2018 Fiscal Year Final Research Report
Investigation of plasma pathogenic factor in Idiopathic nephrotic syndrome using humanized-mouse and flow cytometry analysis of plasma blood cells
| Project/Area Number |
17H06658
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| Research Category |
Grant-in-Aid for Research Activity Start-up
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| Allocation Type | Single-year Grants |
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| Research Field |
Pediatrics
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| Research Institution | Tokai University (2018)
Tokyo Medical and Dental University (2017) |
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Principal Investigator |
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| Project Period (FY) |
2017-08-25 – 2019-03-31
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| Keywords | Nephrotic syndrome / Podocyte / フローサイトメトリー / ポドサイト / 液性因子 |
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| Outline of Final Research Achievements |
In this present study, to elucidate both a therapeutic target and a permeability factor of the idiopathic nephrotic syndrome in children, we analyzed comprehensive distributions of peripheral blood cells in patient with that disease. It is necessary to analyze a more large samples to get a meaningful result. We confirmed the alteration of cyteskeletal proteins induced by cytotoxic agent using immortalized podocyte cell line and mouse kidney organoid derived from nephron progenitor cells. This study was thought to become a step of elucidation of pathogenic permeability factor of the idiopathic nephrotic syndrome in children.
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| Free Research Field |
Pediatric Nephrology
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| Academic Significance and Societal Importance of the Research Achievements |
特発性ネフローゼ症候群は原因が未解明な疾患である。ステロイド治療で生じる易感染症、低身長など特徴的副作用を回避するため、病因を解明し新たな治療戦略を確立することが、医学的に重要である。本研究では、特発性ネフローゼ症候群患者の末梢血における血球分画をフローサイトメトリー解析により患者特有の血球分布の傾向を見出した。また機能解析に有用な培養糸球体上皮細胞や腎臓オルガノイドを用いた実験系を確立した。今後患者検体の蓄積と機能解析を進めることで、当初の目的をさらに明らかにすることが期待される。
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