2019 Fiscal Year Final Research Report
Study on production and functional analysis of alginate deoxysugars as new rare sugars
Project/Area Number |
17K00852
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Research Category |
Grant-in-Aid for Scientific Research (C)
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Allocation Type | Multi-year Fund |
Section | 一般 |
Research Field |
Eating habits
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Research Institution | Mie University |
Principal Investigator |
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Project Period (FY) |
2017-04-01 – 2020-03-31
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Keywords | アルギン酸デオキシ糖 / DEH / KDG / DEHレダクターゼ / Formosa haliotis / Caenorhabditis elegans |
Outline of Final Research Achievements |
4-Deoxy-L-erythro-5-hexoseulose uronic acid (DEH) and 2-keto-3-deoxy-D-gluconic acid (KDG) are rare sugars produced from alginate by the action of lyase and reductase. In this study, we obtained sequence information of a novel DEH reductase gene from genomic DNA of alginate-utilizing bacteria, and developed a new method for producing KDG from DEH using the protein expression system in Escherichia coli. In addition, it was clarified that DEH has a physiological action of anti-aging effect, enhances stress tolerance and suppresses the progression of neurodegenerative diseases (polyglutamine diseases).
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Free Research Field |
食品化学
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Academic Significance and Societal Importance of the Research Achievements |
DEHとKDGは,食用多糖類のアルギン酸から得られる希少糖である.DEHに加えKDGについても,1ステップの工程で純度約95%以上の産物を生産出来る方法を世界に先駆けて開発した.DEHは,生理機能としてアンチエイジング活性やストレス耐性の上昇効果,神経変異性疾患の進行を抑制する効果を持つことを明らかにした.したがって,DEHとKDG,この二つのアルギン酸デオキシ糖は,薬理効果を持つ新しい機能性単糖としての利用が期待される.
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