Title: Neural Networks Volume: 149 Pages: 29-39
  • DOI: 10.1016/j.neunet.2022.01.023
  • Peer Reviewed / Open Access
• [Journal Article] Effect of sevoflurane preconditioning on sleep reintegration after alteration by lipopolysaccharide (2022)
  • Author(s): Tsuyoshi Nemoto, Yoko Irukayama-Tomobe, Yuki Hirose, Hiromu Tanaka, Genki Takahashi, Satoshi Takahashi, Masashi Yanagisawa, Takashi Kanbayashi
  • Journal Title: Journal of Sleep Research Volume: 0 Pages: e13556
  • DOI: 10.1111/jsr.13556
  • Peer Reviewed / Open Access
• [Journal Article] Design and synthesis of novel orexin 2 receptor agonists based on naphthalene skeleton (2022)
  • Author(s): Tsubasa Hino, Tsuyoshi Saitoh, Yasuyuki Nagumo, Naoshi Yamamoto, Noriki Kutsumura, Yoko Irukayama-Tomobe, Yukiko Ishikawa, Ryuji Tanimura, Masashi Yanagisawa, Hiroshi Nagase
  • Journal Title: Bioorganic & Medicinal Chemistry Letters Volume: 59 Pages: 128530
  • DOI: 10.1016/j.bmcl.2022.128530
  • Peer Reviewed / Open Access
• [Journal Article] Essential structure of orexin 1 receptor antagonist YNT-707: Conversion of the 16-cyclopropylmethyl group to the 16-sulfonamide group in d-nor-nalfurafine derivatives (2022)
  • Author(s): Koki Katoh, Noriki Kutsumura, Naoshi Yamamoto, Yasuyuki Nagumo, Tsuyoshi Saitoh, Yukiko Ishikawa, Yoko Irukayama-Tomobe, Ryuji Tanimura, Masashi Yanagisawa, Hiroshi Nagase
  • Journal Title: Bioorganic & Medicinal Chemistry Letters Volume: 59 Pages: 128550
  • DOI: 10.1016/j.bmcl.2022.128550
  • Peer Reviewed / Open Access
• [Journal Article] Effect of removal of the 14-hydroxy group on the affinity of the 4,5-epoxymorphinan derivatives for orexin and opioid receptors (2022)
  • Author(s): Koki Katoh, Naoshi Yamamoto, Yukiko Ishikawa, Yoko Irukayama-Tomoebe, Ryuji Tanimura, Tsuyoshi Saitoh, Yasuyuki Nagumo, Noriki Kutsumura, Masashi Yanagisawa, Hiroshi Nagase
  • Journal Title: Bioorganic & Medicinal Chemistry Letters Volume: 59 Pages: 128527
  • DOI: 10.1016/j.bmcl.2022.128527
  • Peer Reviewed / Open Access
• [Journal Article] Discovery of orexin 2 receptor selective and dual orexin receptor agonists based on the tetralin structure: Switching of receptor selectivity by chirality on the tetralin ring (2022)
  • Author(s): Keita Iio, Tsuyoshi Saitoh, Ryuichiro Ohshita, Tsubasa Hino, Mao Amezawa, Yoshiaki Takayama, Yasuyuki Nagumo, Naoshi Yamamoto, Noriki Kustumura, Yoko Irukayama-Tomobe, Yukiko Ishikawa, Ryuji Tanimura, Masashi Yanagisawa, Hiroshi Nagase
  • Journal Title: Bioorganic & Medicinal Chemistry Letters Volume: 60 Pages: 128555
  • DOI: 10.1016/j.bmcl.2022.128555
  • Peer Reviewed / Open Access
• [Journal Article] Genetic Inactivation of Free Fatty Acid Receptor 3 Impedes Behavioral Deficits and Pathological Hallmarks in the APP swe Alzheimer's Disease Mouse Model (2022)
