2019 Fiscal Year Annual Research Report
Developing chemical methods for removing unwanted metals from the environment using plants
Project/Area Number |
17K00608
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Research Institution | Institute of Physical and Chemical Research |
Principal Investigator |
申 怜 国立研究開発法人理化学研究所, 環境資源科学研究センター, ユニットリーダー (80525577)
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Project Period (FY) |
2017-04-01 – 2020-03-31
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Keywords | phytoremediation / lead / cesium / cadmium / selenium / glucosidase / chemical screening |
Outline of Annual Research Achievements |
1) Analyzation of relationships between target proteins and chemicals for metal accumulation We successfully identified beta-glucosidase as a C17H19F3N2O2 binding protein and a multi-domain-including protein as a C4H9NO2S binding protein. In the knockout plants of a beta-glucosidase showed the no further metal accumulation even though C17H19F3N2O2 applications. This finding suggested that a beta-glucosidase is critical for the function of C17H19F3N2O2 in plants and is the real target protein of C17H19F3N2O2. However, the mutation of a multi-domain-including protein that is a target of C4H9NO2S showed only minor effect in plants. This may due to the large family members of multi-domain-including protein in plants. In addition, the expression of beta-glucosidase was increased by multiple metal treatments including cesium, selenium and cadmium. This finding gave a hint the interaction between a beta-glucosidase and C17H19F3N2O2 was transcriptionally regulated.
2) Functional analysis of each target proteins in planta The overexpression plants of a beta-glucosidase showed more cesium and cadmium accumulation when C17H19F3N2O2 was applied. However, there was no significant difference of metal accumulation without C17H19F3N2O2 applications. However, the overexpression of a multi-domain-including protein didn't give any further metal accumulation with or without C4H9NO2S application in plants. These findings suggested the new direction to improve the efficiency of phytoremediation.
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Research Products
(3 results)