2017 Fiscal Year Research-status Report
Developing chemical methods for removing unwanted metals from the environment using plants
Project/Area Number |
17K00608
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Research Institution | Institute of Physical and Chemical Research |
Principal Investigator |
申 怜 国立研究開発法人理化学研究所, 環境資源科学研究センター, ユニットリーダー (80525577)
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Project Period (FY) |
2017-04-01 – 2020-03-31
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Keywords | Phytoremediation / heavy metals / methyl cysteinate |
Outline of Annual Research Achievements |
To understand the site of actions (outside of plant cells/surface of plants/inside of plant cells) for 15 already identified chemicals (C17H19F3N2O2, C21H27N5OS2, C19H21N7S3, C27H29N3O3, C24H24N4Br2, C13H15N5, C16H11ClO4, C11H15NO5S2, C4H9NO2S, C11H14N2O2S, C20H19BrN2O, C14H22N2O, C9H13BrN2O2, C9H11N3OS2 and C11H11N3OS2), the transfer experiments were performed. In these experiments Arabidopsis seeds were grown in the absence or presence of each chemical, seedlings were transferred onto fresh media in the absence or presence of cesium and analyzed. From these experiments, several chemicals (C4H9NO2S, C11H15NO5S2, C16H11ClO4, C13H15N5, C11H14N2O2S, C20H19BrN2O, C14H22N2O, C9H13BrN2O2, C9H11N3OS2 and C11H11N3OS2) were confirmed they function inside of plant cells. The chemicals function in inside of plants cells or surface of plants were tested whether they have effects on the plant phenotype (alter the sensitivities to heavy metals by the chemical) and whether they also helps to enhance the accumulation of various metals (lead, cadmium, aluminum, potassium and cesium) in plants. Among the tested chemicals, C4H9NO2S helped plants to accumulate more cesium and lead but not cadmium in plants without changing of phenotype in heavy metals available condition. Also, the physic-based theoretical modeling (quantum mechanical calculations) suggested this chemical could bind on cesium as well as lead. Also the transfer experiments with various heavy metals suggested that C4H9NO2S functions inside of plant cells for helping plants to accumulate more lead in plants.
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Current Status of Research Progress |
Current Status of Research Progress
2: Research has progressed on the whole more than it was originally planned.
Reason
As we planned, 15 chemicals (C17H19F3N2O2, C21H27N5OS2, C19H21N7S3, C27H29N3O3, C24H24N4Br2, C13H15N5, C16H11ClO4, C11H15NO5S2, C4H9NO2S, C11H14N2O2S, C20H19BrN2O, C14H22N2O, C9H13BrN2O2, C9H11N3OS2 and C11H11N3OS2) were tested whether they function inside plant cells or outside plant cells. Also, selected chemicals (focusing on chemicals function in the inside of plant cells such as C16H11ClO4 and C4H9NO2S) were accordingly tested their abilities of lead, cadmium and aluminum accumulation. The research progress in FY2017 was well followed as the research plans that we described in the grant proposal.
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Strategy for Future Research Activity |
1)Analyzation of the candidate chemicals for metal accumulation effect (FY2018) The selected candidate chemicals (from FY2017 outcome) which have the effect on altering metal accumulation in plants will be tested other heavy metals accumulation abilities. The derivatives of candidate chemicals will be tested whether they also alter the metal accumulation abilities in plants. 2) Identification of selected chemicals binding proteins (FY2018) In order to isolate candidate chemical binding proteins, chemical binding assay will be performed to isolate proteins which bind with the selected chemicals. The method to immobilize a chemical onto the surface of sepharose beads will be incubated with plant extract and chemical binding proteins will be purified. The identification of resultant proteins will be determined using in-gel peptide sequencing method. 3)Analyzation of relationships between target proteins and chemicals and functional studies in planta (FY2019) Once the chemical binding candidate proteins are revealed, whether the interactions between the chemicals and target proteins are affected by metals, their binding assays in various metals will be performed. If their interactions are regulated by metals, the gene expression analysis will be performed. The next plans will include the functional analysis of selected target protein. The knockout/overexpression plants of promising target proteins will be created and their metal accumulation abilities will be tested compared to wild type plants upon chemical treatments.
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Research Products
(1 results)