2019 Fiscal Year Annual Research Report

共生性渦鞭毛藻類　Ａｍｐｈｉｄｉｎｉｕｍｓｐ．　のゲノムおよび毒素生合成

Research Project

Project/Area Number	17J00597
Research Institution	Okinawa Institute of Science and Technology Graduate University
Principal Investigator	BEEDESSEE Girish 沖縄科学技術大学院大学, 科学技術研究科, 特別研究員(DC1)
Project Period (FY)	2017-04-26 – 2020-03-31
Keywords	dinoflagellate / isoseq / seondary metabolism
Outline of Annual Research Achievements	We completed the genome of Amphidinium gibbosum and using an integrated omics approach, we surveyed the mechanisms of secondary metabolite biosynthesis. We combined genomics, transcriptomics and microRNAomics using sequencing data from Illumina and Pacbio.We identified key enzymes needed to make secondary metabolites.We also found that under nutrient conditions ( nitrate), secondary metabolites biosynthesis is downregulated because of the probably role of a miRNA.Such information is helpful in helping understand harmful algal bloom formation.We also identified several molecular events such as alternative splicing in secondary metabolite genes, that affect their expression. Taken together, we propose 2 strategies for the synthesis of secondary metabolites in dinoflagellates that include iterative use of enzymes.
Research Progress Status	令和元年度が最終年度であるため、記入しない。
Strategy for Future Research Activity	令和元年度が最終年度であるため、記入しない。