  • Author(s): Marta Zamarbide, Eva Martinez-Pinilla, Francisco Gil-Bea, Masashi Yanagisawa, Rafael Franco, Alberto Perez-Mediavilla
  • Journal Title: International Journal of Molecular Sciences Volume: 23 Pages: 3553
  • DOI: 10.3390/ijms23073533
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Two novel mouse models mimicking minor deletions in 22q11.2 deletion syndrome revealed the contribution of each deleted region to psychiatric disorders (2021)
  • Author(s): Ryo Saito, Chika Miyoshi, Michinori Koebis, Itaru Kushima, Kazuki Nakao, Daisuke Mori, Norio Ozaki, Hiromasa Funato, Masashi Yanagisawa, Atsu Aiba
  • Journal Title: Mlecular Brain Volume: 14 Pages: 68
  • DOI: 10.1186/s13041-021-00778-7
  • Peer Reviewed / Open Access
• [Journal Article] Posterior subthalamic nucleus (PSTh) mediates innate fear-associated hypothermia in mice (2021)
  • Author(s): Can Liu, Chia-Ying Lee, Greg Asher, Liqin Cao, Yuka Terakoshi, Peng Cao, Reiko Kobayakawa, Ko Kobayakawa, Katsuyasu Sakurai, Qinghua Liu
  • Journal Title: Nature Communications Volume: 12 Pages: 2648
  • DOI: 10.1038/s41467-021-22914-6
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Metabolic responses to polychromatic LED and OLED light at night (2021)
  • Author(s): Asuka Ishihara, Insung Park, Yoko Suzuki, Katsuhiko Yajima, Huiyun Cui, Masashi Yanagisawa, Takeshi Sano, Junji Kido, Kumpei Tokuyama
  • Journal Title: Scientific Reports Volume: 11 Pages: 12402
  • DOI: 10.1038/s41598-021-91828-6
  • Peer Reviewed / Open Access
• [Journal Article] O-EG004. Comparing the accuracy of sleep staging data from a new wearable sleep electroencephalography device versus the Fitbit Charge 3 with polysomnography as reference (2021)
  • Author(s): Yoko Suzuki, Takashi Abe, Fusae Kawana, Satomi Okabe, Toshio Kokubo, Kazuya Hoshino, Misao Baba, Masaaki Fujiwara, Masashi Yanagisawa
  • Journal Title: Clinical Neurophysiology Volume: 132 Pages: 67
  • DOI: 10.1016/j.clinph.2021.02.125
  • Peer Reviewed / Open Access
• [Journal Article] Cerebral capillary blood flow upsurge during REM sleep is mediated by A2a receptors (2021)
  • Author(s): Chia-Jung Tsai, Takeshi Nagata, Chih-Yao Liu, Takaya Suganuma, Takeshi Kanda, Takehiro Miyazaki, Kai Liu, Tsuyoshi Saitoh, Hiroshi Nagase, Michael Lazarus, Kaspar E. Vogt, Masashi Yanagisawa, Yu Hayashi
  • Journal Title: Cell Reports Volume: 36 Pages: 109558
  • DOI: 10.1016/j.celrep.2021.109558
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] The Role of reproductive hormones in sex differences in sleep homeostasis and arousal response in mice (2021)
  • Author(s): Choi Jinhwan, Kim Staci J., Fujiyama Tomoyuki, Miyoshi Chika, Park Minjeong, Suzuki-Abe Haruka, Yanagisawa Masashi, Funato Hiromasa
  • Journal Title: Frontiers in Neuroscience Volume: 15 Pages: 739236
  • DOI: 10.3389/fnins.2021.739236
  • Peer Reviewed / Open Access
