Study of FGF2 Responsive Genes in human Dental Pulp Cells Facilitating Recovery in a Spinal Cord Injury Model

2018 Fiscal Year Final Research Report

• Project/Area Number: 17H06731
• Research Category: Grant-in-Aid for Research Activity Start-up
• Allocation Type: Single-year Grants
• Research Field: Dental engineering/Regenerative dentistry
• Research Institution: Gifu University
• Principal Investigator: Ken Sugiyama 岐阜大学, 医学部附属病院, 助教 (30802673)
• Project Period (FY): 2017-08-25 – 2019-03-31
• Keywords: ヒト歯髄細胞 / FGF2 / 脊髄損傷 / GABRB1

Outline of Final Research Achievements

The central nervous system in adult mammals does not heal spontaneously after spinal cord injury (SCI). We recently reported that FGF2-pretreated human dental pulp cells (hDPCs) can improve recovery in a rat model of SCI. This study aimed to investigate mechanisms underlying the curative effect of SCI enhanced via FGF2 pretreatment; we selected three hDPC lines upon screening for the presence of mesenchymal stem cell markers and of their functionality in a rat model of SCI. We identified FGF2-responsive genes via gene expression analyses in these lines. FGF2 treatment upregulated GABRB1, MMP1, and DRD2, which suggested to contribute to SCI or central the nervous system. In an expanded screening of additional lines, GABRB1 displayed rather unique and interesting behavior; two lines with the lowest sensitivity of GABRB1 to FGF2 treatment displayed an extremely minor effect in the SCI model.

Free Research Field

歯学