Control of protein function by self-assembled artificial organelle

2018 Fiscal Year Final Research Report

• Project/Area Number: 17H06759
• Research Category: Grant-in-Aid for Research Activity Start-up
• Allocation Type: Single-year Grants
• Research Field: Bio-related chemistry
• Research Institution: Nagoya Institute of Technology
• Principal Investigator: Yoshii Tatsuyuki 名古屋工業大学, 工学(系)研究科(研究院), 助教 (30778048)
• Project Period (FY): 2017-08-25 – 2019-03-31
• Keywords: 細胞 / オルガネラ / 自己組織化

Outline of Final Research Achievements

We have developed protein-based synthetic organelles in living mammalian cells. Chemogenetic protein-releasing/trapping system into the synthetic organelle was developed. Protein activity was inactivated by trapping into the synthetic organelles due to the isolation from the substrates. Releasing of the proteins from the synthetic organelles resulted in the activation of the downstream signaling.

Free Research Field

ケミカルバイオロジー

Academic Significance and Societal Importance of the Research Achievements

本研究により、さまざまな細胞内のタンパク質の機能を自由に制御することが可能になった。本技術は、基礎的な細胞生物学における研究ツールだけではなく、幹細胞の分化制御や細胞治療などへの応用が期待される。