• [Journal Article] GI-Sleep Net:A Highly Versatile Image－Based Sleep Classification Using a Deep Learning Algorithm (2021)
  • Author(s): Tianxiang Gao, Jiayi Li, Yuji Watanabe, Cijung Hung, Akihiro Yamanaka, Kazumasa Horie, Masashi Yanagisawa, Masahiro Ohsawa, Kazuhiko Kume
  • Journal Title: Clocks & Sleep Volume: 3 Pages: 581-597
  • DOI: 10.3390/clockssleep3040041
  • Peer Reviewed / Open Access
• [Journal Article] Metabolomic and pharmacologic analyses of brain substances associated with sleep pressure in mice (2021)
  • Author(s): Haruka Suzuki-Abe, Kazuhiro Sonomura, Shinya Nakata, Kazuya Miyanishi, Asmaa Mahmoud, Noriko Hotta-Hirashima, Chika Miyoshi, Taka-Aki Sato, Hiromasa Funato, Masashi Yanagisawa
  • Journal Title: Neuroscience Research Volume: 177 Pages: 16-24
  • DOI: 10.1016/j.neures.2021.11.008
  • Peer Reviewed / Open Access
• [Journal Article] Protocol for sleep analysis in the brain of genetically modified adult mice (2021)
  • Author(s): Kanako Iwasaki, Noriko Hotta-Hirashima, Hiromasa Funato, Masashi Yanagisawa
  • Journal Title: STAR Protocols Volume: 2 Pages: 100982
  • DOI: 10.1016/j.xpro.2021.100982
  • Peer Reviewed / Open Access
• [Presentation] 脳状態と脳機能を結ぶ大脳皮質局所機能的ネットワークのダイナミクス (2022)
  • Author(s): 上田壮志
  • Organizer: 行動生理学 特別セミナー
• [Presentation] 睡眠の謎に挑む～原理の追求から社会実装まで～ (2022)
  • Author(s): 柳沢正史
  • Organizer: 山梨医学フォーラム,山梨大学
  • Invited
• [Presentation] 睡眠の謎に挑む～『眠気』の実体を求めて～ (2022)
  • Author(s): 柳沢正史
  • Organizer: 筑波大学生命科学動物資源センター 20周年記念シンポジウム,筑波大学
  • Invited
• [Presentation] 睡眠の謎に挑む～『眠気』の実体を求めて～ (2022)
  • Author(s): 柳沢正史
  • Organizer: 第95回日本薬理学会年会,福岡国際会議場
  • Int'l Joint Research / Invited
• [Presentation] レム睡眠制御の分子機構：近位依存性ビオチン標識法BioIDを用いた電位非依存性陽イオンチャネルNALCNのタンパク質間相互作用因子の探索 (2022)
  • Author(s): 藤山知之, 山元ひかり, 北園智弘, 一久綾, 管野里美, 柿﨑美代, Choi Jinhwan, 中田慎也, 村田知弥, 水野聖哉, 杉山文博, 高橋智, 船戸弘正, 柳沢正史
  • Organizer: 第95回日本薬理学会年会
  • Int'l Joint Research
• [Presentation] Distinct network structures emerge in the primary motor cortex during active and quiet wake (2022)
  • Author(s): Takeshi Kanda, Takehiro Miyazaki, Kotaro Sakamoto, Hideitsu hino, Masashi Yanagisawa
  • Organizer: 第99回日本生理学会
  • Int'l Joint Research
• [Presentation] Establishment of a Model Mouse Pedigree with hypersomnia and obesity by EEG/EMG-Based Forward Genetic Screening (2022)
  • Author(s): 三好千香, 堀田範子, 管野里美, 一久綾, 柿﨑美代, 柳沢正史, 船戸弘正
  • Organizer: 第99回日本生理学会
  • Int'l Joint Research
• [Presentation] Loss of canonical Hdac4 signaling leads to dysregulated sleep (2022)
  • Author(s): Staci J. Kim
  • Organizer: 第99回日本生理学会 JST-CREST "Opt Bio" /WPI-IIIS Joint Symposium
  • Int'l Joint Research
• [Presentation] NALCN in the forebrain and pons-medulla regions have distinct roles in REM sleep regulation (2022)
