2017 Fiscal Year Research-status Report
Establishment of DNA adductomics to study human cells
| Project/Area Number |
17K00555
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| Research Institution | Yokohama City University |
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Principal Investigator |
Robert Kanaly 横浜市立大学, 生命ナノシステム科学研究科(八景キャンパス), 教授 (00448656)
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| Project Period (FY) |
2017-04-01 – 2020-03-31
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| Keywords | DNA adductomics |
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| Outline of Annual Research Achievements |
Designed a cell culturing system for human cell lines, purchased equipment, and built a system that allows for aseptic handling and storage of cells, cell culturing, and exposure of cells to hazardous chemicals. Developed methods for the extraction of DNA from human cells, digestion of DNA and preparation for LC/ESI-MS/MS analyses. Methods for LC/ESI-MS/MS analyses were developed. DNA adductomics analyses of Hep G2 cells were conducted after exposure to the dietary-related, monoaromatic chemical, safrole. Comparative analyses through the construction of DNA adductome maps revealed safrole-derived DNA adducts and preliminary identification of such adducts was conducted by LC/ESI-MS/MS product ion scan fragmentation analyses. Results of the first years' work were presented at two national meetings.
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| Current Status of Research Progress |
Current Status of Research Progress
2: Research has progressed on the whole more than it was originally planned.
Reason
Cell culturing, handling, and exposure system was built and methods for DNA extraction, digestion and LC/ESI-MS/MS analyses were established.
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| Strategy for Future Research Activity |
Continue DNA adductome analyses using model toxicants such as 2,6-dimethylaniline as described in grant proposal. Continue DNA adduct identification analyses. Explore the possibility of applying our methods to conduct "RNA adductomics" using our human cell culturing and exposure system.
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| Causes of Carryover |
Materials for continuation of experimentation and analyses. Publication fees. Budget for travel to national and international conferences.
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Research Products
(3 results)
