2019 Fiscal Year Annual Research Report
Establishment of DNA adductomics to study human cells
| Project/Area Number |
17K00555
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| Research Institution | Yokohama City University |
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Principal Investigator |
Robert Kanaly 横浜市立大学, 理学部, 教授 (00448656)
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| Project Period (FY) |
2017-04-01 – 2020-03-31
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| Keywords | Adductomics |
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| Outline of Annual Research Achievements |
Nucleic acid adductomics analyses utilizing human cell lines were continued for DNA adductomics and DNA/RNA adductomics. High resolution-(HR-)MS methodologies were successfully applied in conjunction with traditional adductomics approaches. Exposure assays that utilized the model 2'-deoxynucleoside, 2'-deoxyguanosine were conducted using 1,2-naphthoquinone and arylhydroxylamines and analyzed by LC/ESI-MS/MS, LC/ESI-HR/MS and NMR. Syntheses, purification and confirmation of arylhydroxylamine adducts were conducted.
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Research Products
(11 results)