  • Author(s): Tomoyuki Fujiyama, Satomi Kanno, Noriko Hirashima Hotta, Miyo Kakizaki, Aya Ikkyu, Yosuke Tsuneoka, Manabu Abe, Ayako Mochizuki, Patricia Seoane-Collazo, Takehiro Miyazaki, Seiya Mizuno, Chika Miyoshi, Takeshi Kanda, Masahiko Watanabe, Kenji Sakimura, Satoru Takahashi, Hiromasa Funato, Masashi Yanagisawa
  • Organizer: 第99回日本生理学会JST-CREST "Opt Bio" /WPI-IIIS Joint Symposium
  • Int'l Joint Research
• [Presentation] Natural history study of sleep disturbances in CDKL5 deficiency disorder mice (2022)
  • Author(s): Cao Liqin, Tingting Lou, Xin Zhang, Jing Ma, Zhiqiang Wang, Teruyuki Tanaka, Hiromasa Funato, Qinghua Liu, Masashi Yanagisawa
  • Organizer: 第99回日本生理学会JST-CREST "Opt Bio" /WPI-IIIS Joint Symposium
  • Int'l Joint Research
• [Presentation] Learning and memory deficit in adult dreamless mice (2022)
  • Author(s): Deependra Kumar, Tomoyuki Fujiyama, Takeshi Kanda, Chika Miyoshi, Miyo Kakizaki, Masanori Sakaguchi, Hiromasa Funato, Masashi Yanagisawa
  • Organizer: 第99回日本生理学会JST-CREST "Opt Bio" /WPI-IIIS Joint Symposium
  • Int'l Joint Research
• [Presentation] Pre-narcoleptics are more prone to suvorexant-induced cataplexy. (2022)
  • Author(s): Mahesh K. Kaushik, Kosuke Aritake, Yoan Cherasse, Aya Imanishi, Takeshi Kanbayashi, Yoshihiro Urade, Masashi Yanagisawa
  • Organizer: 第99回日本生理学会JST-CREST "Opt Bio" /WPI-IIIS Joint Symposium
  • Int'l Joint Research
• [Presentation] Orexin receptor antagonists ameliorate the symptoms of REM sleep behavior disorder in a novel mouse model and in human patients (2022)
  • Author(s): Mari Hondo, Takuro Endo, Ayako Mochizuki, Masanori Dantsuji, Takeshi Kanda, Makito Sato, Fusae Kawana, Yukiko Ishikawa, Kazue Suenaga, Shiro Nakamura, Manabu Abe, Tomio Inoue, Kenji Sakimura, Hiromasa Funato, Takeshi Sakurai, Masashi Yanagisawa
  • Organizer: 第99回日本生理学会JST-CREST "Opt Bio" /WPI-IIIS Joint Symposium
  • Int'l Joint Research
• [Presentation] Sleep/wakefulness and body weight growth from infancy to adulthood in a hypersomnia model, Sleepy mutant mouse. (2022)
  • Author(s): Noriko Hotta-Hirashima, Chika Miyoshi, Aya Ikkyu, Miyo Kakizaki, Satomi Kanno, Kumi Ebihara, Hiromasa Funato, Masashi Yanagisawa
  • Organizer: 第99回日本生理学会JST-CREST "Opt Bio" /WPI-IIIS Joint Symposium
  • Int'l Joint Research
• [Presentation] The novel intracellular signaling pathways for the regulation of homeostatic sleep need (2022)
  • Author(s): Tomohiro Kitazono, Noriko Hotta-Hirashima, Kanako Iwasaki, Tomoyuki Fujiyama, Hiromasa Funato, Masashi Yanagisawa
  • Organizer: 第99回日本生理学会JST-CREST "Opt Bio" /WPI-IIIS Joint Symposium
  • Int'l Joint Research
• [Presentation] SIK3 in different hypothalamic areas mediates whole-body energy balance (2022)
  • Author(s): Patricia Seoane-Collazo, Masashi Yanagisawa, Hiromasa Funato
  • Organizer: 第99回日本生理学会JST-CREST "Opt Bio" /WPI-IIIS Joint Symposium
  • Int'l Joint Research
• [Presentation] Sik3 regulates sleep need via glutamatergic neurons in cerebral cortex (2022)
