2018 Fiscal Year Research-status Report
Establishment of DNA adductomics to study human cells
| Project/Area Number |
17K00555
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| Research Institution | Yokohama City University |
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Principal Investigator |
Robert Kanaly 横浜市立大学, 生命ナノシステム科学研究科(八景キャンパス), 教授 (00448656)
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| Project Period (FY) |
2017-04-01 – 2020-03-31
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| Keywords | DNA adductomics |
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| Outline of Annual Research Achievements |
DNA adductome analyses of Hep G2 cells exposed to the monoaromatic chemicals, safrole and monoaromatic amines were conducted including DNA adductome mapping and LC/ESI-MS/MS CID analyses of DNA adduct candidates. Research results related to safrole exposure were finalized and submitted for peer review. Methods were established (1) for Hep G2 cell viability assays, (2) for in vitro monoaromatic chemical reaction assays with S9, extraction, and LC/ESI-MS/MS analyses, (3) for adduct synthesis of a monoaromatic amine DNA adduct, and (4) for cultivation of a human bladder cell line. Methods for "RNA adductomics" were developed and some experiments were completed.
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| Current Status of Research Progress |
Current Status of Research Progress
2: Research has progressed on the whole more than it was originally planned.
Reason
Finalized and submitted results for peer review of some of the work conducted from 2017 to 2018.
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| Strategy for Future Research Activity |
(1) Conduct DNA adductome analyses using bladder cell line, (2) continue syntheses of DNA adduct standards and apply to DNA adductomics, (3) develop high resolution mass spectrometry HRMS approaches to apply to adductomics, (4) continue development of "RNA adductomics".
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| Causes of Carryover |
Materials for continuation of experiments, possible publication fees, travel fees for conference presentations.
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