Influence of plasma irradiation on biochemical reactions

2019 Fiscal Year Final Research Report

• Project/Area Number: 17K05095
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Plasma electronics
• Research Institution: Toyohashi University of Technology
• Principal Investigator: Kurita Hirofumi 豊橋技術科学大学, 工学(系)研究科(研究院), 助教 (70512177)
• Project Period (FY): 2017-04-01 – 2020-03-31
• Keywords: プラズマ応用 / プラズマ医療 / DNA損傷 / 生体分子損傷

Outline of Final Research Achievements

Influence of plasma irradiation on biochemical reactions were investigated. First of all, model enzymes were dissolved in an aqueous solution and then irradiated to an atmospheric pressure plasma jet. Plasma irradiation resulted in decrease in enzymatic activity, and reactive species were produced in the aqueous solution as predominant factors. The inactivation process was difference from that of heat denaturation. In addition, the results suggest that plasma irradiation of cultured human lung cancer cell lines may be involved in the induction of apoptosis. In addition, DNA damage in the A549 human lung cancer cell line treated with cold plasma irradiation was investigated. 8-oxoguanine (8-oxoG) formation as well as DNA strand breaks, which have been thoroughly investigated, were induced by plasma irradiation. In addition, up-regulation of 8-oxoG repair enzyme was observed after plasma irradiation.

Free Research Field

プラズマ応用

Academic Significance and Societal Importance of the Research Achievements

大気圧プラズマが生化学反応に及ぼす影響を調べ、プラズマ照射により損傷を受けた生体分子が修復される生化学反応が細胞内で起こっていることが明らかになった。このときの細胞生存率はプラズマ未照射と比較してほとんど低下しておらず、一見プラズマ照射の影響がないような照射であっても細胞内の生体分子は損傷を受けており、細胞自身がそれを修復していることが示唆された。