  • Author(s): Fuyuki Asano, Tomoyuki Fujiyama, Chika Miyoshi, Tomohiro Kitazono, Noriko Hotta-Hirashima, Miyo Kakizaki, Aya Ikkyu, Satomi Kanno, Shinya Nakata, Nodoka Asama, Seiya Mizuno, Fumihiro Sugiyama, Satoru Takahashi, Hiromasa Funato, Masashi Yanagisawa
  • Organizer: 第99回日本生理学会JST-CREST "Opt Bio" /WPI-IIIS Joint Symposium
  • Int'l Joint Research
• [Presentation] Where does Sleepy mutation of SIK3 cause sleep phenotypes? (2022)
  • Author(s): Kanako Iwasaki, Tomoyuki Fujiyama, Shinya Nakata, Minjeong Park, Chika Miyoshi, Noriko Hotta-Hirashima, Aya Ikkyu, Miyo Kakizaki, Yukiko Ishikawa, Fumihiro Sugiyama, Seiya Mizuno, Manabu Abe, Kenji Sakimura, Satoru Takahashi, Hiromasa Funato, Masashi Yanagisawa
  • Organizer: 第99回日本生理学会JST-CREST "Opt Bio" /WPI-IIIS Joint Symposium
  • Int'l Joint Research
• [Presentation] Sleep/wake behavior of mice lacking PKA phosphorylation site in SIK3 (2022)
  • Author(s): Minjeong Park, Takato Honda, Chika Miyoshi, Tomoyuki Fujiyama, Miyo Kakizaki, Aya Ikkyu, Seiya Mizuno, Satoru Takahashi, Hiromasa Funato, Masashi Yanagisawa
  • Organizer: 第99回日本生理学会JST-CREST "Opt Bio" /WPI-IIIS Joint Symposium
  • Int'l Joint Research
• [Presentation] Molecular mechanisms for SIK3(Sleepy)-mediated sleep/wake regulation (2022)
  • Author(s): Shinya Nakata, Haruna Abe-Komiya, Tomoyuki Fujiyama, Chika Miyoshi, Aya Ikkyu, Fuyuki Asano, Seiya Mizuno, Fumihiro Sugiyama, Satoru Takahashi, Hiromasa Funato, Masashi Yanagisawa
  • Organizer: 第99回日本生理学会JST-CREST "Opt Bio" /WPI-IIIS Joint Symposium
  • Int'l Joint Research
• [Presentation] OX2R-selective orexin agonism is sufficient to suppress narcoleptic symptoms, cataplexy and wake fragmentation, without inducing drug-seeking behavior in mouse models (2022)
  • Author(s): Hikari Yamamoto, Yasuyuki Nagumo, Yukiko Ishikawa, Yoko Irukayama-Tomobe, Tsuyoshi Nemoto, Hiromu Tanaka, Genki Takahashi, Akihisa Tokuda, Tsuyoshi Saitoh, Hiroshi Nagase, Hiromasa Funato, Masashi Yanagisawa
  • Organizer: 第99回日本生理学会JST-CREST "Opt Bio" /WPI-IIIS Joint Symposium
  • Int'l Joint Research
• [Presentation] Automatic assessment of sleep recordings from a portable IoT EEG device. (2022)
  • Author(s): Juan Carlos Neira, Leo Ota, Kazumasa Horie, Masashi Yanagisawa, Hiroyuki Kitagawa
  • Organizer: 第99回日本生理学会JST-CREST "Opt Bio" /WPI-IIIS Joint Symposium
  • Int'l Joint Research
• [Presentation] Metabolomic and pharmacologic analyses of brain substances associated with sleep pressure in mice (2022)
  • Author(s): Haruka Suzuki-Abe, Kazuhiro Sonomura, Shinya Nakata, Kazuya Miyanishi, AsmaaMahmoud, Noriko Hotta-Hirashima, Chika Miyoshi, Taka-Aki Sato, Hiromasa Funato, Masashi Yanagisawa
  • Organizer: 第99回日本生理学会JST-CREST "Opt Bio" /WPI-IIIS Joint Symposium
  • Int'l Joint Research
• [Presentation] Sleepy mouse as a model of idiopathic hypersomnia (2022)
