Differentiation Induction of Human Mesenchymal Stem Cells by Graphene Attached Hard/Soft Hybrid Structure Substrates

2019 Fiscal Year Final Research Report

• Project/Area Number: 17K05009
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Nanomaterials engineering
• Research Institution: Nippon Institute of Technology
• Principal Investigator: Ban Masahito 日本工業大学, 基幹工学部, 教授 (70424059)
• Co-Investigator(Kenkyū-buntansha): 沖川 侑揮 国立研究開発法人産業技術総合研究所, 材料・化学領域, 研究員 (50635315) ; 石原 正統 国立研究開発法人産業技術総合研究所, 材料・化学領域, 主任研究員 (70356450)
• Project Period (FY): 2017-04-01 – 2020-03-31
• Keywords: グラフェン / 幹細胞 / 増殖 / 分化

Outline of Final Research Achievements

Hard/soft hybrid structure substrates attached graphene with discriminating properties, extremely thin sheet-like structure, high elastic modulus and electric conductivity, were fabricated, and using these as the scaffolds culture tests of human mesenchymal stem cells were conducted. The substrates were obtained by fractionating the attached graphene into fine domains by means of applying a tensile strain. It was found that the graphene had a profound effect on promotion of cell proliferation at various conditions, substrates with different modulus, and basic medium and neuronal differentiation induction medium. Graphene did not indicate a significant effect on induction of differentiation into neuronal cells.

Free Research Field

材料工学

Academic Significance and Societal Importance of the Research Achievements

細胞足場として使用する基質の表面にグラフェンを付着させることで、ヒト間葉系幹細胞を大幅に増殖させることできることが明らかになった。この効果は、基質が軟質なPDMSだけでなくガラス基板においても、また、基本培地のほか分化誘導培地を使用した場合においても認められた。このことは、グラフェンが、ヒト間葉系幹細胞細胞の高効率な増殖用足場として使用できる可能性を示しており、培養時間の短縮を図るための有力なツールになることが期待できる。