Fluorescence Detection of Saccharide Based on a Complexation between Aggregate Emission Dye and Lectin

2019 Fiscal Year Final Research Report

• Project/Area Number: 17K05849
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Functional solid state chemistry
• Research Institution: Kurume National College of Technology
• Principal Investigator: Ishi-i Tsutomu 久留米工業高等専門学校, 生物応用化学科, 教授 (60346856)
• Project Period (FY): 2017-04-01 – 2020-03-31
• Keywords: 発光 / 会合 / ドナー・アクセプター / 糖質 / レクチン / 生体検出

Outline of Final Research Achievements

In this study, we report fluorescence detection of saccharide based on a complexation between aggregate emission dye and lectin. The dye bearing a saccharide moiety, which is fluorescent in the aggregate state, indicates a fluorescence quenching in the monomer state after the complexation with lectin. In contrast, an emission recovery could be achieved by treatment with target saccharide, as a result of the dissociation of the complex between dye and lectin, and the subsequent aggregate formation of dye molecules. The present exchange between aggregate emission and monomer quenching can be applied to the fluorescence detection of target saccharide.

Free Research Field

有機機能物性化学　有機構造化学　有機光化学

Academic Significance and Societal Importance of the Research Achievements

本研究では、レクチンを用いる糖質蛍光検出に、会合発光というアイデアを付与することで、単純で合理的な戦略を提案している。単純な色素基盤とレクチンとの複合体を用いて、蛍光 OFF-ON 変化で糖質を検出する基礎的な研究内容であるが、レクチン－糖質の組合せに留まらず、抗原―抗体等の様々な生体アフィニティー系での蛍光検出の可能性を秘めた研究でもあり、基礎から応用までの一貫した研究の基盤を確立できる。