  • Author(s): Asmaa Elhosainy Mohamed Mahmoud, Haruka Suzuki-Abe, Mahesh Kaushik, Staci Jakyong Kim, Tsuyoshi Saitoh, Yukiko Ishikawa, Noriko HottaHirashima, Chika Miyoshi, Hiromasa Funato, Masashi Yanagisawa
  • Organizer: 第99回日本生理学会JST-CREST "Opt Bio" /WPI-IIIS Joint Symposium
  • Int'l Joint Research
• [Presentation] 睡眠覚醒の謎に挑む～原理の追求から社会実装まで～ (2022)
  • Author(s): 柳沢正史
  • Organizer: 第49回日本集中治療医学会学術集会,仙台国際センター
  • Int'l Joint Research / Invited
• [Presentation] Deciphering the mystery of sleep: toward the molecular substrate for "sleepiness" (2022)
  • Author(s): 柳沢正史
  • Organizer: 2nd China Narcolepsy and International Hypersomnolence Meeting,Chinese Sleep Research Society
  • Int'l Joint Research / Invited
• [Presentation] Young Neurons Role in Consolidating Memory during REM Sleep (2022)
  • Author(s): Deependra Kumar
  • Organizer: Annual Symposium of the Federation of Indian Physiological Society 2022 (FIPS-22) International Virtual Conference
  • Int'l Joint Research
• [Presentation] 睡眠覚醒の謎に挑む ～原理の追求から社会実装まで～ (2021)
  • Author(s): 柳沢正史
  • Organizer: （オンライン）ACT-541468 Investigators Meeting(Daridorexant)
  • Invited
• [Presentation] Toward the Molecular Basis of Sleepness (2021)
  • Author(s): 柳沢正史
  • Organizer: (オンライン)ASSM2021
  • Int'l Joint Research / Invited
• [Presentation] 睡眠覚醒の謎に挑む (2021)
  • Author(s): 柳沢正史
  • Organizer: (オンライン)第68回日本実験動物学会総会
  • Int'l Joint Research / Invited
• [Presentation] 睡眠覚醒の謎に挑む (2021)
  • Author(s): 柳沢正史
  • Organizer: 第62回日本神経学会学術大会, 国立京都国際会館
  • Int'l Joint Research / Invited
• [Presentation] 睡眠覚醒の謎に挑む (2021)
  • Author(s): 柳沢正史
  • Organizer: (オンライン)第68回日本麻酔科学会学術集会
  • Invited
• [Presentation] 睡眠覚醒の謎に挑む～原理の追求から社会実装まで～ (2021)
  • Author(s): 柳沢正史
  • Organizer: (オンライン)第32回日本老年学会総会
  • Int'l Joint Research / Invited
• [Presentation] 睡眠と覚醒 (2021)
  • Author(s): 柳沢正史
  • Organizer: (オンライン)慶應義塾大学医学部医学部生理学特別講義
  • Invited
• [Presentation] 睡眠覚醒の謎に挑む (2021)
  • Author(s): 柳沢正史
  • Organizer: 令和３年金沢大学十全医学会総会・学術集会,金沢大学十全講堂
  • Invited
• [Presentation] 睡眠覚醒の謎に挑む (2021)
  • Author(s): 柳沢正史
  • Organizer: 第48回日本毒性学会学術年会, 神戸国際会議場
  • Int'l Joint Research / Invited
• [Presentation] 恒常的睡眠要求を制御する新規シグナル伝達経路の探索 (2021)
  • Author(s): 北園智弘, 堀田範子, 岩崎加奈子, 藤山知之, 船戸弘正, 柳沢正史
  • Organizer: 第44回日本神経科学大会・CJK第1回国際会議
  • Int'l Joint Research
• [Presentation] The impact of social environment on sleep architecture in mice (2021)
  • Author(s): Minjeong Park, 櫻井勝康, 船戸弘正, 柳沢正史
  • Organizer: 第44回日本神経科学大会・CJK第1回国際会議
  • Int'l Joint Research
• [Presentation] Endogenous SIK3 regulates sleep need through glutamatergic neurons in cerebral cortex (2021)
  • Author(s): 浅野冬樹, 藤山智之, 三好千香,北園智弘, 堀田範子, 柿﨑美代, 一久綾, 管野里美, 中田慎也, 浅間のどか, 水野聖哉, 杉山文博, 高橋智, 船戸弘正, 柳沢正史
  • Organizer: 第44回日本神経科学大会・CJK第1回国際会議
  • Int'l Joint Research
• [Presentation] Neuroanatomical and biochemical analysis of how the Sleepy mutant of SIK3 increases sleep (2021)
  • Author(s): 岩崎加奈子, 藤山智之, 上田壮志, 中田慎也, Minjeong Park, 三好千香, 堀田範子, 一久綾, 柿﨑美代, 杉山文博, 水野聖哉, 阿部学, 崎村健司, 高橋智, 松田道行, 船戸弘正, 柳沢正史
  • Organizer: 第44回日本神経科学大会
  • Int'l Joint Research
• [Presentation] Phosphorylation-deficient Sleepy2 mutant mice exhibited decreased NREM sleep (2021)
  • Author(s): 浅間のどか, Staci J. Kim, 藤山智之, 浅野冬樹, 三好千香, 堀田範子, 柿﨑美代, 一久綾, 管野里美, 中田慎也, 水野聖哉, 高橋智, 船戸弘正, 柳沢正史
  • Organizer: 第44回日本神経科学大会・CJK第1回国際会議
  • Int'l Joint Research
• [Presentation] The maturation of sleep homeostasis during development (2021)
  • Author(s): 片山璃沙子, 堀田範子, 三好千香, 一久綾, 管野里美, 海老原恭美, 船戸弘正, 柳沢正史
  • Organizer: 第44回日本神経科学大会・CJK第1回国際会議
  • Int'l Joint Research
• [Presentation] Loss of Sleepy2 in Foxd1 lineage of cells leads to gross abnormality in skull and growth retardation (2021)
  • Author(s): Staci Jakyong Kim , 三好千香, 堀田範子, 管野里美, 柿﨑美代, 一久綾, 船戸弘正, 柳沢正史
  • Organizer: （オンライン）第44回日本神経科学大会・CJK第1回国際会議
  • Int'l Joint Research
• [Presentation] The functional analysis of SIK3 kinase activity for sleep/wake regulation (2021)
  • Author(s): 中田慎也, 小宮春奈, 藤山智之, 三好千香, 一久綾, 浅野冬樹, 水野聖哉, 杉山文博, 高橋智, 船戸弘正, 柳沢正史
  • Organizer: 第44回日本神経科学大会・CJK第1回国際会議
  • Int'l Joint Research
• [Presentation] 睡眠の謎に挑む～原理の追求から社会実装まで～ (2021)
  • Author(s): 柳沢正史
  • Organizer: (オンライン)AMED 再生・細胞医療・遺伝子治療研究交流会
  • Invited
• [Presentation] Similar structures emerge in local cortical networks during quiet wake and NREM sleep (2021)
  • Author(s): 上田壮志, 宮崎峻弘, 坂本航太郎, 日野英逸, 柳沢正史
  • Organizer: 第31回日本神経回路学会
  • Int'l Joint Research
• [Presentation] 睡眠多様性：最先端の神経科学『ノンレム睡眠を制御する細胞内シグナル伝達系の解明』 (2021)
  • Author(s): 柳沢正史, 林悠, Sam Reiter, 乘本裕明, 船戸弘正, 長谷川恵美
  • Organizer: 日本睡眠学会第46回定期学術集会,福岡国際会議場
  • Int'l Joint Research / Invited
• [Presentation] Molecular mechanisms for SIK3(Sleepy)-mediated sleep/wake regulation (2021)
  • Author(s): Shinya Nakata, Haruna Abe-Komiya, Tomoyuki Fujiyama, Chika Miyoshi, Aya Ikkyu, Fuyuki Asano, Seiya Mizuno, Fumihiro Sugiyama, Satoru Takahashi, Hiromasa Funato, Masashi Yanagisawa
  • Organizer: Tsukuba Conference 2021（筑波会議2021）
  • Int'l Joint Research
• [Presentation] Advanced techniques in neuroscience research (Optogenetics/neural imaging/unit recording) (2021)
  • Author(s): Deependra Kumar
  • Organizer: Academic Interaction with student in department of Physiology AIIMS, New Delhi.
  • Int'l Joint Research
• [Presentation] Deciphering the mystery of sleep: toward the molecular substrate for sleepiness (2021)
  • Author(s): 柳沢正史
  • Organizer: Brain States and Beyond,Center for Translational Neuromedicine,University of Copenhagen
  • Int'l Joint Research / Invited
• [Presentation] Deciphering the Mystery of Sleep: From the Discovery of Orexin to Forward Genetics in Mice (2021)
  • Author(s): 柳沢正史
  • Organizer: Orexin Science Summit 2021,Idorsia Pharmaceuticals Ltd.
  • Int'l Joint Research / Invited
• [Presentation] 睡眠覚醒の謎に挑む～マウスforward geneticsを武器として～ (2021)
  • Author(s): 柳沢正史
  • Organizer: 理化学研究所バイオリソース研究センター設立20周年記念シンポジウム,理化学研究所
  • Invited
• [Presentation] 「眠気」を制御する新規シグナル伝達経路の探索と解析 (2021)
  • Author(s): 北園智弘
  • Organizer: 第94回日本生化学大会
  • Int'l Joint Research
• [Presentation] Perturbation in Hdac4 signaling leads to dysregulated NREMS (2021)
  • Author(s): Staci J. Kim, Noriko Hotta-Hirashima, Nodoka Asama, Taeko Matsuoka, Tomoyuki Fujiyama, Aya Ikkyu, Miyo Kakizaki, Satomi Kanno, Sseiya Mizuno, Satoru Takahashi, Chika Miyoshi, Hiromasa Funato, Masashi Yanagisawa
  • Organizer: SFN Neuroscience 2021 50th Annual Meeting participation
  • Int'l Joint Research
• [Presentation] Involvement of microglia in sleep/wake regulation under acute social defeat stress in mice (2021)
  • Author(s): Kazuya Miyanishi, Hiromasa Funato, Masashi Yanagisawa
  • Organizer: NEUROSCIENCE 2021
  • Int'l Joint Research
• [Presentation] Special Lecture - The Mysteries of Sleep - Toward the Molecular Substrate for "Sleepiness" (2021)
  • Author(s): 柳沢正史
  • Organizer: Sosiety for Neuroscience 2021,SfN
  • Int'l Joint Research / Invited
• [Presentation] Where does Sleepy mutation of SIK3 increase non-REM sleep? (2021)
  • Author(s): Kanako Iwasaki, Tomoyuki Fujiyama, Shinya Nakata, Minjeong Park, Chika Miyoshi, Noriko Hotta-Hirashima, Aya Ikkyu, Miyo Kakizaki, Yukiko Ishikawa, Fumihiro Sugiyama, Seiya Mizuno, Manabu Abe, Kenji Sakimura, Satoru Takahashi, Hiromasa Funato, Masashi Yanagisawa
  • Organizer: NEUROSCIENCE 2021
  • Int'l Joint Research
• [Presentation] Sik3 regulates sleep need via glutamatergic neurons in cerebral cortex in mice (2021)
  • Author(s): Fuyuki Asano, Tomoyuki Fujiyama, Chika Miyoshi, Tomohiro Kitazono, Noriko Hotta-Hirashima, Miyo Kakizaki, Aya Ikkyu, Satomi Kanno, Shinya Nakata, Nodoka Asama, Seiya Mizuno, Fumihiro Sugiyama, Satoru Takahashi, Hiromasa Funato, Masashi Yanagisawa
  • Organizer: NEUROSCIENCE 2021
  • Int'l Joint Research
• [Presentation] Why is REM sleep Important? (2021)
  • Author(s): Deependra Kumar
  • Organizer: 12th Indian Scientists Association in Japan (ISAJ) symposium
  • Int'l Joint Research
• [Presentation] 『眠気』の正体を求めて (2021)
  • Author(s): 柳沢正史
  • Organizer: 第44回日本分子生物学会,パシフィコ横浜
  • Int'l Joint Research / Invited
• [Presentation] Molecular mechanisms of REM sleep regulation: identifying protein-protein interactions of NALCN channel through BioID technique (2021)
  • Author(s): Hikari Yamamoto, Tomoyuki Fujiyama, Tomohiro Kitazono, Aya Ikkyu, Satomi Kanno, Miyo Kakizaki, Choi Jinhwan, Shinya Nakata, Kazuya Murata, Seiya Mizuno, Fumihiro Sugiyama, Satoru Takahashi, Hiromasa Funato, Masashi Yanagisawa
  • Organizer: 第44回日本分子生物学会年会
  • Int'l Joint Research
• [Presentation] 睡眠の謎に挑む～原理の追求から社会実装まで～ (2021)
  • Author(s): 柳沢正史
  • Organizer: 第49回獨協医学学術集会,獨協大学
  • Invited
• [Presentation] 潜在的睡眠改善食品の新規睡眠イメージング技術による探索 (2021)
  • Author(s): 上田壮志
  • Organizer: 糧食研究会報告会
• [Presentation] Deciphering the mystery of sleep: toward the molecular substrate for sleepiness (2021)
  • Author(s): 柳沢正史
  • Organizer: Grand Rounds to the Massachusetts General Hospital/ Harvard Medical School
  • Int'l Joint Research / Invited
• [Presentation] 睡眠覚醒の謎に挑む～「眠気」の正体を求めて～ (2021)
  • Author(s): 柳沢正史
  • Organizer: CVMW2021合同シンポジウム,第25回日本心血管内分泌代謝学会学術総会 他
  • Int'l Joint Research / Invited